LSH3_ARATH
ID LSH3_ARATH Reviewed; 219 AA.
AC O82268;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Protein LIGHT-DEPENDENT SHORT HYPOCOTYLS 3;
DE AltName: Full=Protein ORGAN BOUNDARY 1;
GN Name=LSH3; Synonyms=OBO1; OrderedLocusNames=At2g31160; ORFNames=T16B12.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=14871309; DOI=10.1111/j.1365-313x.2003.01993.x;
RA Zhao L., Nakazawa M., Takase T., Manabe K., Kobayashi M., Seki M.,
RA Shinozaki K., Matsui M.;
RT "Overexpression of LSH1, a member of an uncharacterised gene family, causes
RT enhanced light regulation of seedling development.";
RL Plant J. 37:694-706(2004).
RN [5]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=21435050; DOI=10.1111/j.1365-313x.2011.04571.x;
RA Takeda S., Hanano K., Kariya A., Shimizu S., Zhao L., Matsui M., Tasaka M.,
RA Aida M.;
RT "CUP-SHAPED COTYLEDON1 transcription factor activates the expression of
RT LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary
RT cells.";
RL Plant J. 66:1066-1077(2011).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, AND GENE FAMILY.
RX PubMed=21245300; DOI=10.1073/pnas.1018542108;
RA Cho E., Zambryski P.C.;
RT "Organ boundary1 defines a gene expressed at the junction between the shoot
RT apical meristem and lateral organs.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:2154-2159(2011).
RN [7]
RP DNA-BINDING, AND GENE FAMILY.
RX PubMed=23146749; DOI=10.1186/1745-6150-7-39;
RA Iyer L.M., Aravind L.;
RT "ALOG domains: provenance of plant homeotic and developmental regulators
RT from the DNA-binding domain of a novel class of DIRS1-type retroposons.";
RL Biol. Direct 7:39-39(2012).
CC -!- FUNCTION: Probable transcription regulator that acts as a developmental
CC regulator by promoting cell growth in response to light (By
CC similarity). Required for the maintenance of the shoot apical meristem
CC and for the formation of lateral organs. Promotes petal formation and
CC growth, but may suppress organ differentiation in the boundary region.
CC {ECO:0000250, ECO:0000269|PubMed:21245300}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21245300,
CC ECO:0000269|PubMed:21435050}.
CC -!- TISSUE SPECIFICITY: Expressed around apical meristems, at the
CC boundaries of vegetative and reproductive organs. Induced by
CC NAC054/CUC1 and NAC098/CUC2 in shoot organ boundary cells.
CC {ECO:0000269|PubMed:21245300, ECO:0000269|PubMed:21435050}.
CC -!- DEVELOPMENTAL STAGE: Accumulates at the boundaries between the apical
CC meristems and lateral organs in embryos, seedlings, and mature plants,
CC and at the root apical meristem and in distinct cell files surrounding
CC this area. First observed in heart-stage embryos, in the cotyledon
CC boundary region of the shoot apex, and in the quiescent center and
CC columella initial cells at the root tip. In bending-cotyledon stage
CC embryos, weakly expressed in the boundary cells between the shoot
CC apical meristem (SAM) and the cotyledons. In seedlings, accumulates in
CC cells between the SAM and the cotyledons. During the reproductive
CC phase, restricted to the basal regions of the pedicels and floral
CC organs. In the inflorescence shoot apex, expressed in the early flower
CC primordia, but not in the SAM. Later present in the cryptic bract
CC region, but not in the floral meristem. {ECO:0000269|PubMed:21245300,
CC ECO:0000269|PubMed:21435050}.
CC -!- DISRUPTION PHENOTYPE: No phenotype, probably due to redundant function
CC of paralogs. {ECO:0000269|PubMed:21245300}.
CC -!- SIMILARITY: Belongs to the plant homeotic and developmental regulators
CC ALOG protein family. {ECO:0000305}.
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DR EMBL; AC005311; AAC63835.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08501.1; -; Genomic_DNA.
DR EMBL; AY056383; AAL08239.1; -; mRNA.
DR EMBL; AY081705; AAL87358.1; -; mRNA.
DR PIR; C84717; C84717.
DR RefSeq; NP_565716.1; NM_128672.2.
DR AlphaFoldDB; O82268; -.
DR BioGRID; 3020; 2.
DR IntAct; O82268; 1.
DR STRING; 3702.AT2G31160.1; -.
DR PaxDb; O82268; -.
DR PRIDE; O82268; -.
DR ProteomicsDB; 238594; -.
DR EnsemblPlants; AT2G31160.1; AT2G31160.1; AT2G31160.
DR GeneID; 817672; -.
DR Gramene; AT2G31160.1; AT2G31160.1; AT2G31160.
DR KEGG; ath:AT2G31160; -.
DR Araport; AT2G31160; -.
DR TAIR; locus:2055897; AT2G31160.
DR eggNOG; ENOG502QT0B; Eukaryota.
DR HOGENOM; CLU_071168_1_0_1; -.
DR InParanoid; O82268; -.
DR OMA; AYEENGW; -.
DR OrthoDB; 1349135at2759; -.
DR PhylomeDB; O82268; -.
DR PRO; PR:O82268; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82268; baseline and differential.
DR Genevisible; O82268; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0010492; P:maintenance of shoot apical meristem identity; IMP:UniProtKB.
DR GO; GO:0009299; P:mRNA transcription; ISS:UniProtKB.
DR GO; GO:0010199; P:organ boundary specification between lateral organs and the meristem; IMP:UniProtKB.
DR GO; GO:0048441; P:petal development; IMP:UniProtKB.
DR GO; GO:0090698; P:post-embryonic plant morphogenesis; IMP:UniProtKB.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR GO; GO:0048834; P:specification of petal number; IMP:UniProtKB.
DR InterPro; IPR040222; ALOG.
DR InterPro; IPR006936; ALOG_dom.
DR PANTHER; PTHR31165; PTHR31165; 1.
DR Pfam; PF04852; DUF640; 1.
DR PROSITE; PS51697; ALOG; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..219
FT /note="Protein LIGHT-DEPENDENT SHORT HYPOCOTYLS 3"
FT /id="PRO_0000425290"
FT DOMAIN 54..181
FT /note="ALOG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01033"
FT REGION 31..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 172..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 179..183
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 31..54
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 219 AA; 24123 MW; 57C7DFE1537FCA5D CRC64;
MDMIPQLMEG SSAYGGVTNL NIISNNSSSV TGATGGEATQ PLSSSSSPSA NSSRYENQKR
RDWNTFGQYL RNHRPPLSLS RCSGAHVLEF LRYLDQFGKT KVHTNICHFY GHPNPPAPCP
CPLRQAWGSL DALIGRLRAA FEENGGKPET NPFGARAVRL YLREVRDMQS KARGVSYEKK
KRKRPLPSSS TSSSSAVASH QQFQMLPGTS STTQLKFEK
