LSH4_ARATH
ID LSH4_ARATH Reviewed; 195 AA.
AC Q9LW68; Q6NNL1;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Protein LIGHT-DEPENDENT SHORT HYPOCOTYLS 4;
DE AltName: Full=Protein ORGAN BOUNDARY 4;
GN Name=LSH4; Synonyms=OBO4; OrderedLocusNames=At3g23290; ORFNames=MLM24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-96.
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=14871309; DOI=10.1111/j.1365-313x.2003.01993.x;
RA Zhao L., Nakazawa M., Takase T., Manabe K., Kobayashi M., Seki M.,
RA Shinozaki K., Matsui M.;
RT "Overexpression of LSH1, a member of an uncharacterised gene family, causes
RT enhanced light regulation of seedling development.";
RL Plant J. 37:694-706(2004).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=21435050; DOI=10.1111/j.1365-313x.2011.04571.x;
RA Takeda S., Hanano K., Kariya A., Shimizu S., Zhao L., Matsui M., Tasaka M.,
RA Aida M.;
RT "CUP-SHAPED COTYLEDON1 transcription factor activates the expression of
RT LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary
RT cells.";
RL Plant J. 66:1066-1077(2011).
RN [7]
RP GENE FAMILY.
RX PubMed=21245300; DOI=10.1073/pnas.1018542108;
RA Cho E., Zambryski P.C.;
RT "Organ boundary1 defines a gene expressed at the junction between the shoot
RT apical meristem and lateral organs.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:2154-2159(2011).
RN [8]
RP DNA-BINDING, AND GENE FAMILY.
RX PubMed=23146749; DOI=10.1186/1745-6150-7-39;
RA Iyer L.M., Aravind L.;
RT "ALOG domains: provenance of plant homeotic and developmental regulators
RT from the DNA-binding domain of a novel class of DIRS1-type retroposons.";
RL Biol. Direct 7:39-39(2012).
CC -!- FUNCTION: Probable transcription regulator that acts as a developmental
CC regulator by promoting cell growth in response to light (By
CC similarity). May suppress organ differentiation in the boundary region.
CC {ECO:0000250, ECO:0000269|PubMed:21435050}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21435050}.
CC -!- TISSUE SPECIFICITY: Induced by NAC054/CUC1 and NAC098/CUC2 in shoot
CC organ boundary cells. {ECO:0000269|PubMed:21435050}.
CC -!- DEVELOPMENTAL STAGE: Accumulates at the boundaries between the apical
CC meristems and lateral organs in embryos, seedlings, and mature plants,
CC and at the root apical meristem and in distinct cell files surrounding
CC this area. First observed in the shoot apex of early-heart embryos.
CC Within the apex of the late-heart stage embryo, the signal is detected
CC in the peripheral region but not in the central region. At the bent-
CC cotyledon stage, accumulates in boundary cells located between the
CC shoot apical meristem (SAM) and the cotyledon primordia. In seedlings,
CC weakly expressed in the basal cells of the leaf primordia. During the
CC reproductive phase, present in boundary cells between the SAM and the
CC flower primordia, and in boundary cells between the floral meristem and
CC sepal primordia. In developing flowers, confined to regions that
CC surround the floral organs at the base. {ECO:0000269|PubMed:21435050}.
CC -!- SIMILARITY: Belongs to the plant homeotic and developmental regulators
CC ALOG protein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR24161.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB015474; BAB02272.1; -; Genomic_DNA.
DR EMBL; AP000733; BAB02272.1; JOINED; Genomic_DNA.
DR EMBL; CP002686; AEE76748.1; -; Genomic_DNA.
DR EMBL; BT010794; AAR24161.1; ALT_INIT; mRNA.
DR EMBL; BT011275; AAR92311.1; -; mRNA.
DR RefSeq; NP_001078202.2; NM_001084733.2.
DR AlphaFoldDB; Q9LW68; -.
DR BioGRID; 7240; 3.
DR IntAct; Q9LW68; 3.
DR STRING; 3702.AT3G23290.2; -.
DR PaxDb; Q9LW68; -.
DR PRIDE; Q9LW68; -.
DR ProteomicsDB; 238531; -.
DR EnsemblPlants; AT3G23290.2; AT3G23290.2; AT3G23290.
DR GeneID; 821908; -.
DR Gramene; AT3G23290.2; AT3G23290.2; AT3G23290.
DR KEGG; ath:AT3G23290; -.
DR Araport; AT3G23290; -.
DR TAIR; locus:2082170; AT3G23290.
DR eggNOG; ENOG502QT0B; Eukaryota.
DR HOGENOM; CLU_071168_1_0_1; -.
DR InParanoid; Q9LW68; -.
DR OMA; MELSPPN; -.
DR OrthoDB; 1337441at2759; -.
DR PhylomeDB; Q9LW68; -.
DR PRO; PR:Q9LW68; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LW68; baseline and differential.
DR Genevisible; Q9LW68; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009299; P:mRNA transcription; ISS:UniProtKB.
DR GO; GO:0090698; P:post-embryonic plant morphogenesis; IMP:UniProtKB.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR InterPro; IPR040222; ALOG.
DR InterPro; IPR006936; ALOG_dom.
DR PANTHER; PTHR31165; PTHR31165; 1.
DR Pfam; PF04852; DUF640; 1.
DR PROSITE; PS51697; ALOG; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..195
FT /note="Protein LIGHT-DEPENDENT SHORT HYPOCOTYLS 4"
FT /id="PRO_0000425291"
FT DOMAIN 48..175
FT /note="ALOG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01033"
FT REGION 28..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 162..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 173..177
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 28..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 162..179
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..195
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 195 AA; 21434 MW; A6BA85958BEFCE23 CRC64;
MDHIIGFMGT TNMSHNTNLM IAAAATTTTT SSSSSSSSGG SGTNQLSRYE NQKRRDWNTF
GQYLRNHRPP LSLSRCSGAH VLEFLRYLDQ FGKTKVHTHL CPFFGHPNPP APCACPLRQA
WGSLDALIGR LRAAFEENGG SPETNPFGAR AVRLYLREVR DSQAKARGIS YEKKKRKRPP
PPLPPAQPAI SSSPN
