LSHB_HORSE
ID LSHB_HORSE Reviewed; 169 AA.
AC P08751; P01234;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Lutropin/choriogonadotropin subunit beta;
DE AltName: Full=LSH-B/CG-B;
DE AltName: Full=Luteinizing hormone subunit beta;
DE AltName: Full=Lutropin/choriogonadotropin beta chain;
DE Flags: Precursor;
GN Name=LHB;
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1379674; DOI=10.1210/mend.6.6.1379674;
RA Sherman G.B., Wolfe M.W., Farmerie T.A., Clay C.M., Threadgill D.S.,
RA Sharp D.C., Nilson J.H.;
RT "A single gene encodes the beta-subunits of equine luteinizing hormone and
RT chorionic gonadotropin.";
RL Mol. Endocrinol. 6:951-959(1992).
RN [2]
RP PROTEIN SEQUENCE OF 21-169.
RX PubMed=3298239; DOI=10.1016/s0021-9258(18)47457-1;
RA Bousfield G.R., Liu W.-K., Sugino H., Ward D.N.;
RT "Structural studies on equine glycoprotein hormones. Amino acid sequence of
RT equine lutropin beta-subunit.";
RL J. Biol. Chem. 262:8610-8620(1987).
RN [3]
RP PROTEIN SEQUENCE OF 21-169.
RX PubMed=3298238; DOI=10.1016/s0021-9258(18)47456-x;
RA Sugino H., Bousfield G.R., Moore W.T. Jr., Ward D.N.;
RT "Structural studies on equine glycoprotein hormones. Amino acid sequence of
RT equine chorionic gonadotropin beta-subunit.";
RL J. Biol. Chem. 262:8603-8609(1987).
RN [4]
RP PARTIAL PROTEIN SEQUENCE.
RA Ward D.N., Moore W.T. Jr., Burleigh B.D.;
RT "Structural studies on equine chorionic gonadotropin.";
RL J. Protein Chem. 1:263-280(1982).
RN [5]
RP STRUCTURE OF CARBOHYDRATES.
RX PubMed=2331995; DOI=10.1111/j.1432-1033.1990.tb15474.x;
RA Damm J.B.L., Haard K., Kamerling J.P., van Dedem G.W.K.,
RA Vliegenthart J.F.G.;
RT "Structure determination of the major N- and O-linked carbohydrate chains
RT of the beta subunit from equine chorionic gonadotropin.";
RL Eur. J. Biochem. 189:175-183(1990).
RN [6]
RP GLYCOSYLATION AT SER-138; SER-143; THR-147; SER-148; THR-149; SER-150;
RP THR-151; THR-153; SER-157; SER-160; SER-161 AND SER-169.
RX PubMed=11133668; DOI=10.1095/biolreprod64.1.136;
RA Bousfield G.R., Butnev V.Y., Butnev V.Y.;
RT "Identification of twelve O-glycosylation sites in equine chorionic
RT gonadotropin beta and equine luteinizing hormone beta by solid-phase Edman
RT degradation.";
RL Biol. Reprod. 64:136-147(2001).
CC -!- FUNCTION: Promotes spermatogenesis and ovulation by stimulating the
CC testes and ovaries to synthesize steroids.
CC -!- SUBUNIT: Heterodimer of a common alpha chain and a unique beta chain
CC which confers biological specificity to thyrotropin, lutropin,
CC follitropin and gonadotropin.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- PTM: Microheterogeneity at Asn-33. O-glycosylation appears to be
CC responsible for the beta subunit contribution to the difference in LH-
CC receptor binding activity between LSH-B and CG-B.
CC {ECO:0000269|PubMed:11133668}.
CC -!- SIMILARITY: Belongs to the glycoprotein hormones subunit beta family.
CC {ECO:0000305}.
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DR EMBL; S41704; AAB22775.1; -; Genomic_DNA.
DR PIR; A41917; KTHOB.
DR RefSeq; NP_001184022.1; NM_001197093.1.
DR AlphaFoldDB; P08751; -.
DR SMR; P08751; -.
DR STRING; 9796.ENSECAP00000005559; -.
DR GlyConnect; 90; 5 N-Linked glycans, 9 O-Linked glycans.
DR iPTMnet; P08751; -.
DR PaxDb; P08751; -.
DR Ensembl; ENSECAT00000007582; ENSECAP00000005559; ENSECAG00000007548.
DR GeneID; 100054774; -.
DR KEGG; ecb:100054774; -.
DR CTD; 3972; -.
DR GeneTree; ENSGT00940000161285; -.
DR InParanoid; P08751; -.
DR OrthoDB; 1362225at2759; -.
DR Proteomes; UP000002281; Chromosome 10.
DR Bgee; ENSECAG00000007548; Expressed in trophoblast and 21 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00069; GHB_like; 1.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR006208; Glyco_hormone_CN.
DR InterPro; IPR001545; Gonadotropin_bsu.
DR InterPro; IPR018245; Gonadotropin_bsu_CS.
DR PANTHER; PTHR11515; PTHR11515; 1.
DR Pfam; PF00007; Cys_knot; 1.
DR SMART; SM00068; GHB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00261; GLYCO_HORMONE_BETA_1; 1.
DR PROSITE; PS00689; GLYCO_HORMONE_BETA_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000269|PubMed:3298238,
FT ECO:0000269|PubMed:3298239"
FT CHAIN 21..169
FT /note="Lutropin/choriogonadotropin subunit beta"
FT /id="PRO_0000011725"
FT REGION 131..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:3298238"
FT CARBOHYD 138
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 143
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 147
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 148
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 149
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 150
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 151
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 153
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 157
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 160
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 161
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT CARBOHYD 169
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:11133668"
FT DISULFID 29..77
FT /evidence="ECO:0000250"
FT DISULFID 43..92
FT /evidence="ECO:0000250"
FT DISULFID 46..130
FT /evidence="ECO:0000250"
FT DISULFID 54..108
FT /evidence="ECO:0000250"
FT DISULFID 58..110
FT /evidence="ECO:0000250"
FT DISULFID 113..120
FT /evidence="ECO:0000250"
SQ SEQUENCE 169 AA; 17865 MW; 1244ADBEB843EF1A CRC64;
METLQGLLLW MLLSVGGVWA SRGPLRPLCR PINATLAAEK EACPICITFT TSICAGYCPS
MVRVMPAALP AIPQPVCTYR ELRFASIRLP GCPPGVDPMV SFPVALSCHC GPCQIKTTDC
GVFRDQPLAC APQASSSSKD PPSQPLTSTS TPTPGASRRS SHPLPIKTS
