LSI2_ORYSJ
ID LSI2_ORYSJ Reviewed; 472 AA.
AC Q10SY9; Q8H7R0;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Silicon efflux transporter LSI2 {ECO:0000303|PubMed:17625566};
DE AltName: Full=Low silicon protein 2 {ECO:0000303|PubMed:17625566};
GN Name=LSI2 {ECO:0000303|PubMed:17625566};
GN Synonyms=SIET1 {ECO:0000303|PubMed:17625566};
GN OrderedLocusNames=Os03g0107300 {ECO:0000312|EMBL:BAF10605.1},
GN LOC_Os03g01700 {ECO:0000312|EMBL:ABF93544.1};
GN ORFNames=OJ1384D03.1 {ECO:0000312|EMBL:AAN62775.1},
GN OsJ_09099 {ECO:0000312|EMBL:EEE58171.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, AND MUTAGENESIS OF SER-115.
RC STRAIN=cv. Taichung 65;
RX PubMed=17625566; DOI=10.1038/nature05964;
RA Ma J.F., Yamaji N., Mitani N., Tamai K., Konishi S., Fujiwara T.,
RA Katsuhara M., Yano M.;
RT "An efflux transporter of silicon in rice.";
RL Nature 448:209-212(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Lemont;
RA Fang C.X., Wang Q.S., Yu Y., Li Q.M., Zhuang Y.E., Lin W.X.;
RT "Low silicon rice 2 (Lsi2) in UV-B resistant rice accession Lemont.";
RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=17905867; DOI=10.1104/pp.107.107599;
RA Ma J.F., Yamaji N., Tamai K., Mitani N.;
RT "Genotypic difference in silicon uptake and expression of silicon
RT transporter genes in rice.";
RL Plant Physiol. 145:919-924(2007).
RN [10]
RP FUNCTION.
RX PubMed=18626020; DOI=10.1073/pnas.0802361105;
RA Ma J.F., Yamaji N., Mitani N., Xu X.Y., Su Y.H., McGrath S.P., Zhao F.J.;
RT "Transporters of arsenite in rice and their role in arsenic accumulation in
RT rice grain.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:9931-9935(2008).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=26283388; DOI=10.1073/pnas.1508987112;
RA Yamaji N., Sakurai G., Mitani-Ueno N., Ma J.F.;
RT "Orchestration of three transporters and distinct vascular structures in
RT node for intervascular transfer of silicon in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:11401-11406(2015).
CC -!- FUNCTION: Silicon efflux transporter involved in silicon transport from
CC the root cells to the apoplast. Is coupled with the silicon influx
CC transporter NIP2-1/LSI1 in both exodermal and endodermal root cells for
CC an efficient silicon transport across the cells into the stele. Silicon
CC is beneficial to plant growth and helps plants to overcome abiotic and
CC biotic stresses by preventing lodging (falling over) and increasing
CC resistance to pests and diseases, as well as other stresses
CC (PubMed:17625566). In the nodes, involved with LSI3 and NIP2-2/LSI6 in
CC silicon intervascular transfer, which is required for the preferential
CC distribution of silicon, such as hyperaccumulation of silicon in the
CC husk (PubMed:26283388). Is coupled with NIP2-1/LSI1 transporter in
CC roots for efficient uptake of arsenite, which is further dispatched in
CC shoots and grains (PubMed:18626020). {ECO:0000269|PubMed:17625566,
CC ECO:0000269|PubMed:18626020, ECO:0000269|PubMed:26283388}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17625566};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Constitutively expressed in roots. Localizes on the
CC plasma membrane of the proximal side of both root exodermis and
CC endodermis, where casparian strips exist (at protein level).
CC {ECO:0000269|PubMed:17625566, ECO:0000269|PubMed:17905867}.
CC -!- INDUCTION: Regulated by silicon level; the expression being decreased
CC 4-fold by continuous silicon supply for 3 days.
CC {ECO:0000269|PubMed:17625566}.
CC -!- SIMILARITY: Belongs to the arsenite-antimonite (ArsB) efflux (TC
CC 2.A.45) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN62775.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB222273; BAF73750.1; -; mRNA.
DR EMBL; HM055577; ADH94038.1; -; mRNA.
DR EMBL; AC113930; AAN62775.1; ALT_INIT; Genomic_DNA.
DR EMBL; DP000009; ABF93544.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10605.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS81881.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE58171.1; -; Genomic_DNA.
DR EMBL; AK101092; BAG94909.1; -; mRNA.
DR RefSeq; XP_015632217.1; XM_015776731.1.
DR AlphaFoldDB; Q10SY9; -.
DR SMR; Q10SY9; -.
DR STRING; 4530.OS03T0107300-01; -.
DR TCDB; 2.A.45.2.4; the arsenite-antimonite (arsb) efflux family.
DR PaxDb; Q10SY9; -.
DR PRIDE; Q10SY9; -.
DR EnsemblPlants; Os03t0107300-01; Os03t0107300-01; Os03g0107300.
DR GeneID; 4331328; -.
DR Gramene; Os03t0107300-01; Os03t0107300-01; Os03g0107300.
DR KEGG; osa:4331328; -.
DR eggNOG; KOG2639; Eukaryota.
DR HOGENOM; CLU_011920_0_0_1; -.
DR InParanoid; Q10SY9; -.
DR OMA; IAMFIDV; -.
DR OrthoDB; 495573at2759; -.
DR BioCyc; MetaCyc:MON-21677; -.
DR PlantReactome; R-OSA-9618218; Arsenic uptake and detoxification.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR Pfam; PF03600; CitMHS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..472
FT /note="Silicon efflux transporter LSI2"
FT /id="PRO_0000440948"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 115
FT /note="S->N: In lsi2; impairs silicon uptake. Reduced
FT growth. Grain discoloration. Reduces grain yield 2.5-fold."
FT /evidence="ECO:0000269|PubMed:17625566"
SQ SEQUENCE 472 AA; 50359 MW; 071A52B843D766F9 CRC64;
MSELASAPKV ALGSIAFAVF WMMAVFPSVP FLPIGRTAGS LLSAVLMVIF HVISPDDAYA
SIDLPILGLL FATMVVGSYL RNAGMFKHLG RLLAWKSQGG RDLMCRVCVV TALASALFTN
DTCCVVLTEF VLELAAERNL PAKPFLLALA SSANIGSAAT PIGNPQNLVI AFNSKITFPK
FLMGILPAML VGMAVNMVML LCMYWRELGG GAELSVDGKQ MEAVEEGRSP ASAKSTPQLN
GNGNTMMSLE MSENITTKHP WFMQCTEARR KLFLKSFAYV VTVGMVVAYM VGLNMSWTAI
TTALALVVVD FRDAEPCLDT VSYSLLVFFS GMFITVSGFN KTGLPGAIWD FMAPYSKVNS
VGGISVLSVI ILLLSNLASN VPTVLLMGDE VAKAAALISP AAVTTSWLLL AWVSTVAGNL
SLLGSAANLI VCEQARRAPR NAYDLTFWQH IVFGVPSTLI VTAVGIPLIG KI
