LSI3_ORYSJ
ID LSI3_ORYSJ Reviewed; 485 AA.
AC Q9AV23; Q7XCH8;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Silicon efflux transporter LSI3 {ECO:0000303|PubMed:26283388};
DE AltName: Full=Low silicon protein 3 {ECO:0000303|PubMed:26283388};
GN Name=LSI3 {ECO:0000303|PubMed:26283388};
GN OrderedLocusNames=Os10g0547500 {ECO:0000312|EMBL:BAF27134.1},
GN LOC_Os10g39980 {ECO:0000312|EMBL:AAP54895.1};
GN ORFNames=OSJNBa0001O14.19 {ECO:0000312|EMBL:AAK20045.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=26283388; DOI=10.1073/pnas.1508987112;
RA Yamaji N., Sakurai G., Mitani-Ueno N., Ma J.F.;
RT "Orchestration of three transporters and distinct vascular structures in
RT node for intervascular transfer of silicon in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:11401-11406(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Silicon efflux transporter involved in silicon transport in
CC shoots. In the nodes, involved with LSI2 and NIP2-2/LSI6 in silicon
CC intervascular transfer, which is required for the preferential
CC distribution of silicon, such as hyperaccumulation of silicon in the
CC husk. Silicon is beneficial to plant growth and helps plants to
CC overcome abiotic and biotic stresses by preventing lodging (falling
CC over) and increasing resistance to pests and diseases, as well as other
CC stresses. {ECO:0000269|PubMed:26283388}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26283388};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the arsenite-antimonite (ArsB) efflux (TC
CC 2.A.45) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LC069370; BAS18932.1; -; mRNA.
DR EMBL; AC025783; AAK20045.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP54895.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF27134.1; -; Genomic_DNA.
DR EMBL; AP014966; BAT11916.1; -; Genomic_DNA.
DR EMBL; AK070040; BAG91738.1; -; mRNA.
DR RefSeq; XP_015614984.1; XM_015759498.1.
DR AlphaFoldDB; Q9AV23; -.
DR SMR; Q9AV23; -.
DR STRING; 4530.OS10T0547500-01; -.
DR PaxDb; Q9AV23; -.
DR PRIDE; Q9AV23; -.
DR EnsemblPlants; Os10t0547500-01; Os10t0547500-01; Os10g0547500.
DR GeneID; 4349293; -.
DR Gramene; Os10t0547500-01; Os10t0547500-01; Os10g0547500.
DR KEGG; osa:4349293; -.
DR eggNOG; KOG2639; Eukaryota.
DR HOGENOM; CLU_011920_0_0_1; -.
DR InParanoid; Q9AV23; -.
DR OMA; ISKGHAW; -.
DR OrthoDB; 495573at2759; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR Pfam; PF03600; CitMHS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..485
FT /note="Silicon efflux transporter LSI3"
FT /id="PRO_0000440949"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 233..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 485 AA; 51771 MW; F5935DE1A9B196BF CRC64;
MALASLPKVV MGSVAFGVFW MLAVFPSVPF LPIGRTAGAL LGAVLMIVFH VISADDAYAS
IDLPILGLLF ATMVVGGYLK NAGMFRHLGR LLAWRSQGGR DLMCRVCVVT ALASALFTND
TCCVVLTEFV LELAAERNLP AKPFLLALAT SANIGSSATP IGNPQNLVIA FNSKISFISF
LLGILPAMLA GMGINMLMLL CMYWKELDGG ACSPDEVAAG KQMEAIEEGR RTALNNNKKD
DGDAATPASP EDDDGGDAES MMSENISTKH RWFMQCSEHR RKLFLKSFAY VVTVGMLVAY
MLGLNMSWTA ITTAIALVVV DFRDAEPCLD KVSYSLLVFF SGMFVTVSGF NKTGLPGAIW
NVMAPYSKIN HVTGVTVLSV IILLLSNLAS NVPTVLLMGD EVAAAAATIS PAAVTRSWLL
LAWVSTVAGN LSLLGSAANL IVCEQARRAT RNAYDLTFWN HVIFGLPSTL VVTAIGIPLI
GKINI
