LSM12_HUMAN
ID LSM12_HUMAN Reviewed; 195 AA.
AC Q3MHD2; Q86YB1; Q96NL5;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Protein LSM12;
GN Name=LSM12;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-128 (ISOFORM 2), AND VARIANT ARG-77.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 2-18; 25-37 AND 73-86, CLEAVAGE OF INITIATOR
RP METHIONINE, ACETYLATION AT ALA-2, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RA Bienvenut W.V., Waridel P., Quadroni M.;
RL Submitted (MAR-2009) to UniProtKB.
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [11]
RP FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, MASS SPECTROMETRY, AND INTERACTION
RP WITH TPCN2.
RX PubMed=34362892; DOI=10.1038/s41467-021-24735-z;
RA Zhang J., Guan X., Shah K., Yan J.;
RT "Lsm12 is an NAADP receptor and a two-pore channel regulatory protein
RT required for calcium mobilization from acidic organelles.";
RL Nat. Commun. 12:4739-4739(2021).
CC -!- FUNCTION: Nicotinic acid adenine dinucleotide phosphate (NAADP) binding
CC protein (PubMed:34362892). Confers NAADP sensitivity to the two pore
CC channel complex (TPCs) by acting as TPC accessory protein necessary for
CC NAADP-evoked Ca(2+) release (PubMed:34362892).
CC {ECO:0000269|PubMed:34362892}.
CC -!- SUBUNIT: Found in a complex with LSM12, TPCN1 and TPCN2
CC (PubMed:34362892). Interacts with TPCN2 (PubMed:34362892).
CC {ECO:0000269|PubMed:34362892}.
CC -!- INTERACTION:
CC Q3MHD2; Q9Y333: LSM2; NbExp=3; IntAct=EBI-725133, EBI-347416;
CC Q3MHD2; P62310: LSM3; NbExp=3; IntAct=EBI-725133, EBI-348239;
CC Q3MHD2; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-725133, EBI-741158;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:34362892}.
CC Note=Colocalizes with TPCN2. {ECO:0000269|PubMed:34362892}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3MHD2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3MHD2-2; Sequence=VSP_028241;
CC -!- SIMILARITY: Belongs to the LSM12 family. {ECO:0000305}.
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DR EMBL; AK055218; BAB70877.1; -; mRNA.
DR EMBL; AC023855; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KF456277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC044587; AAH44587.1; -; mRNA.
DR EMBL; BC105288; AAI05289.1; -; mRNA.
DR CCDS; CCDS11475.1; -. [Q3MHD2-1]
DR RefSeq; NP_689557.1; NM_152344.3. [Q3MHD2-1]
DR AlphaFoldDB; Q3MHD2; -.
DR BioGRID; 125889; 136.
DR IntAct; Q3MHD2; 31.
DR MINT; Q3MHD2; -.
DR STRING; 9606.ENSP00000466718; -.
DR ChEMBL; CHEMBL4295839; -.
DR GlyGen; Q3MHD2; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q3MHD2; -.
DR PhosphoSitePlus; Q3MHD2; -.
DR BioMuta; LSM12; -.
DR DMDM; 158705881; -.
DR EPD; Q3MHD2; -.
DR jPOST; Q3MHD2; -.
DR MassIVE; Q3MHD2; -.
DR MaxQB; Q3MHD2; -.
DR PaxDb; Q3MHD2; -.
DR PeptideAtlas; Q3MHD2; -.
DR PRIDE; Q3MHD2; -.
DR ProteomicsDB; 61781; -. [Q3MHD2-1]
DR ProteomicsDB; 61782; -. [Q3MHD2-2]
DR Antibodypedia; 29665; 95 antibodies from 20 providers.
DR DNASU; 124801; -.
DR Ensembl; ENST00000293406.8; ENSP00000293406.3; ENSG00000161654.10. [Q3MHD2-1]
DR Ensembl; ENST00000591247.6; ENSP00000466718.1; ENSG00000161654.10. [Q3MHD2-1]
DR GeneID; 124801; -.
DR KEGG; hsa:124801; -.
DR MANE-Select; ENST00000293406.8; ENSP00000293406.3; NM_001371445.1; NP_001358374.1.
DR UCSC; uc002iev.3; human. [Q3MHD2-1]
DR CTD; 124801; -.
DR DisGeNET; 124801; -.
DR GeneCards; LSM12; -.
DR HGNC; HGNC:26407; LSM12.
DR HPA; ENSG00000161654; Low tissue specificity.
DR MIM; 611793; gene.
DR neXtProt; NX_Q3MHD2; -.
DR OpenTargets; ENSG00000161654; -.
DR PharmGKB; PA142671502; -.
DR VEuPathDB; HostDB:ENSG00000161654; -.
DR eggNOG; KOG4401; Eukaryota.
DR GeneTree; ENSGT00390000006956; -.
DR HOGENOM; CLU_073383_3_0_1; -.
DR InParanoid; Q3MHD2; -.
DR OMA; WEGANIR; -.
DR OrthoDB; 1601482at2759; -.
DR PhylomeDB; Q3MHD2; -.
DR PathwayCommons; Q3MHD2; -.
DR SignaLink; Q3MHD2; -.
DR BioGRID-ORCS; 124801; 232 hits in 1079 CRISPR screens.
DR ChiTaRS; LSM12; human.
DR GenomeRNAi; 124801; -.
DR Pharos; Q3MHD2; Tbio.
DR PRO; PR:Q3MHD2; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q3MHD2; protein.
DR Bgee; ENSG00000161654; Expressed in gingival epithelium and 204 other tissues.
DR ExpressionAtlas; Q3MHD2; baseline and differential.
DR Genevisible; Q3MHD2; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR InterPro; IPR019181; Anticodon-binding_dom.
DR InterPro; IPR039683; Lsm12-like.
DR PANTHER; PTHR13542; PTHR13542; 1.
DR Pfam; PF09793; AD; 1.
DR SMART; SM00995; AD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Direct protein sequencing;
KW Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.4, ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT CHAIN 2..195
FT /note="Protein LSM12"
FT /id="PRO_0000305126"
FT REGION 4..67
FT /note="Required for NAADP and TPCN2 binding"
FT /evidence="ECO:0000269|PubMed:34362892"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|Ref.4, ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT MOD_RES 75
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"
FT VAR_SEQ 42
FT /note="K -> ISPE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028241"
FT VARIANT 77
FT /note="P -> R (in dbSNP:rs17854322)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_035166"
FT VARIANT 173
FT /note="V -> L (in dbSNP:rs59168537)"
FT /id="VAR_062209"
SQ SEQUENCE 195 AA; 21701 MW; BCEDE62DA43D86C7 CRC64;
MAAPPGEYFS VGSQVSCRTC QEQRLQGEVV AFDYQSKMLA LKCPSSSGKP NHADILLINL
QYVSEVEIIN DRTETPPPLA SLNVSKLASK ARTEKEEKLS QAYAISAGVS LEGQQLFQTI
HKTIKDCKWQ EKNIVVMEEV VITPPYQVEN CKGKEGSALS HVRKIVEKHF RDVESQKILQ
RSQAQQPQKE AALSS
