LSM12_MOUSE
ID LSM12_MOUSE Reviewed; 195 AA.
AC Q9D0R8; A2AWS3;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein LSM12;
GN Name=Lsm12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-75, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP FUNCTION.
RX PubMed=34362892; DOI=10.1038/s41467-021-24735-z;
RA Zhang J., Guan X., Shah K., Yan J.;
RT "Lsm12 is an NAADP receptor and a two-pore channel regulatory protein
RT required for calcium mobilization from acidic organelles.";
RL Nat. Commun. 12:4739-4739(2021).
CC -!- FUNCTION: Nicotinic acid adenine dinucleotide phosphate (NAADP) binding
CC protein. Confers NAADP sensitivity to the two pore channel complex
CC (TPCs) by acting as TPC accessory protein necessary for NAADP-evoked
CC Ca(2+) release. {ECO:0000250|UniProtKB:Q3MHD2}.
CC -!- SUBUNIT: Found in a complex with LSM12, TPCN1 and TPCN2. Interacts with
CC TPCN2. {ECO:0000250|UniProtKB:Q3MHD2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q3MHD2}.
CC Note=Colocalizes with TPCN2. {ECO:0000250|UniProtKB:Q3MHD2}.
CC -!- SIMILARITY: Belongs to the LSM12 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAM16077.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM25069.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AK011126; BAB27419.1; -; mRNA.
DR EMBL; AK075668; BAC35885.1; -; mRNA.
DR EMBL; AK132889; BAE21407.1; -; mRNA.
DR EMBL; AK151118; BAE30127.1; -; mRNA.
DR EMBL; BX323852; CAM16076.1; -; Genomic_DNA.
DR EMBL; AL954730; CAM16076.1; JOINED; Genomic_DNA.
DR EMBL; BX323852; CAM16077.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL954730; CAM16077.1; JOINED; Genomic_DNA.
DR EMBL; AL954730; CAM25068.1; -; Genomic_DNA.
DR EMBL; BX323852; CAM25068.1; JOINED; Genomic_DNA.
DR EMBL; AL954730; CAM25069.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BX323852; CAM25069.1; JOINED; Genomic_DNA.
DR EMBL; BC027784; AAH27784.1; -; mRNA.
DR CCDS; CCDS25490.1; -.
DR RefSeq; NP_766535.1; NM_172947.3.
DR RefSeq; XP_006533477.1; XM_006533414.3.
DR AlphaFoldDB; Q9D0R8; -.
DR BioGRID; 234509; 12.
DR IntAct; Q9D0R8; 1.
DR MINT; Q9D0R8; -.
DR STRING; 10090.ENSMUSP00000021297; -.
DR iPTMnet; Q9D0R8; -.
DR PhosphoSitePlus; Q9D0R8; -.
DR EPD; Q9D0R8; -.
DR jPOST; Q9D0R8; -.
DR MaxQB; Q9D0R8; -.
DR PaxDb; Q9D0R8; -.
DR PeptideAtlas; Q9D0R8; -.
DR PRIDE; Q9D0R8; -.
DR ProteomicsDB; 292045; -.
DR Antibodypedia; 29665; 95 antibodies from 20 providers.
DR DNASU; 268490; -.
DR Ensembl; ENSMUST00000021297; ENSMUSP00000021297; ENSMUSG00000020922.
DR GeneID; 268490; -.
DR KEGG; mmu:268490; -.
DR UCSC; uc007lqs.1; mouse.
DR CTD; 124801; -.
DR MGI; MGI:1919592; Lsm12.
DR VEuPathDB; HostDB:ENSMUSG00000020922; -.
DR eggNOG; KOG4401; Eukaryota.
DR GeneTree; ENSGT00390000006956; -.
DR HOGENOM; CLU_073383_3_0_1; -.
DR InParanoid; Q9D0R8; -.
DR OMA; WEGANIR; -.
DR OrthoDB; 1601482at2759; -.
DR PhylomeDB; Q9D0R8; -.
DR TreeFam; TF324296; -.
DR BioGRID-ORCS; 268490; 12 hits in 73 CRISPR screens.
DR ChiTaRS; Lsm12; mouse.
DR PRO; PR:Q9D0R8; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9D0R8; protein.
DR Bgee; ENSMUSG00000020922; Expressed in interventricular septum and 239 other tissues.
DR ExpressionAtlas; Q9D0R8; baseline and differential.
DR Genevisible; Q9D0R8; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR InterPro; IPR019181; Anticodon-binding_dom.
DR InterPro; IPR039683; Lsm12-like.
DR PANTHER; PTHR13542; PTHR13542; 1.
DR Pfam; PF09793; AD; 1.
DR SMART; SM00995; AD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q3MHD2"
FT CHAIN 2..195
FT /note="Protein LSM12"
FT /id="PRO_0000305127"
FT REGION 4..67
FT /note="Required for NAADP and TPCN2 binding"
FT /evidence="ECO:0000250|UniProtKB:Q3MHD2"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q3MHD2"
FT MOD_RES 75
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 195 AA; 21701 MW; BCEDE62DA43D86C7 CRC64;
MAAPPGEYFS VGSQVSCRTC QEQRLQGEVV AFDYQSKMLA LKCPSSSGKP NHADILLINL
QYVSEVEIIN DRTETPPPLA SLNVSKLASK ARTEKEEKLS QAYAISAGVS LEGQQLFQTI
HKTIKDCKWQ EKNIVVMEEV VITPPYQVEN CKGKEGSALS HVRKIVEKHF RDVESQKILQ
RSQAQQPQKE AALSS
