LSM2_MOUSE
ID LSM2_MOUSE Reviewed; 95 AA.
AC O35900;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=U6 snRNA-associated Sm-like protein LSm2;
DE AltName: Full=Protein G7b;
DE AltName: Full=snRNP core Sm-like protein Sm-x5;
GN Name=Lsm2; Synonyms=G7b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BALB/cJ;
RX PubMed=9271633; DOI=10.1007/s002510050297;
RA Bedian V., Adams T., Geiger E.A., Bailey L.C., Gasser D.L.;
RT "A gene belonging to the Sm family of snRNP core proteins maps within the
RT mouse MHC.";
RL Immunogenetics 46:427-430(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B10.A;
RX PubMed=11797099; DOI=10.1007/s00251-001-0381-0;
RA van Kooij M., de Groot K., van Vugt H., Aten J., Snoek M.;
RT "Genotype versus phenotype: conflicting results in mapping a lung tumor
RT susceptibility locus to the G7c recombination interval in the mouse MHC
RT class III region.";
RL Immunogenetics 53:656-661(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays role in pre-mRNA splicing as component of the U4/U6-U5
CC tri-snRNP complex that is involved in spliceosome assembly, and as
CC component of the precatalytic spliceosome (spliceosome B complex). The
CC heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract
CC of U6 snRNA. {ECO:0000250|UniProtKB:Q9Y333}.
CC -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC complex). Component of the U4/U6-U5 tri-snRNP complex, a building block
CC of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5
CC tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least
CC PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB,
CC SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH,
CC SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7
CC and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a
CC heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of
CC the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.
CC {ECO:0000250|UniProtKB:Q9Y333}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y333}.
CC -!- SIMILARITY: Belongs to the snRNP Sm proteins family. {ECO:0000305}.
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DR EMBL; U85207; AAB72037.1; -; Genomic_DNA.
DR EMBL; AF397035; AAL14450.1; -; Genomic_DNA.
DR EMBL; AF397036; AAL14458.1; -; Genomic_DNA.
DR EMBL; BC014288; AAH14288.1; -; mRNA.
DR CCDS; CCDS50081.1; -.
DR RefSeq; NP_001103571.1; NM_001110101.2.
DR AlphaFoldDB; O35900; -.
DR SMR; O35900; -.
DR BioGRID; 205506; 33.
DR STRING; 10090.ENSMUSP00000007266; -.
DR iPTMnet; O35900; -.
DR PhosphoSitePlus; O35900; -.
DR REPRODUCTION-2DPAGE; O35900; -.
DR EPD; O35900; -.
DR jPOST; O35900; -.
DR PaxDb; O35900; -.
DR PeptideAtlas; O35900; -.
DR PRIDE; O35900; -.
DR ProteomicsDB; 292047; -.
DR Antibodypedia; 27800; 201 antibodies from 34 providers.
DR DNASU; 27756; -.
DR Ensembl; ENSMUST00000114011; ENSMUSP00000109644; ENSMUSG00000007050.
DR GeneID; 27756; -.
DR KEGG; mmu:27756; -.
DR UCSC; uc008cer.3; mouse.
DR CTD; 57819; -.
DR MGI; MGI:90676; Lsm2.
DR VEuPathDB; HostDB:ENSMUSG00000007050; -.
DR eggNOG; KOG3448; Eukaryota.
DR GeneTree; ENSGT00390000016597; -.
DR HOGENOM; CLU_130474_3_0_1; -.
DR InParanoid; O35900; -.
DR OMA; EERYPHM; -.
DR PhylomeDB; O35900; -.
DR Reactome; R-MMU-430039; mRNA decay by 5' to 3' exoribonuclease.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR BioGRID-ORCS; 27756; 28 hits in 73 CRISPR screens.
DR ChiTaRS; Lsm2; mouse.
DR PRO; PR:O35900; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; O35900; protein.
DR Bgee; ENSMUSG00000007050; Expressed in undifferentiated genital tubercle and 194 other tissues.
DR ExpressionAtlas; O35900; baseline and differential.
DR Genevisible; O35900; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:1990726; C:Lsm1-7-Pat1 complex; IBA:GO_Central.
DR GO; GO:0120115; C:Lsm2-8 complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISS:UniProtKB.
DR GO; GO:0005688; C:U6 snRNP; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IDA:MGI.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR GO; GO:0006402; P:mRNA catabolic process; ISO:MGI.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; ISO:MGI.
DR CDD; cd01725; LSm2; 1.
DR InterPro; IPR001163; LSM_dom_euk/arc.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR016654; U6_snRNA_Lsm2.
DR PANTHER; PTHR13829; PTHR13829; 1.
DR Pfam; PF01423; LSM; 1.
DR PIRSF; PIRSF016394; U6_snRNA_Lsm2; 1.
DR SMART; SM00651; Sm; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..95
FT /note="U6 snRNA-associated Sm-like protein LSm2"
FT /id="PRO_0000125557"
FT MOD_RES 79
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y333"
SQ SEQUENCE 95 AA; 10835 MW; 623591A09A6ABACE CRC64;
MLFYSFFKSL VGKDVVVELK NDLSICGTLH SVDQYLNIKL TDISVTDPEK YPHMLSVKNC
FIRGSVVRYV QLPADEVDTQ LLQDAARKEA LQQKQ
