LSP1G_DROME
ID LSP1G_DROME Reviewed; 772 AA.
AC P11997; O16162; Q9W0V6;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Larval serum protein 1 gamma chain;
DE AltName: Full=Hexamerin-1-gamma;
DE Flags: Precursor;
GN Name=Lsp1gamma; Synonyms=Lsp1-g; ORFNames=CG6821;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-711.
RX PubMed=9402735; DOI=10.1093/oxfordjournals.molbev.a025734;
RA Bauer V.L., Aquadro C.F.;
RT "Rates of DNA sequence evolution are not sex-biased in Drosophila
RT melanogaster and D. simulans.";
RL Mol. Biol. Evol. 14:1252-1257(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-105, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=3097321; DOI=10.1016/0022-2836(86)90376-1;
RA Delaney S.J., Smith D.F., McClelland A., Sunkel C., Glover D.M.;
RT "Sequence conservation around the 5' ends of the larval serum protein 1
RT genes of Drosophila melanogaster.";
RL J. Mol. Biol. 189:1-11(1986).
CC -!- FUNCTION: Larval storage protein (LSP) which may serve as a store of
CC amino acids for synthesis of adult proteins. {ECO:0000250}.
CC -!- SUBUNIT: Heterohexamer, composed of three subunits, alpha, beta and
CC gamma.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC {ECO:0000269|PubMed:3097321}.
CC -!- TISSUE SPECIFICITY: Larval hemolymph. {ECO:0000269|PubMed:3097321}.
CC -!- SIMILARITY: Belongs to the hemocyanin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA27508.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF47324.1; -; Genomic_DNA.
DR EMBL; AY069748; AAL39893.1; -; mRNA.
DR EMBL; AF016033; AAB71666.1; -; Genomic_DNA.
DR EMBL; X03874; CAA27508.1; ALT_FRAME; Genomic_DNA.
DR PIR; C27144; C27144.
DR RefSeq; NP_523868.1; NM_079144.5.
DR AlphaFoldDB; P11997; -.
DR SMR; P11997; -.
DR BioGRID; 63580; 2.
DR STRING; 7227.FBpp0072365; -.
DR GlyGen; P11997; 1 site.
DR PaxDb; P11997; -.
DR PRIDE; P11997; -.
DR DNASU; 38015; -.
DR EnsemblMetazoa; FBtr0072463; FBpp0072365; FBgn0002564.
DR GeneID; 38015; -.
DR KEGG; dme:Dmel_CG6821; -.
DR CTD; 38015; -.
DR FlyBase; FBgn0002564; Lsp1gamma.
DR VEuPathDB; VectorBase:FBgn0002564; -.
DR eggNOG; ENOG502QR98; Eukaryota.
DR GeneTree; ENSGT00940000165243; -.
DR HOGENOM; CLU_012213_1_0_1; -.
DR InParanoid; P11997; -.
DR OMA; EYGRVIP; -.
DR OrthoDB; 244455at2759; -.
DR PhylomeDB; P11997; -.
DR BioGRID-ORCS; 38015; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Lsp1gamma; fly.
DR GenomeRNAi; 38015; -.
DR PRO; PR:P11997; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002564; Expressed in arthropod fat body and 13 other tissues.
DR ExpressionAtlas; P11997; baseline and differential.
DR Genevisible; P11997; DM.
DR GO; GO:0005616; C:larval serum protein complex; IDA:FlyBase.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1280.10; -; 1.
DR Gene3D; 1.20.1370.10; -; 1.
DR Gene3D; 2.60.40.1520; -; 1.
DR InterPro; IPR008922; Di-copper_centre_dom_sf.
DR InterPro; IPR013788; Hemocyanin/hexamerin.
DR InterPro; IPR000896; Hemocyanin/hexamerin_mid_dom.
DR InterPro; IPR005203; Hemocyanin_C.
DR InterPro; IPR037020; Hemocyanin_C_sf.
DR InterPro; IPR005204; Hemocyanin_N.
DR InterPro; IPR036697; Hemocyanin_N_sf.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR11511; PTHR11511; 1.
DR Pfam; PF03723; Hemocyanin_C; 1.
DR Pfam; PF00372; Hemocyanin_M; 1.
DR Pfam; PF03722; Hemocyanin_N; 1.
DR PRINTS; PR00187; HAEMOCYANIN.
DR SUPFAM; SSF48050; SSF48050; 1.
DR SUPFAM; SSF48056; SSF48056; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS00210; HEMOCYANIN_2; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Reference proteome; Secreted; Signal; Storage protein.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..772
FT /note="Larval serum protein 1 gamma chain"
FT /id="PRO_0000013335"
FT CARBOHYD 242
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 326..328
FT /note="QQI -> SRS (in Ref. 4; AAB71666)"
FT /evidence="ECO:0000305"
FT CONFLICT 624
FT /note="S -> T (in Ref. 4; AAB71666)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 772 AA; 93408 MW; FC2BC0A7336F636D CRC64;
MKLTLVILAL VACVTAFSVP TQKVKIADKN FLEKQKFLFE IVHRIDEPLM FEEWIKMGQK
LITDKAQYET FDFYMEKLWE SYKLGALLPK GEFFGALVKT HHKQAYGLFN FFYYAKDWET
FVRNVAWARI HVNEGMFVYA LTLAVIHKPE FEGLILPQIY EIFPQYFFNS KFVYAAEKFD
YEVFSKLTMY EKEYKDILYK DYSEFTGNFY FYTKDWKTWQ WYKMMGLDQE WYVEDKYFLR
ENLSQFVNDP KYVDVVKGLK KFYMPVDYTR DIDFFNDETK MTYFTEDLGW NAYWYYLNMD
YAFFLNGKQF GLDKDRRGEY WIYNVQQILA RYYQERLANG FGEIPEFFWY KQIEYGYDPQ
LIYYNGIGYS YRKNYYDFYT YGKFEMYSQI QNFFSRVYKV LETGFYKTAD GQVFDLHKPE
AIKIVGNYLQ GNADTFDKYF FNYYYLLAHM YFADVDYNDM EVFPNVFLNF ETMLRDPFFY
TFYKKFTDVF YTFKYYLKPY TQKDLFYEGI TIKDVSVSKL VTYYDIVDFD VTNLLNDKMT
FVDGQYIWDK ALLARQARLN HKPFNFEFTI DSDKVQKGVV RVFLGPKFDE YGRVIPLDYN
RKNFVQIDSF VYPFIAGTNT IKRSSKEFSW TAEDRITYTE LYKYVMLASE GKYDFPLDIS
EPHNAFPDRL VLPKGWEQGM PMQFYFFVSP FAETYEQFSN FDYTYSSGVG SGTRFVDTKP
FGYPFDRQID ESDFFVPNGF FKDVKVYYVD TFAKYFEKKY TQFGTFDYSI EY
