LSP2_DROME
ID LSP2_DROME Reviewed; 701 AA.
AC Q24388; Q9VTT8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Larval serum protein 2;
DE Short=LSP-2;
DE AltName: Full=Hexamerin-2;
DE Flags: Precursor;
GN Name=Lsp2; ORFNames=CG6806;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=9128741; DOI=10.1111/j.1432-1033.1997.00191.x;
RA Mousseron-Grall S., Kejzlarova-Lepesant J., Burmester T., Chihara C.,
RA Barray M., Delain E., Pictet R., Lepesant J.-A.;
RT "Sequence, structure and evolution of the ecdysone-inducible Lsp-2 gene of
RT Drosophila melanogaster.";
RL Eur. J. Biochem. 245:191-198(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Larval storage protein (LSP) which may serve as a store of
CC amino acids for synthesis of adult proteins. {ECO:0000250}.
CC -!- SUBUNIT: Homohexamer.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC -!- SIMILARITY: Belongs to the hemocyanin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA66371.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X97770; CAA66371.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE014296; AAF49958.1; -; Genomic_DNA.
DR EMBL; AY119005; AAM50865.1; -; mRNA.
DR RefSeq; NP_001287042.1; NM_001300113.1.
DR RefSeq; NP_524816.1; NM_080077.2.
DR AlphaFoldDB; Q24388; -.
DR SMR; Q24388; -.
DR BioGRID; 69593; 30.
DR IntAct; Q24388; 1.
DR STRING; 7227.FBpp0088363; -.
DR GlyGen; Q24388; 1 site.
DR PaxDb; Q24388; -.
DR DNASU; 45326; -.
DR EnsemblMetazoa; FBtr0089324; FBpp0088363; FBgn0002565.
DR EnsemblMetazoa; FBtr0345352; FBpp0311507; FBgn0002565.
DR GeneID; 45326; -.
DR KEGG; dme:Dmel_CG6806; -.
DR CTD; 45326; -.
DR FlyBase; FBgn0002565; Lsp2.
DR VEuPathDB; VectorBase:FBgn0002565; -.
DR eggNOG; ENOG502QR98; Eukaryota.
DR HOGENOM; CLU_012213_1_0_1; -.
DR InParanoid; Q24388; -.
DR OMA; NHIKRAS; -.
DR OrthoDB; 244455at2759; -.
DR PhylomeDB; Q24388; -.
DR SignaLink; Q24388; -.
DR BioGRID-ORCS; 45326; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Lsp2; fly.
DR GenomeRNAi; 45326; -.
DR PRO; PR:Q24388; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002565; Expressed in arthropod fat body and 19 other tissues.
DR ExpressionAtlas; Q24388; baseline and differential.
DR Genevisible; Q24388; DM.
DR GO; GO:0005616; C:larval serum protein complex; IDA:FlyBase.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:FlyBase.
DR GO; GO:0016201; P:synaptic target inhibition; IMP:FlyBase.
DR Gene3D; 1.10.1280.10; -; 1.
DR Gene3D; 1.20.1370.10; -; 1.
DR Gene3D; 2.60.40.1520; -; 1.
DR InterPro; IPR008922; Di-copper_centre_dom_sf.
DR InterPro; IPR013788; Hemocyanin/hexamerin.
DR InterPro; IPR000896; Hemocyanin/hexamerin_mid_dom.
DR InterPro; IPR005203; Hemocyanin_C.
DR InterPro; IPR037020; Hemocyanin_C_sf.
DR InterPro; IPR005204; Hemocyanin_N.
DR InterPro; IPR036697; Hemocyanin_N_sf.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR11511; PTHR11511; 1.
DR Pfam; PF03723; Hemocyanin_C; 1.
DR Pfam; PF00372; Hemocyanin_M; 1.
DR Pfam; PF03722; Hemocyanin_N; 1.
DR PRINTS; PR00187; HAEMOCYANIN.
DR SUPFAM; SSF48050; SSF48050; 1.
DR SUPFAM; SSF48056; SSF48056; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS00210; HEMOCYANIN_2; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Reference proteome; Secreted; Signal; Storage protein.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..701
FT /note="Larval serum protein 2"
FT /id="PRO_0000013336"
FT CARBOHYD 204
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 271
FT /note="Y -> H (in Ref. 1; CAA66371)"
FT /evidence="ECO:0000305"
FT CONFLICT 335
FT /note="V -> L (in Ref. 1; CAA66371)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 701 AA; 83349 MW; 4CC348AC7C05138D CRC64;
MKSFTVIALA AVALLATLGQ AKHLDSKVAD KDFLMKQKFM YQILQHIYQD DVFTTPFGGS
YVEYKPWEHV ADYVHPEMLE HFFELWQHQP FTDDMVWSVM YDKHEEYVVG LVRLFYFAKN
WETFQHVVYW ARQHVNKQLF VYAVTIASLF RDDMQGVVLP AHYEIHPWSY FDSQALEWAE
HYKMHGFHHV KQMDNIYNVV IRTNYSNVHG SLNYDHDLAY YLEDVGFNAF YYYFNLDYPF
WTKGGEEHVL NKDRRGELYL YVHWQLLARW YLERLSHDLG EVPAFNMYVP TESGYASNLR
TYYGVPQWHR ENHHSFYHEH NYEHIEHVEM YTQRVMDWIH KNEKFDVETI NVLGNIIQGN
ADSVDKKFYG SLDKLYRFIV NEGHHYGHGD ESFPGLFMHY DTSMRDPIFY EVYKTIVSHY
WHLMETYPEY HKKDYAFEGV HIDAVHMPES LTTYFEHFDS DISNAVNVEP AVEGSADPLY
TFGRNSHYKG SSYVIKARQQ RLNHKPFEFT LDVTSDKAQD AVVKVFIGPK YDEHGHEIPL
EHNYQNFFEL EHFKVHLEAG VNHIKRASGD FSFWVNDRTT YLELYQKLMD ATNSDYKFKL
DQSEAHCGVP NRMMLPRGKK GGQVFQFFYM VYPYHQPEVA QFTGYDPVVS CGVGHGSRYV
DALPFGFPFN RPVKHDYYFD VHNFKFVDVK IFHRDEHTNV V
