5MAT1_SALSN
ID 5MAT1_SALSN Reviewed; 462 AA.
AC Q8W1W9;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Malonyl-coenzyme:anthocyanin 5-O-glucoside-6'''-O-malonyltransferase {ECO:0000303|PubMed:11598135};
DE Short=Malonyl CoA:anthocyanin 5-O-glucoside-6'''-O-malonyltransferase {ECO:0000312|EMBL:AAL50566.1};
DE Short=Ss5MaT1 {ECO:0000303|PubMed:11598135};
DE EC=2.3.1.172;
GN Name=5MAT1 {ECO:0000303|PubMed:11598135};
OS Salvia splendens (Scarlet sage).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salviinae;
OC Salvia; Salvia subgen. Calosphace; core Calosphace.
OX NCBI_TaxID=180675;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAL50566.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ACTIVITY
RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Petal {ECO:0000269|PubMed:11598135};
RX PubMed=11598135; DOI=10.1074/jbc.m108444200;
RA Suzuki H., Nakayama T., Yonekura-Sakakibara K., Fukui Y., Nakamura N.,
RA Nakao M., Tanaka Y., Yamaguchi M.A., Kusumi T., Nishino T.;
RT "Malonyl-CoA:anthocyanin 5-O-glucoside-6'''-O-malonyltransferase from
RT scarlet sage (Salvia splendens) flowers. Enzyme purification, gene cloning,
RT expression, and characterization.";
RL J. Biol. Chem. 276:49013-49019(2001).
RN [2] {ECO:0000305}
RP MUTAGENESIS OF TYR-45; GLN-150; HIS-166; HIS-167; CYS-168; ASP-171;
RP LYS-258; ARG-301; ASN-315 AND ASP-390, PROPOSED ENZYME MECHANISM, AND
RP ACTIVE SITES.
RX PubMed=12578391; DOI=10.1021/bi020618g;
RA Suzuki H., Nakayama T., Nishino T.;
RT "Proposed mechanism and functional amino acid residues of malonyl-
RT CoA:anthocyanin 5-O-glucoside-6'''-O-malonyltransferase from flowers of
RT Salvia splendens, a member of the versatile plant acyltransferase family.";
RL Biochemistry 42:1764-1771(2003).
CC -!- FUNCTION: Catalyzes the transfer of a malonyl group from malonyl-CoA to
CC the 6'''-hydroxyl group of the 5-glucosyl moiety of anthocyanins.
CC Active towards bisdemalonylsalvianin (pelargonidin 3-O-(6-caffeoyl-
CC beta-D-glucoside) 5-O-beta-D-glucoside) and shisonin, but not towards
CC nodemalonylsalvianin, salvianin, pelargonidin 3,5-diglucoside and
CC delphinidin 3,5-diglucoside. {ECO:0000269|PubMed:11598135}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=malonyl-CoA + pelargonidin 3-O-(6-O-[(E)-caffeoyl]-beta-D-
CC glucoside) 5-O-beta-D-glucoside = 4'''-demalonylsalvianin + CoA;
CC Xref=Rhea:RHEA:21988, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384,
CC ChEBI:CHEBI:58638, ChEBI:CHEBI:58640; EC=2.3.1.172;
CC Evidence={ECO:0000269|PubMed:11598135};
CC -!- ACTIVITY REGULATION: Completely inhibited by 10 mM p-coumaric acid,
CC this inhibition is rapid, reversible and non-competitive. Completely
CC inhibited by 0.1 mM Cu(2+), 0.1 mM Hg(2+) and 10 mM caffeic acid.
CC Partially inhibited by 5 mM N-ethylmaleimide, 1 mM diethylpyrocarbonate
CC and 1 mM acetyl-CoA. {ECO:0000269|PubMed:11598135}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=101.0 uM for bisdemalonylsalvianin (with malonyl-CoA as
CC cosubstrate) {ECO:0000269|PubMed:11598135};
CC KM=40.2 uM for shisonin (with malonyl-CoA as cosubstrate)
CC {ECO:0000269|PubMed:11598135};
CC KM=57.0 uM for malonyl-CoA (with bisdemalonylsalvianin as
CC cosubstrate) {ECO:0000269|PubMed:11598135};
CC KM=14.1 uM for malonyl-CoA (with shisonin as cosubstrate)
CC {ECO:0000269|PubMed:11598135};
CC pH dependence:
CC Optimum pH is 8.0. Active over the pH range 6.0-11.0. Stable at pH
CC 7.5 for 20 hours at 20 degrees Celsius.
CC {ECO:0000269|PubMed:11598135};
CC Temperature dependence:
CC Stable below 30 degrees Celsius for 20 minutes at pH 7.0.
CC {ECO:0000269|PubMed:11598135};
CC -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC -!- TISSUE SPECIFICITY: Detected in petals and sepals, and at lower levels
CC in bracts and red stems. {ECO:0000269|PubMed:11598135}.
CC -!- DEVELOPMENTAL STAGE: Highest levels are present in the petals and
CC sepals of young to opening flowers. {ECO:0000269|PubMed:11598135}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000255}.
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DR EMBL; AF405707; AAL50566.1; -; mRNA.
DR AlphaFoldDB; Q8W1W9; -.
DR SMR; Q8W1W9; -.
DR KEGG; ag:AAL50566; -.
DR BRENDA; 2.3.1.172; 7433.
DR SABIO-RK; Q8W1W9; -.
DR UniPathway; UPA00009; -.
DR GO; GO:0033810; F:anthocyanin 5-O-glucoside 6'''-O-malonyltransferase activity; IDA:UniProtKB.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:UniProtKB.
DR GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Phenylpropanoid metabolism; Transferase.
FT CHAIN 1..462
FT /note="Malonyl-coenzyme:anthocyanin 5-O-glucoside-6'''-O-
FT malonyltransferase"
FT /id="PRO_0000405127"
FT ACT_SITE 167
FT /note="Proton acceptor"
FT /evidence="ECO:0000305|PubMed:12578391"
FT ACT_SITE 390
FT /note="Proton acceptor"
FT /evidence="ECO:0000305|PubMed:12578391"
FT MUTAGEN 45
FT /note="Y->A: Activity reduced to 11% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 150
FT /note="Q->A: Activity reduced to 67% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 166
FT /note="H->A: Activity reduced to 23% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 167
FT /note="H->A: Activity reduced to 0.02% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 168
FT /note="C->A: Activity reduced to 8% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 171
FT /note="D->A: Activity reduced to 0.8% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 258
FT /note="K->A: Activity reduced to 61% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 301
FT /note="R->A: Activity reduced to 17% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 315
FT /note="N->A: Activity reduced to 1.1% of wild-type."
FT /evidence="ECO:0000269|PubMed:12578391"
FT MUTAGEN 390
FT /note="D->A: Activity abolished."
FT /evidence="ECO:0000269|PubMed:12578391"
SQ SEQUENCE 462 AA; 50724 MW; 220B4853BC94DB2F CRC64;
MTTTTTILET CHIPPPPAAN DLSIPLSFFD IKWLHYHPVR RLLFYHHPSS KSQFLHTIVP
HLKQSLSLAL THYLPVAGNL LYPSNTEKFP QLRYAAGDSV PVTIAESNSD FESLTGNHTR
DADQFYDLLP PIPPIEEESD WKLINIFAVQ ITLFPGEGIC IGFSNHHCLG DARSIVGFIS
AWGEINGIGG YEGFLSNHSD SLSLPIFDRS FINDPNKIDA IFWKVLRNIP LKTASFPLPT
NRVRSTFLLR RSDIEKLKTA TKSPASSFVA AAAFVWSCMV KSGDKSDENA PELFIIPADA
RGRVDPPIPE NYFGNCIVSS VAQVERGKLA AEDGFAVAAE AIGGEIEGKL KNRDEILRGA
ENWMSDIFKC FGMSVLGVSG SPKFDLLKAD FGWGKARKLE VLSIDGENHS MSLCSSSDFN
GGLEVGLSLP RERMAAFEEV FRASIMAASG PARRSPALVE PL
