ID   LSRA_SALTY              Reviewed;         511 AA.
AC   Q8ZKQ4;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Autoinducer 2 import ATP-binding protein LsrA;
DE            Short=AI-2 import ATP-binding protein LsrA;
DE            EC=7.6.2.13 {ECO:0000305|PubMed:11722742};
GN   Name=lsrA; OrderedLocusNames=STM4074;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   FUNCTION IN AI-2 IMPORT, AND INDUCTION.
RC   STRAIN=ATCC 14028 / SGSG 2980 / CDC 6516-60 / NCTC 12023;
RX   PubMed=11722742; DOI=10.1046/j.1365-2958.2001.02669.x;
RA   Taga M.E., Semmelhack J.L., Bassler B.L.;
RT   "The LuxS-dependent autoinducer AI-2 controls the expression of an ABC
RT   transporter that functions in AI-2 uptake in Salmonella typhimurium.";
RL   Mol. Microbiol. 42:777-793(2001).
CC   -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC       autoinducer 2 (AI-2) import. Responsible for energy coupling to the
CC       transport system. {ECO:0000305|PubMed:11722742}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + (2R,4S)-2-methyl-2,3,3,4-
CC         tetrahydroxytetrahydrofuran-[AI-2-binding protein]Side 1 = ADP +
CC         phosphate + (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuranSide
CC         2 + [AI-2-binding protein]Side 1.; EC=7.6.2.13;
CC         Evidence={ECO:0000305|PubMed:11722742};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC       two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC       (LsrB). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Peripheral
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: In the absence of AI-2, repressed by LsrR. Induced by AI-2,
CC       via release of the LsrR repressor. {ECO:0000269|PubMed:11722742}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. AI-2
CC       autoinducer porter (TC 3.A.1.2.8) family. {ECO:0000305}.
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DR   EMBL; AE006468; AAL22914.1; -; Genomic_DNA.
DR   RefSeq; NP_462955.1; NC_003197.2.
DR   RefSeq; WP_001167250.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZKQ4; -.
DR   SMR; Q8ZKQ4; -.
DR   STRING; 99287.STM4074; -.
DR   PaxDb; Q8ZKQ4; -.
DR   DNASU; 1255601; -.
DR   EnsemblBacteria; AAL22914; AAL22914; STM4074.
DR   GeneID; 1255601; -.
DR   KEGG; stm:STM4074; -.
DR   PATRIC; fig|99287.12.peg.4294; -.
DR   HOGENOM; CLU_000604_92_3_6; -.
DR   OMA; ITHRFPE; -.
DR   PhylomeDB; Q8ZKQ4; -.
DR   BioCyc; SENT99287:STM4074-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:1905887; P:autoinducer AI-2 transmembrane transport; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR030281; LsrA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR43790:SF2; PTHR43790:SF2; 1.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Repeat; Translocase; Transport.
FT   CHAIN           1..511
FT                   /note="Autoinducer 2 import ATP-binding protein LsrA"
FT                   /id="PRO_0000351304"
FT   DOMAIN          12..240
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   DOMAIN          263..503
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         44..51
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   511 AA;  55517 MW;  AA1D88B327104909 CRC64;
     MQISHNTASP LICVQNIYKS YSGVEVLKGI DFTLHAGEVH ALLGGNGAGK STLMKIIAGI
     VPPDGGTIDI AGVRCSHLTP LKAHQYGIYL VPQEPLLFPS LSVRENILFG LQGRQASTEK
     MQQLLKAMGC QLDPASAAGT LDVADRQIVE IMRGLMRDSR ILILDEPTAS LTPAETDRLF
     TRLQELLKKG VGIVFISHKL PEIRQLAHCV SVMRDGKIAL FGKTHDLSTD EIIQAITPAT
     QGVSLSANQK LWLELPGSRP QNERGATVLA LESLTGEGFM NINLEVRAGE ILGLAGLVGA
     GRTELAETLY GIRPVNAGRM LFNGQEINAL TTQQRLQLGL VYLPEDRQSS GLYLDASLAW
     NVCSLTHNQK GFWIKPQRDN ATLERYHRAL NIKLNNAEQA ARTLSGGNQQ KVLIAKCLEA
     SPQLLIVDEP TRGVDVSARS DIYQLLRSIA QQNVAVLFIS SDLEEIEQMA DRVYVMHQGE
     LGGPALCGEE INVDTIMHVA FGEHGASEAT C