LSRA_SHIFL
ID LSRA_SHIFL Reviewed; 511 AA.
AC Q83L12; Q7C1J2;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Autoinducer 2 import ATP-binding protein LsrA {ECO:0000250|UniProtKB:P77257};
DE Short=AI-2 import ATP-binding protein LsrA {ECO:0000250|UniProtKB:P77257};
DE EC=7.6.2.13 {ECO:0000250|UniProtKB:P77257};
GN Name=lsrA; OrderedLocusNames=SF1583, S1709;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC autoinducer 2 (AI-2) import. Responsible for energy coupling to the
CC transport system. {ECO:0000250|UniProtKB:P77257}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + (2R,4S)-2-methyl-2,3,3,4-
CC tetrahydroxytetrahydrofuran-[AI-2-binding protein]Side 1 = ADP +
CC phosphate + (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuranSide
CC 2 + [AI-2-binding protein]Side 1.; EC=7.6.2.13;
CC Evidence={ECO:0000250|UniProtKB:P77257};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC (LsrB). {ECO:0000250|UniProtKB:P77257}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P77257}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P77257}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. AI-2
CC autoinducer porter (TC 3.A.1.2.8) family. {ECO:0000305}.
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DR EMBL; AE005674; AAN43170.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP17062.1; -; Genomic_DNA.
DR RefSeq; NP_707463.1; NC_004337.2.
DR RefSeq; WP_001194865.1; NZ_WPGW01000160.1.
DR AlphaFoldDB; Q83L12; -.
DR SMR; Q83L12; -.
DR STRING; 198214.SF1583; -.
DR EnsemblBacteria; AAN43170; AAN43170; SF1583.
DR EnsemblBacteria; AAP17062; AAP17062; S1709.
DR GeneID; 1024806; -.
DR KEGG; sfl:SF1583; -.
DR KEGG; sft:NCTC1_01716; -.
DR KEGG; sfx:S1709; -.
DR PATRIC; fig|198214.7.peg.1872; -.
DR HOGENOM; CLU_000604_92_3_6; -.
DR OMA; ITHRFPE; -.
DR OrthoDB; 551294at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IEA:InterPro.
DR GO; GO:1905887; P:autoinducer AI-2 transmembrane transport; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR030281; LsrA.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43790:SF2; PTHR43790:SF2; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Repeat; Translocase; Transport.
FT CHAIN 1..511
FT /note="Autoinducer 2 import ATP-binding protein LsrA"
FT /id="PRO_0000351305"
FT DOMAIN 12..240
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 264..503
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 44..51
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 511 AA; 55731 MW; 0BC5CD116A01CC64 CRC64;
MQTSDTRALP LLCARSVYKQ YSGVNVLKGI DFTLHQGEVH ALLGGNGAGK STLMKIIAGI
TPADSGTLEI GGNNYARLTP VHAHQLGIYL VPQEPLLFPS LSIKENILFG LAKKQLSMQK
MKNLLAALGC QFDLHSLAGS LDVADRQMVE ILRGLMRDSR ILILDEPTAS LTPAETERLF
SRLQELLATG VGIVFISHKL PEIRQIADRI SVMRDGTIAL SGKTSELSTD DIIQAITPAV
REKSLSASQK LWLELPGNRP QHAVGTPVLT LENLTGEGFR NVSLTLNAGE ILGLAGLVGA
GRTELAETLY GLRTLRGGRI MLNGKEINKL STGERLLRGL VYLPEDRQSS GLNLDASLAW
NVCALTHNLR GFWAKTAKDN ATLERYRRAL NIKFNQPEQA ARTLSGSNQQ KILIAKCLEA
SPQALIVDEP TRGVDVSARN DIYQLLRSIA AQNVAVLFIS SDMEEIELMA DRVYVMHQGE
ITHSALTGRD INVETIMRVA FGDSQRQEAS C
