ID   LSRB_ECODH              Reviewed;         340 AA.
AC   B1XEA4;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Autoinducer 2-binding protein LsrB;
DE            Short=AI-2-binding protein LsrB;
DE   Flags: Precursor;
GN   Name=lsrB; OrderedLocusNames=ECDH10B_1647;
OS   Escherichia coli (strain K12 / DH10B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / DH10B;
RX   PubMed=18245285; DOI=10.1128/jb.01695-07;
RA   Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA   Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA   Posfai G., Weinstock G.M., Blattner F.R.;
RT   "The complete genome sequence of Escherichia coli DH10B: insights into the
RT   biology of a laboratory workhorse.";
RL   J. Bacteriol. 190:2597-2606(2008).
CC   -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC       autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and
CC       LsrD permeases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC       two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC       (LsrB). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 family.
CC       {ECO:0000305}.
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DR   EMBL; CP000948; ACB02726.1; -; Genomic_DNA.
DR   RefSeq; WP_000172465.1; NC_010473.1.
DR   AlphaFoldDB; B1XEA4; -.
DR   SMR; B1XEA4; -.
DR   KEGG; ecd:ECDH10B_1647; -.
DR   HOGENOM; CLU_037628_3_0_6; -.
DR   OMA; KQVNNPY; -.
DR   BioCyc; ECOL316385:ECDH10B_RS08435-MON; -.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:UniProt.
DR   CDD; cd20003; PBP1_LsrB_Quorum_Sensing; 1.
DR   InterPro; IPR030159; LsrB.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   InterPro; IPR025997; SBP_2_dom.
DR   PANTHER; PTHR46847:SF4; PTHR46847:SF4; 1.
DR   Pfam; PF13407; Peripla_BP_4; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
PE   3: Inferred from homology;
KW   Periplasm; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..340
FT                   /note="Autoinducer 2-binding protein LsrB"
FT                   /id="PRO_0000351317"
SQ   SEQUENCE   340 AA;  36685 MW;  D11AFAD3FB62A3CE CRC64;
     MTLHRFKKIA LLSALGIAAI SMNVQAAERI AFIPKLVGVG FFTSGGNGAQ QAGKELGVDV
     TYDGPTEPSV SGQVQLINNF VNQGYNAIIV SAVSPDGLCP ALKRAMQRGV RVLTWDSDTK
     PECRSYYINQ GTPAQLGGML VDMAARQVNK DKAKVAFFYS SPTVTDQNQW VKEAKAKIAK
     EHPGWEIVTT QFGYNDATKS LQTAEGILKA YSDLDAIIAP DANALPAAAQ AAENLKNDKV
     AIVGFSTPNV MRPYVERGTV KEFGLWDVVQ QGKISVYVAD ALLKKGSMKT GDKLDIKGVG
     QVEVSPNSVQ GYDYEADGNG IVLLPERVIF NKENIGKYDF