LSRB_ECOLI
ID LSRB_ECOLI Reviewed; 340 AA.
AC P76142; Q2MBA0;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Autoinducer 2-binding protein LsrB;
DE Short=AI-2-binding protein LsrB;
DE Flags: Precursor;
GN Name=lsrB; Synonyms=yneA; OrderedLocusNames=b1516, JW1509;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION IN AI-2 IMPORT, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15601708; DOI=10.1128/jb.187.1.238-248.2005;
RA Xavier K.B., Bassler B.L.;
RT "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2
RT in Escherichia coli.";
RL J. Bacteriol. 187:238-248(2005).
RN [4]
RP INDUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=15743955; DOI=10.1128/jb.187.6.2066-2076.2005;
RA Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.;
RT "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and
RT uptake of extracellular autoinducer 2 in Escherichia coli.";
RL J. Bacteriol. 187:2066-2076(2005).
CC -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and
CC LsrD permeases (Probable). {ECO:0000305|PubMed:15601708}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC (LsrB). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- INDUCTION: In the absence of AI-2, repressed by LsrR. Induced by AI-2,
CC via release of the LsrR repressor. In the absence of glucose, induced
CC by cAMP-CRP by direct binding to the upstream region of the lsr
CC promoter. {ECO:0000269|PubMed:15601708, ECO:0000269|PubMed:15743955}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 family.
CC {ECO:0000305}.
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DR EMBL; U00096; AAC74589.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76456.1; -; Genomic_DNA.
DR PIR; G64905; G64905.
DR RefSeq; NP_416033.1; NC_000913.3.
DR RefSeq; WP_000172465.1; NZ_SSZK01000001.1.
DR AlphaFoldDB; P76142; -.
DR SMR; P76142; -.
DR BioGRID; 4261349; 21.
DR ComplexPortal; CPX-4315; Autoinducer-2 ABC transporter complex.
DR IntAct; P76142; 1.
DR STRING; 511145.b1516; -.
DR TCDB; 3.A.1.2.8; the atp-binding cassette (abc) superfamily.
DR jPOST; P76142; -.
DR PaxDb; P76142; -.
DR PRIDE; P76142; -.
DR EnsemblBacteria; AAC74589; AAC74589; b1516.
DR EnsemblBacteria; BAE76456; BAE76456; BAE76456.
DR GeneID; 945418; -.
DR KEGG; ecj:JW1509; -.
DR KEGG; eco:b1516; -.
DR PATRIC; fig|1411691.4.peg.751; -.
DR EchoBASE; EB3570; -.
DR eggNOG; COG1879; Bacteria.
DR HOGENOM; CLU_037628_3_0_6; -.
DR InParanoid; P76142; -.
DR OMA; KQVNNPY; -.
DR PhylomeDB; P76142; -.
DR BioCyc; EcoCyc:YNEA-MON; -.
DR BioCyc; MetaCyc:YNEA-MON; -.
DR PRO; PR:P76142; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:UniProt.
DR GO; GO:1905887; P:autoinducer AI-2 transmembrane transport; IC:ComplexPortal.
DR GO; GO:0009372; P:quorum sensing; IC:ComplexPortal.
DR CDD; cd20003; PBP1_LsrB_Quorum_Sensing; 1.
DR InterPro; IPR030159; LsrB.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR025997; SBP_2_dom.
DR PANTHER; PTHR46847:SF4; PTHR46847:SF4; 1.
DR Pfam; PF13407; Peripla_BP_4; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 1: Evidence at protein level;
KW Periplasm; Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..340
FT /note="Autoinducer 2-binding protein LsrB"
FT /id="PRO_0000013777"
SQ SEQUENCE 340 AA; 36685 MW; D11AFAD3FB62A3CE CRC64;
MTLHRFKKIA LLSALGIAAI SMNVQAAERI AFIPKLVGVG FFTSGGNGAQ QAGKELGVDV
TYDGPTEPSV SGQVQLINNF VNQGYNAIIV SAVSPDGLCP ALKRAMQRGV RVLTWDSDTK
PECRSYYINQ GTPAQLGGML VDMAARQVNK DKAKVAFFYS SPTVTDQNQW VKEAKAKIAK
EHPGWEIVTT QFGYNDATKS LQTAEGILKA YSDLDAIIAP DANALPAAAQ AAENLKNDKV
AIVGFSTPNV MRPYVERGTV KEFGLWDVVQ QGKISVYVAD ALLKKGSMKT GDKLDIKGVG
QVEVSPNSVQ GYDYEADGNG IVLLPERVIF NKENIGKYDF
