ID   5MAT2_SALSN             Reviewed;         417 AA.
AC   Q6TXD2;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Pelargonidin 3-O-(6-caffeoylglucoside) 5-O-(6-O-malonylglucoside) 4'''-malonyltransferase;
DE            EC=2.3.1.214;
DE   AltName: Full=Anthocyanin 5-O-glucoside-4'''-O-malonyltransferase 2;
DE            Short=Ss5MaT2;
OS   Salvia splendens (Scarlet sage).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salviinae;
OC   Salvia; Salvia subgen. Calosphace; core Calosphace.
OX   NCBI_TaxID=180675;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-35 AND 388-400,
RP   FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBUNIT, BIOPHYSICOCHEMICAL
RP   PROPERTIES, ACTIVITY REGULATION, AND TISSUE SPECIFICITY.
RX   PubMed=15165190; DOI=10.1111/j.1365-313x.2004.02101.x;
RA   Suzuki H., Sawada S., Watanabe K., Nagae S., Yamaguchi M.A., Nakayama T.,
RA   Nishino T.;
RT   "Identification and characterization of a novel anthocyanin
RT   malonyltransferase from scarlet sage (Salvia splendens) flowers: an enzyme
RT   that is phylogenetically separated from other anthocyanin
RT   acyltransferases.";
RL   Plant J. 38:994-1003(2004).
CC   -!- FUNCTION: Catalyzes the transfer of the malonyl group from malonyl-CoA
CC       to the 4'''-hydroxyl group of the 5-glucosyl moiety of anthocyanins.
CC       Anthocyanins are ubiquitous colored pigments that are responsible for
CC       petal color. {ECO:0000269|PubMed:15165190}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4'''-demalonylsalvianin + malonyl-CoA = CoA + salvianin;
CC         Xref=Rhea:RHEA:35515, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384,
CC         ChEBI:CHEBI:58638, ChEBI:CHEBI:71606; EC=2.3.1.214;
CC         Evidence={ECO:0000269|PubMed:15165190};
CC   -!- ACTIVITY REGULATION: Inhibited by the following metal ions: Cd(2+),
CC       Cu(2+), Fe(2+), Hg(2+) and Zn(2+). Activity is strongly inhibited by
CC       CoA-SH and partially inhibited by acetyl-CoA, caffeic acid and
CC       bisdemalonylsalvianin. {ECO:0000269|PubMed:15165190}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=6.9 uM for malonyl-CoA (with native enzyme)
CC         {ECO:0000269|PubMed:15165190};
CC         KM=4.6 uM for malonyl-CoA (with recombinant enzyme)
CC         {ECO:0000269|PubMed:15165190};
CC         KM=51.3 uM for demalonylsalvianin (with native enzyme)
CC         {ECO:0000269|PubMed:15165190};
CC         KM=56.3 uM for demalonylsalvianin (with recombinant enzyme)
CC         {ECO:0000269|PubMed:15165190};
CC         Note=kcat is 0.55 sec(-1) (with native enzyme). kcat is 0.76 sec(-1)
CC         (with recombinant enzyme).;
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC       {ECO:0000269|PubMed:15165190}.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15165190}.
CC   -!- TISSUE SPECIFICITY: Expressed at higher level in recently opened, fully
CC       pigmented flowers. {ECO:0000269|PubMed:15165190}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AY383734; AAR26385.1; -; mRNA.
DR   AlphaFoldDB; Q6TXD2; -.
DR   SMR; Q6TXD2; -.
DR   KEGG; ag:AAR26385; -.
DR   BRENDA; 2.3.1.214; 7433.
DR   BRENDA; 2.3.1.224; 7433.
DR   SABIO-RK; Q6TXD2; -.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IDA:UniProtKB.
DR   GO; GO:0102801; F:anthocyanin 5-O-glucoside-4'''-O-malonyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0043473; P:pigmentation; IDA:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Direct protein sequencing; Transferase.
FT   CHAIN           1..417
FT                   /note="Pelargonidin 3-O-(6-caffeoylglucoside) 5-O-(6-O-
FT                   malonylglucoside) 4'''-malonyltransferase"
FT                   /id="PRO_0000422579"
FT   ACT_SITE        147
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        360
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   417 AA;  46347 MW;  617922D5E3E25D7C CRC64;
     MNINQINTKL IKPITPTPQN LKNYHISFLD QHVVKKYIAV VLYYQSAPDN GRLEDSLAET
     LVHFYPLAGR YIKTDLTVDC SDQGAEFIEA EARGDVRVTD LIGKTDTIHL CPEQYFGLDE
     GVDDPLLSIQ VTRFSCGGAT IAVSVSHRVF DVSSLETFLS AWSSASKTGG GVAPVIPSFA
     LASLLPNKDE KFGLDSNKCQ GKEQKIAVKR LLFEKRALTR LTSERTSGVR AACAVIAKAL
     IRLDRTTHGK SRDFVVFQPI NMRGRTGVPS PKNACGNMSF GSFTRRVSAK EEVGIGELVG
     LIGDGVRRGI AEYTEILCPD RDGRDVIIHV RNKNIKEVFK SETFVVSFTD WSKFGFYEVD
     FGWGRPIWSG VGPQRPRGNQ TIMMRSKEGD GIEAWVHLNE DDMDLFEQDV EIKLFLS