LSRB_SALTY
ID LSRB_SALTY Reviewed; 340 AA.
AC Q8ZKQ1;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Autoinducer 2-binding protein LsrB;
DE Short=AI-2-binding protein LsrB;
DE Flags: Precursor;
GN Name=lsrB; OrderedLocusNames=STM4077;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP FUNCTION IN AI-2 IMPORT, AND INDUCTION.
RC STRAIN=ATCC 14028 / SGSG 2980 / CDC 6516-60 / NCTC 12023;
RX PubMed=11722742; DOI=10.1046/j.1365-2958.2001.02669.x;
RA Taga M.E., Semmelhack J.L., Bassler B.L.;
RT "The LuxS-dependent autoinducer AI-2 controls the expression of an ABC
RT transporter that functions in AI-2 uptake in Salmonella typhimurium.";
RL Mol. Microbiol. 42:777-793(2001).
CC -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and
CC LsrD permeases (Probable). {ECO:0000305|PubMed:11722742}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC (LsrB). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- INDUCTION: In the absence of AI-2, repressed by LsrR. Induced by AI-2,
CC via release of the LsrR repressor. {ECO:0000269|PubMed:11722742}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 family.
CC {ECO:0000305}.
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DR EMBL; AE006468; AAL22917.1; -; Genomic_DNA.
DR RefSeq; NP_462958.1; NC_003197.2.
DR RefSeq; WP_000090737.1; NC_003197.2.
DR PDB; 1TJY; X-ray; 1.30 A; A=27-340.
DR PDB; 1TM2; X-ray; 1.90 A; A=27-340.
DR PDBsum; 1TJY; -.
DR PDBsum; 1TM2; -.
DR AlphaFoldDB; Q8ZKQ1; -.
DR SMR; Q8ZKQ1; -.
DR STRING; 99287.STM4077; -.
DR PaxDb; Q8ZKQ1; -.
DR EnsemblBacteria; AAL22917; AAL22917; STM4077.
DR GeneID; 1255604; -.
DR KEGG; stm:STM4077; -.
DR PATRIC; fig|99287.12.peg.4297; -.
DR HOGENOM; CLU_037628_3_0_6; -.
DR OMA; KQVNNPY; -.
DR PhylomeDB; Q8ZKQ1; -.
DR BioCyc; SENT99287:STM4077-MON; -.
DR EvolutionaryTrace; Q8ZKQ1; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:UniProt.
DR CDD; cd20003; PBP1_LsrB_Quorum_Sensing; 1.
DR InterPro; IPR030159; LsrB.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR025997; SBP_2_dom.
DR PANTHER; PTHR46847:SF4; PTHR46847:SF4; 1.
DR Pfam; PF13407; Peripla_BP_4; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Periplasm; Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..340
FT /note="Autoinducer 2-binding protein LsrB"
FT /id="PRO_0000351330"
FT STRAND 29..33
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 35..39
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 40..56
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 59..62
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 70..82
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 86..90
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 93..96
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 99..107
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 111..117
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 125..130
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 133..148
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 150..160
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 165..181
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 185..192
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 197..210
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 216..219
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 224..234
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 241..245
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 248..256
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 261..265
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 268..283
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 293..296
FT /evidence="ECO:0007829|PDB:1TJY"
FT TURN 297..299
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 300..305
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 307..310
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 320..323
FT /evidence="ECO:0007829|PDB:1TJY"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:1TJY"
FT TURN 332..334
FT /evidence="ECO:0007829|PDB:1TJY"
FT HELIX 335..337
FT /evidence="ECO:0007829|PDB:1TJY"
SQ SEQUENCE 340 AA; 36760 MW; 782B362C3A82B984 CRC64;
MARHSIKMIA LLTAFGLASA AMTVQAAERI AFIPKLVGVG FFTSGGNGAQ EAGKALGIDV
TYDGPTEPSV SGQVQLVNNF VNQGYDAIIV SAVSPDGLCP ALKRAMQRGV KILTWDSDTK
PECRSYYINQ GTPKQLGSML VEMAAHQVDK EKAKVAFFYS SPTVTDQNQW VKEAKAKISQ
EHPGWEIVTT QFGYNDATKS LQTAEGIIKA YPDLDAIIAP DANALPAAAQ AAENLKRNNL
AIVGFSTPNV MRPYVQRGTV KEFGLWDVVQ QGKISVYVAN ALLKNMPMNV GDSLDIPGIG
KVTVSPNSEQ GYHYEAKGNG IVLLPERVIF NKDNIDKYDF
