ID   LSRK_KLEP7              Reviewed;         532 AA.
AC   A6TEC0;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Autoinducer-2 kinase {ECO:0000255|HAMAP-Rule:MF_02053};
DE            Short=AI-2 kinase {ECO:0000255|HAMAP-Rule:MF_02053};
DE            EC=2.7.1.189 {ECO:0000255|HAMAP-Rule:MF_02053};
GN   Name=lsrK {ECO:0000255|HAMAP-Rule:MF_02053};
GN   OrderedLocusNames=KPN78578_34800; ORFNames=KPN_03509;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the phosphorylation of autoinducer-2 (AI-2) to
CC       phospho-AI-2, which subsequently inactivates the transcriptional
CC       regulator LsrR and leads to the transcription of the lsr operon.
CC       Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione
CC       (DPD), which is the precursor to all AI-2 signaling molecules, at the
CC       C5 position. {ECO:0000255|HAMAP-Rule:MF_02053}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4,5-dihydroxypentane-2,3-dione + ATP = (2S)-2-hydroxy-3,4-
CC         dioxopentyl phosphate + ADP + H(+); Xref=Rhea:RHEA:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29484, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:71677, ChEBI:CHEBI:456216; EC=2.7.1.189;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02053};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02053}.
CC   -!- SIMILARITY: Belongs to the FGGY kinase family. {ECO:0000255|HAMAP-
CC       Rule:MF_02053}.
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DR   EMBL; CP000647; ABR78904.1; -; Genomic_DNA.
DR   RefSeq; WP_015959045.1; NC_009648.1.
DR   AlphaFoldDB; A6TEC0; -.
DR   SMR; A6TEC0; -.
DR   STRING; 272620.KPN_03509; -.
DR   jPOST; A6TEC0; -.
DR   PRIDE; A6TEC0; -.
DR   DNASU; 5341441; -.
DR   EnsemblBacteria; ABR78904; ABR78904; KPN_03509.
DR   KEGG; kpn:KPN_03509; -.
DR   HOGENOM; CLU_009281_3_4_6; -.
DR   OMA; SDAMHFK; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071518; F:autoinducer-2 kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009372; P:quorum sensing; IEA:InterPro.
DR   CDD; cd07775; FGGY_AI-2K; 1.
DR   HAMAP; MF_02053; LsrK; 1.
DR   InterPro; IPR033676; AI-2_kinase.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000577; Carb_kinase_FGGY.
DR   InterPro; IPR018485; Carb_kinase_FGGY_C.
DR   InterPro; IPR018484; Carb_kinase_FGGY_N.
DR   Pfam; PF02782; FGGY_C; 1.
DR   Pfam; PF00370; FGGY_N; 1.
DR   PIRSF; PIRSF000538; GlpK; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Reference proteome; Transferase.
FT   CHAIN           1..532
FT                   /note="Autoinducer-2 kinase"
FT                   /id="PRO_0000351593"
SQ   SEQUENCE   532 AA;  57658 MW;  5CE51382DE140451 CRC64;
     MSYLLALDAG TGSIRAVIFD LNGRQLAVGQ AEWKHLSVDN VPGSMEFDLT TNWQLACQCI
     RQALDAARLS AADIQSVACC SMREGIVLYD RNGEAIWACA NVDARASREV AELKEIHDFR
     FESEVYEVSG QTLALSAMPR LLWLAHHRPD IYRKAATITM ISDWLAAKLS GELAVDPSNA
     GTTGMLDLFS RDWRPALLDM AGLRADMLSP VKETGTLLGA VTEAAAQQSG LRAGTPVVMG
     GGDVQLGCLG LGVVRAGQTA VLGGTFWQQV VNLPQVRTDP QMNIRVNPHV IPGMAQAESI
     SFFTGLTMRW FRDAFCAEEK LIAERLGVDA YSLLEEMASR VPAGSHGVMP IFSDAMHFKQ
     WYHAAPSFIN LSIDPEKCNK ATLFRALEEN AAIVSACNLA QISQFSGVTF ESLVFAGGGS
     KGALWSQILS DVTGLPVRVP VVREATALGC AIAAGTGAGL YDDMASTGER LVSWHREFTP
     NPQHRELYQE MMSKWQTVYA DQLGLVDSGL TTSMWQAPGL ERRQRVASSP SP