ID   LSRK_PHOLL              Reviewed;         530 AA.
AC   Q7N2E1;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Autoinducer-2 kinase {ECO:0000255|HAMAP-Rule:MF_02053};
DE            Short=AI-2 kinase {ECO:0000255|HAMAP-Rule:MF_02053};
DE            EC=2.7.1.189 {ECO:0000255|HAMAP-Rule:MF_02053};
GN   Name=lsrK {ECO:0000255|HAMAP-Rule:MF_02053}; OrderedLocusNames=plu3141;
OS   Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 /
OS   TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C.,
RA   Lanois A., Powell K., Siguier P., Vincent R., Wingate V., Zouine M.,
RA   Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: Catalyzes the phosphorylation of autoinducer-2 (AI-2) to
CC       phospho-AI-2, which subsequently inactivates the transcriptional
CC       regulator LsrR and leads to the transcription of the lsr operon.
CC       Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione
CC       (DPD), which is the precursor to all AI-2 signaling molecules, at the
CC       C5 position. {ECO:0000255|HAMAP-Rule:MF_02053}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4,5-dihydroxypentane-2,3-dione + ATP = (2S)-2-hydroxy-3,4-
CC         dioxopentyl phosphate + ADP + H(+); Xref=Rhea:RHEA:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29484, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:71677, ChEBI:CHEBI:456216; EC=2.7.1.189;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02053};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02053}.
CC   -!- SIMILARITY: Belongs to the FGGY kinase family. {ECO:0000255|HAMAP-
CC       Rule:MF_02053}.
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DR   EMBL; BX571869; CAE15515.1; -; Genomic_DNA.
DR   RefSeq; WP_011147352.1; NC_005126.1.
DR   AlphaFoldDB; Q7N2E1; -.
DR   SMR; Q7N2E1; -.
DR   STRING; 243265.plu3141; -.
DR   EnsemblBacteria; CAE15515; CAE15515; plu3141.
DR   GeneID; 24170041; -.
DR   KEGG; plu:plu3141; -.
DR   eggNOG; COG1070; Bacteria.
DR   HOGENOM; CLU_009281_3_4_6; -.
DR   OMA; SDAMHFK; -.
DR   OrthoDB; 1619686at2; -.
DR   BioCyc; PLUM243265:PLU_RS15635-MON; -.
DR   Proteomes; UP000002514; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071518; F:autoinducer-2 kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009372; P:quorum sensing; IEA:InterPro.
DR   CDD; cd07775; FGGY_AI-2K; 1.
DR   HAMAP; MF_02053; LsrK; 1.
DR   InterPro; IPR033676; AI-2_kinase.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000577; Carb_kinase_FGGY.
DR   InterPro; IPR018485; Carb_kinase_FGGY_C.
DR   InterPro; IPR018484; Carb_kinase_FGGY_N.
DR   Pfam; PF02782; FGGY_C; 1.
DR   Pfam; PF00370; FGGY_N; 1.
DR   PIRSF; PIRSF000538; GlpK; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Reference proteome; Transferase.
FT   CHAIN           1..530
FT                   /note="Autoinducer-2 kinase"
FT                   /id="PRO_0000351594"
SQ   SEQUENCE   530 AA;  57381 MW;  CD2776B78E117A20 CRC64;
     MNQRRSANPS GKYLMALDAG TGSVRAVIFD LEGNQIAAGQ AEWIHQPVPD VPGSMEFDLA
     TNWQLVCQCI RQALKTAQLP ASAIQGVASC SMREGIVLYN RNGEPIWACA NVDARSSQEV
     SELKALYDST FEYDVYRCSG QTLALGAMPR LLWLAHHRPD IYHQATALTM ISDWLANMLS
     GELAVDPSNA GTTGMLDLVS RDWRPDLLEM AGLRSDILSP VKETGTLLGY VTEKAAVQCG
     LNIGTPVIMG GGDVQLGCLG LGVVKPAQTA VIGGTFWQQV VNLPEPVTDP NMNTRINPHV
     IPGMVQAESI SFFTGLTMRW FRDAFCAEEK LLAERLGVDT YSLLEDMAAR VPAGAYGVIP
     IFSDVMRFKA WYHAAPSFIN LSIDPEKCNK ATLFRALEEN AAIVSACNLD LISAFSAVKP
     DSLVFAGGGS KGKLWSQILS DVTGLPVRVP MVKESTALGC AIAAGVGVGL YDAMGATGEK
     LVRWQHEYQP NPEHCEVYQK AKQDWQAIYA DQLTLVDHGL TTSLWKAPGL