ID   LSRK_YERE8              Reviewed;         529 AA.
AC   A1JJ57;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Autoinducer-2 kinase {ECO:0000255|HAMAP-Rule:MF_02053};
DE            Short=AI-2 kinase {ECO:0000255|HAMAP-Rule:MF_02053};
DE            EC=2.7.1.189 {ECO:0000255|HAMAP-Rule:MF_02053};
GN   Name=lsrK {ECO:0000255|HAMAP-Rule:MF_02053}; OrderedLocusNames=YE0530;
OS   Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 /
OS   8081).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=393305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 13174 / 8081;
RX   PubMed=17173484; DOI=10.1371/journal.pgen.0020206;
RA   Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L.,
RA   Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T.,
RA   Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S.,
RA   Sanders M., Whitehead S., Quail M.A., Dougan G., Parkhill J.,
RA   Prentice M.B.;
RT   "The complete genome sequence and comparative genome analysis of the high
RT   pathogenicity Yersinia enterocolitica strain 8081.";
RL   PLoS Genet. 2:2039-2051(2006).
CC   -!- FUNCTION: Catalyzes the phosphorylation of autoinducer-2 (AI-2) to
CC       phospho-AI-2, which subsequently inactivates the transcriptional
CC       regulator LsrR and leads to the transcription of the lsr operon.
CC       Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione
CC       (DPD), which is the precursor to all AI-2 signaling molecules, at the
CC       C5 position. {ECO:0000255|HAMAP-Rule:MF_02053}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-4,5-dihydroxypentane-2,3-dione + ATP = (2S)-2-hydroxy-3,4-
CC         dioxopentyl phosphate + ADP + H(+); Xref=Rhea:RHEA:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29484, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:71677, ChEBI:CHEBI:456216; EC=2.7.1.189;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02053};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02053}.
CC   -!- SIMILARITY: Belongs to the FGGY kinase family. {ECO:0000255|HAMAP-
CC       Rule:MF_02053}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAL10648.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AM286415; CAL10648.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_042661593.1; NC_008800.1.
DR   RefSeq; YP_001004890.1; NC_008800.1.
DR   AlphaFoldDB; A1JJ57; -.
DR   SMR; A1JJ57; -.
DR   STRING; 393305.YE0530; -.
DR   EnsemblBacteria; CAL10648; CAL10648; YE0530.
DR   KEGG; yen:YE0530; -.
DR   PATRIC; fig|393305.7.peg.621; -.
DR   eggNOG; COG1070; Bacteria.
DR   HOGENOM; CLU_009281_3_4_6; -.
DR   Proteomes; UP000000642; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071518; F:autoinducer-2 kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009372; P:quorum sensing; IEA:InterPro.
DR   CDD; cd07775; FGGY_AI-2K; 1.
DR   HAMAP; MF_02053; LsrK; 1.
DR   InterPro; IPR033676; AI-2_kinase.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000577; Carb_kinase_FGGY.
DR   InterPro; IPR018485; Carb_kinase_FGGY_C.
DR   InterPro; IPR018484; Carb_kinase_FGGY_N.
DR   Pfam; PF02782; FGGY_C; 1.
DR   Pfam; PF00370; FGGY_N; 1.
DR   PIRSF; PIRSF000538; GlpK; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Transferase.
FT   CHAIN           1..529
FT                   /note="Autoinducer-2 kinase"
FT                   /id="PRO_0000351600"
SQ   SEQUENCE   529 AA;  57032 MW;  529B81D7AB62379A CRC64;
     MSQFSTTTSG DYLMALDAGT GSVRAVIFDL NGNQIAAGQA EWLHLPVPDV PGSMEFDLTT
     NWKLTCQCIR QALHQANLPA SAIRAVAACS MREGIVLYDR SGKPIWACAN VDARASREVS
     ELKELYNNGF ELEVYQCSGQ TLALSAMPRL LWLAHYRPDI YRQAGTLTMI SDWLANMLSG
     ELAVDPSNAG TTGMLDLVTR NWQPNLLEMA GLRADILSPV KETGTLLGHV TAEAAQECGL
     LAGTPVVMGG GDVQLGCLGL GVVQPGQTAV LGGTFWQQVV NLPKPITDPN MNTRINPHVI
     PGMVQAESIS FFTGLTMRWF RDAFCAEEKL LAQRLGIDTY SLLEDMAARV PAGAYGVMPI
     FSDVMRFKSW YHAAPSFINL SLDPEKCNKA TLFRALEENA AIVSACNLAQ IAEFSGVKAS
     SVVFAGGGAK GKLWSQILAD VTGIPVRVPV VKEATALGCA IAAGVGVGLY EALDKTGEQL
     VRWEREYQPN IEHKALYQAA KTNWQAVYTD QLTLVDSGLT TSLWKAPGL