LST2_DROME
ID LST2_DROME Reviewed; 989 AA.
AC Q9VB70; Q86P84; Q8T0G9;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Lateral signaling target protein 2 homolog;
GN ORFNames=CG6051;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 438-989.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-549; SER-550 AND SER-810, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Negative regulator of epidermal growth factor receptor (EGFR)
CC signaling. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the lst-2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO25080.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF56673.3; -; Genomic_DNA.
DR EMBL; BT003320; AAO25080.1; ALT_FRAME; mRNA.
DR EMBL; AY069327; AAL39472.2; -; mRNA.
DR RefSeq; NP_001097943.1; NM_001104473.2.
DR RefSeq; NP_001247325.1; NM_001260396.1.
DR AlphaFoldDB; Q9VB70; -.
DR SMR; Q9VB70; -.
DR STRING; 7227.FBpp0293587; -.
DR PaxDb; Q9VB70; -.
DR EnsemblMetazoa; FBtr0113297; FBpp0112209; FBgn0039492.
DR EnsemblMetazoa; FBtr0305050; FBpp0293587; FBgn0039492.
DR GeneID; 43271; -.
DR KEGG; dme:Dmel_CG6051; -.
DR UCSC; CG6051-RB; d. melanogaster.
DR FlyBase; FBgn0039492; CG6051.
DR VEuPathDB; VectorBase:FBgn0039492; -.
DR eggNOG; KOG1819; Eukaryota.
DR GeneTree; ENSGT00940000171484; -.
DR HOGENOM; CLU_007360_1_0_1; -.
DR InParanoid; Q9VB70; -.
DR OMA; DAPRCMA; -.
DR OrthoDB; 451347at2759; -.
DR PhylomeDB; Q9VB70; -.
DR BioGRID-ORCS; 43271; 0 hits in 1 CRISPR screen.
DR ChiTaRS; CG6051; fly.
DR GenomeRNAi; 43271; -.
DR PRO; PR:Q9VB70; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039492; Expressed in spermathecum and 35 other tissues.
DR ExpressionAtlas; Q9VB70; baseline and differential.
DR Genevisible; Q9VB70; DM.
DR GO; GO:0031901; C:early endosome membrane; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd15731; FYVE_LST2; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR043269; FYVE_LST2.
DR InterPro; IPR000306; Znf_FYVE.
DR InterPro; IPR017455; Znf_FYVE-rel.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF01363; FYVE; 1.
DR SMART; SM00064; FYVE; 1.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS50178; ZF_FYVE; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Phosphoprotein; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..989
FT /note="Lateral signaling target protein 2 homolog"
FT /id="PRO_0000378965"
FT ZN_FING 909..969
FT /note="FYVE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT REGION 308..466
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 544..647
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 754..905
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..364
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..437
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..464
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 544..567
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..612
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 613..632
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 633..647
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 757..903
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 915
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 918
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 931
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 934
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 939
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 942
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 961
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT BINDING 964
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT MOD_RES 549
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT MOD_RES 550
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT MOD_RES 810
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT CONFLICT 356
FT /note="A -> V (in Ref. 3; AAO25080)"
FT /evidence="ECO:0000305"
FT CONFLICT 377..378
FT /note="Missing (in Ref. 3; AAO25080)"
FT /evidence="ECO:0000305"
FT CONFLICT 390
FT /note="A -> G (in Ref. 3; AAO25080)"
FT /evidence="ECO:0000305"
FT CONFLICT 476
FT /note="S -> A (in Ref. 3; AAO25080)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 989 AA; 108800 MW; 6EA7F616FB6055CC CRC64;
MDTFKRWLNK PKADDKSLLA RFFHADRSLT AVASELDSFD GRAEPDRCTR LVSRLRQNQD
KVLAITNLIM EELLGEDRDP RAFRAKFPEE VLQENLAGQL WFGAECLAAG SSIMNRETES
KEMRPLAQAV TKSLGNVRVL LRDQCLKNNV PNSKTLHLDL NDSTTEQLYE SLKIFDRLFA
EFELSYVSAM VQVKSRHEYE MQQWIGVLFS ETLQRALKIG LLDQEMVDAF DPGLMFSIPR
LAIVAGLVVY AKGPLNMDMP GDQLSEMFRP FRTILIKIRD LLRNLNNQEL YQLEKLLCTN
EDINTKVPLG SSSIEAPSPE HSSHPTTSSS QNNNNSSNNN HSSSSTNTTS TTTTTAGTTN
THRTVERLVD QRNNNHNSNS NSSTNPTVEA ATLRSPSMLS LSATSTPTAS PAPSPTPSHS
IASTSSAATS STNPPADWSD GDDEDEDDDD IDVDEEDIES SDDGTDEEQL LKDIVSADCA
SGYLIPNTNL GNLLQPQEVP LTDNFVASED DEYGTAEQQG HQGLEEDEPS TSAAMLAATR
TLQRLRLPSS DNEPLAEPTT IKASEEHMQQ PSGRHRDSHS HRHHQRHHHH HHHRHPHQHQ
HRQPHPHRTT RSGRKRCSLE AADPERIQPD REQNLASGDT SAASSLSDDV SLAMRNTTAR
LKFKSTENLL HRLFVCIAGV ADQLQTNFAS DLRQILRSVF LMNMSTAQEE IDIPEKTKES
ELFEFRASEN DVIQESAGSN QSIYSAEEVN PELDNVFSAG GGNQATGQRH SAGASMQRNN
TIDLASQPGE GSPSGATTAT SRSHVTRSRS LGDQEAASSA TSSTAQLRQL EQQQQQQQLQ
IQLQRQRNNS VGSNTPSSAS STSSSSEQNS PVSARSGSRR RLQSNNETQM PSSATSTSAT
LSPPAWIPDG KAPRCMACQT PFTAFRRRHH CRNCGGVFCG VCSNASAPLP KYGLTKAVRV
CRDCYVREVR SGMGVQGVQS VQSVQASAS
