ID   LST8_DANRE              Reviewed;         326 AA.
AC   Q803V5;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Target of rapamycin complex subunit lst8;
DE            Short=TORC subunit lst8;
DE   AltName: Full=G protein beta subunit-like;
DE            Short=Gable;
DE            Short=Protein GbetaL;
DE   AltName: Full=MTOR associated protein, LST8 homolog;
GN   Name=mlst8; Synonyms=gbl, lst8; ORFNames=zgc:55455, zgc:85668;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB; TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat LST8 family. {ECO:0000305}.
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DR   EMBL; BC044176; AAH44176.1; -; mRNA.
DR   EMBL; BC068352; AAH68352.1; -; mRNA.
DR   RefSeq; NP_001307317.1; NM_001320388.1.
DR   AlphaFoldDB; Q803V5; -.
DR   SMR; Q803V5; -.
DR   STRING; 7955.ENSDARP00000118004; -.
DR   PaxDb; Q803V5; -.
DR   Ensembl; ENSDART00000012743; ENSDARP00000019416; ENSDARG00000003167.
DR   Ensembl; ENSDART00000148326; ENSDARP00000118004; ENSDARG00000003167.
DR   Ensembl; ENSDART00000162900; ENSDARP00000137020; ENSDARG00000003167.
DR   Ensembl; ENSDART00000178845; ENSDARP00000144316; ENSDARG00000003167.
DR   GeneID; 792689; -.
DR   KEGG; dre:792689; -.
DR   CTD; 64223; -.
DR   ZFIN; ZDB-GENE-030131-6176; mlst8.
DR   eggNOG; KOG0315; Eukaryota.
DR   GeneTree; ENSGT00390000014795; -.
DR   InParanoid; Q803V5; -.
DR   OMA; VQRNYKH; -.
DR   OrthoDB; 779909at2759; -.
DR   PhylomeDB; Q803V5; -.
DR   TreeFam; TF318577; -.
DR   Reactome; R-DRE-1257604; PIP3 activates AKT signaling.
DR   Reactome; R-DRE-1632852; Macroautophagy.
DR   Reactome; R-DRE-165159; MTOR signalling.
DR   Reactome; R-DRE-166208; mTORC1-mediated signalling.
DR   Reactome; R-DRE-3371571; HSF1-dependent transactivation.
DR   Reactome; R-DRE-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR   Reactome; R-DRE-389357; CD28 dependent PI3K/Akt signaling.
DR   Reactome; R-DRE-5218920; VEGFR2 mediated vascular permeability.
DR   Reactome; R-DRE-5628897; TP53 Regulates Metabolic Genes.
DR   Reactome; R-DRE-6804757; Regulation of TP53 Degradation.
DR   Reactome; R-DRE-8943724; Regulation of PTEN gene transcription.
DR   Reactome; R-DRE-9639288; Amino acids regulate mTORC1.
DR   PRO; PR:Q803V5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 12.
DR   Bgee; ENSDARG00000003167; Expressed in testis and 26 other tissues.
DR   ExpressionAtlas; Q803V5; baseline.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0031931; C:TORC1 complex; IBA:GO_Central.
DR   GO; GO:0031932; C:TORC2 complex; IBA:GO_Central.
DR   GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0031929; P:TOR signaling; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR037588; MLST8.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   PANTHER; PTHR19842; PTHR19842; 1.
DR   Pfam; PF00400; WD40; 5.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 3.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..326
FT                   /note="Target of rapamycin complex subunit lst8"
FT                   /id="PRO_0000326502"
FT   REPEAT          1..37
FT                   /note="WD 1"
FT   REPEAT          40..80
FT                   /note="WD 2"
FT   REPEAT          83..122
FT                   /note="WD 3"
FT   REPEAT          126..165
FT                   /note="WD 4"
FT   REPEAT          168..207
FT                   /note="WD 5"
FT   REPEAT          218..257
FT                   /note="WD 6"
FT   REPEAT          268..309
FT                   /note="WD 7"
SQ   SEQUENCE   326 AA;  36212 MW;  87152D552C1401F4 CRC64;
     MNVNQGTVGS DPVILATAGY DHTVRFWQAH SGICTRTVQH QDSQVNSLEV TPDRSMIAAA
     GYQHIRMYDL NSNNPNPVIN YDGVSKNITS VGFHEDGRWM YTGGEDCMAR IWDLRSRNLQ
     CQRIFQVNAP INCVCLHPNQ AELIVGDQSG VIHIWDLKTD HNEQLIPEPD VSVNSVHIDP
     DASYMAAVNS SGNCYVWNLA GGMGDEVTQL IPKTKIPAHK RYSLRCKFSP DSTLLATCSA
     DQTCKIWRTS NFSLMTELSI KSNNPGETSR GWMWDCAFSG DSQYIVTASS DNLARLWCVE
     TGEIKREYSG HQKAVVCLAF NDSVLG