ID   LST8_XENTR              Reviewed;         326 AA.
AC   Q5I0B4; Q28HP0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Target of rapamycin complex subunit lst8;
DE            Short=TORC subunit lst8;
DE   AltName: Full=G protein beta subunit-like;
DE            Short=Gable;
DE            Short=Protein GbetaL;
DE   AltName: Full=MTOR associated protein, LST8 homolog;
GN   Name=mlst8; Synonyms=gbl, lst8; ORFNames=TTpA012a17.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Tadpole;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Tail bud;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat LST8 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAJ82929.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CR760803; CAJ82929.1; ALT_INIT; mRNA.
DR   EMBL; BC088512; AAH88512.1; -; mRNA.
DR   RefSeq; NP_001011443.1; NM_001011443.1.
DR   AlphaFoldDB; Q5I0B4; -.
DR   SMR; Q5I0B4; -.
DR   PaxDb; Q5I0B4; -.
DR   DNASU; 496930; -.
DR   GeneID; 496930; -.
DR   KEGG; xtr:496930; -.
DR   CTD; 64223; -.
DR   Xenbase; XB-GENE-981763; mlst8.
DR   eggNOG; KOG0315; Eukaryota.
DR   HOGENOM; CLU_000288_57_5_1; -.
DR   InParanoid; Q5I0B4; -.
DR   OrthoDB; 779909at2759; -.
DR   Reactome; R-XTR-1257604; PIP3 activates AKT signaling.
DR   Reactome; R-XTR-1632852; Macroautophagy.
DR   Reactome; R-XTR-165159; MTOR signalling.
DR   Reactome; R-XTR-166208; mTORC1-mediated signalling.
DR   Reactome; R-XTR-3371571; HSF1-dependent transactivation.
DR   Reactome; R-XTR-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR   Reactome; R-XTR-389357; CD28 dependent PI3K/Akt signaling.
DR   Reactome; R-XTR-5218920; VEGFR2 mediated vascular permeability.
DR   Reactome; R-XTR-5628897; TP53 Regulates Metabolic Genes.
DR   Reactome; R-XTR-6804757; Regulation of TP53 Degradation.
DR   Reactome; R-XTR-9639288; Amino acids regulate mTORC1.
DR   Proteomes; UP000008143; Chromosome 9.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0031931; C:TORC1 complex; IBA:GO_Central.
DR   GO; GO:0031932; C:TORC2 complex; IBA:GO_Central.
DR   GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0031929; P:TOR signaling; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR037588; MLST8.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   PANTHER; PTHR19842; PTHR19842; 1.
DR   Pfam; PF00400; WD40; 5.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 3.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..326
FT                   /note="Target of rapamycin complex subunit lst8"
FT                   /id="PRO_0000326504"
FT   REPEAT          1..37
FT                   /note="WD 1"
FT   REPEAT          40..80
FT                   /note="WD 2"
FT   REPEAT          83..122
FT                   /note="WD 3"
FT   REPEAT          126..165
FT                   /note="WD 4"
FT   REPEAT          168..207
FT                   /note="WD 5"
FT   REPEAT          218..257
FT                   /note="WD 6"
FT   REPEAT          268..309
FT                   /note="WD 7"
FT   CONFLICT        39
FT                   /note="Q -> L (in Ref. 1; CAJ82929)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   326 AA;  36131 MW;  08FF8EFDB6595113 CRC64;
     MNSNQGTVGS DPVILATAGY DHTVRFWQAH SGICTRTVQH QDSQVNSLEV TPDRSMIAAA
     GYQHIRMYDL NSNNPNPVIN YDGVSKNITS VGFHEDGRWM YTGGEDCMAR IWDLRSRNLQ
     CQRIFQVNAP INCVFLHPNQ AELIVGDQSG AIHIWDLKTD QNEQLIPETD VSINSVHIDP
     DASYMAAVNS SGNCFVWNLT GGLGEDLTQL IPKTKIPAHK RCALKCKFSP DSTLLATCSA
     DQTCKIWRTS NFSLMTELSI KSNNPGETSR GWMWDCAFSG DSQYIVTASS DNLARLWCVE
     TGEIKREYSG HQKAVVCLAF NDSVLG