LSY2_CAEEL
ID LSY2_CAEEL Reviewed; 365 AA.
AC Q9N5S3; Q4ZG90;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Zinc finger protein lsy-2 {ECO:0000305};
DE AltName: Full=Laterally symmetric 2 {ECO:0000312|WormBase:F49H12.1a};
GN Name=lsy-2 {ECO:0000312|WormBase:F49H12.1a};
GN ORFNames=F49H12.1 {ECO:0000312|WormBase:F49H12.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP 95-GLN--ILE-365 AND CYS-111.
RX PubMed=16291785; DOI=10.1242/dev.02163;
RA Johnston R.J. Jr., Hobert O.;
RT "A novel C. elegans zinc finger transcription factor, lsy-2, required for
RT the cell type-specific expression of the lsy-6 microRNA.";
RL Development 132:5451-5460(2005).
RN [3] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 95-GLN--ILE-365.
RX PubMed=26050091; DOI=10.1007/s13238-015-0173-1;
RA Lin L., Li Y., Yan L., Zhang G., Zhao Y., Zhang H.;
RT "LSY-2 is essential for maintaining the germ-soma distinction in C.
RT elegans.";
RL Protein Cell 6:599-609(2015).
CC -!- FUNCTION: Involved in transcriptional regulation (By similarity).
CC Required to specify left-right asymmetry of the ASE gustatory neurons,
CC probably acting upstream of microRNA lsy-6 (PubMed:16291785,
CC PubMed:26050091). Involved in maintaining the distinction between
CC somatic and germ cells, perhaps acting by repressing germ cell-specific
CC genes in somatic cells (PubMed:26050091).
CC {ECO:0000250|UniProtKB:Q9UQR1, ECO:0000269|PubMed:16291785,
CC ECO:0000269|PubMed:26050091}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:16291785}.
CC Note=Localization in nuclear speckles/punctae is dynamic, appearing to
CC move. {ECO:0000269|PubMed:16291785}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:F49H12.1a};
CC IsoId=Q9N5S3-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F49H12.1b};
CC IsoId=Q9N5S3-2; Sequence=VSP_061500;
CC -!- DEVELOPMENTAL STAGE: Expressed ubiquitously (PubMed:16291785). Earliest
CC expression is at the 50-cell stage, and persists throughout
CC embryogenesis, larval stages and adulthood (PubMed:16291785).
CC {ECO:0000269|PubMed:16291785}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes ectopic expression
CC of gcy-5 in the ASEL gustatory neuron. {ECO:0000269|PubMed:26050091}.
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DR EMBL; BX284606; CCD71575.1; -; Genomic_DNA.
DR EMBL; BX284606; CCD71576.1; -; Genomic_DNA.
DR RefSeq; NP_001024696.1; NM_001029525.3.
DR RefSeq; NP_001024697.1; NM_001029526.2.
DR AlphaFoldDB; Q9N5S3; -.
DR IntAct; Q9N5S3; 48.
DR STRING; 6239.F49H12.1a; -.
DR EPD; Q9N5S3; -.
DR PaxDb; Q9N5S3; -.
DR PeptideAtlas; Q9N5S3; -.
DR EnsemblMetazoa; F49H12.1a.1; F49H12.1a.1; WBGene00003087. [Q9N5S3-1]
DR EnsemblMetazoa; F49H12.1b.1; F49H12.1b.1; WBGene00003087. [Q9N5S3-2]
DR GeneID; 180522; -.
DR KEGG; cel:CELE_F49H12.1; -.
DR UCSC; F49H12.1a; c. elegans. [Q9N5S3-1]
DR CTD; 180522; -.
DR WormBase; F49H12.1a; CE28437; WBGene00003087; lsy-2.
DR WormBase; F49H12.1b; CE38521; WBGene00003087; lsy-2.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000171158; -.
DR HOGENOM; CLU_069900_0_0_1; -.
DR InParanoid; Q9N5S3; -.
DR OMA; AVHQCNI; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9N5S3; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003087; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q9N5S3; baseline and differential.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 5.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW Alternative splicing; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..365
FT /note="Zinc finger protein lsy-2"
FT /id="PRO_0000455602"
FT ZN_FING 78..100
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 106..128
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 134..156
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 264..287
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 296..318
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..26
FT /note="Missing (in isoform b)"
FT /id="VSP_061500"
FT MUTAGEN 95..365
FT /note="Missing: In ot64; Abolishes expression of gcy-6,
FT gcy-7, lim-6 and flp-4 in the ASEL gustatory neuron, and
FT causes ectopic expression of gcy-5 and cog-1 in ASEL.
FT Abolishes expression of microRNA gene lsy-6. Ectopic
FT expression of pgl-1 and glh-1, forming perinuclear granules
FT in various somatic cells. Protruding vulva, abnormally
FT migrating gonad arms and sterility."
FT /evidence="ECO:0000269|PubMed:16291785,
FT ECO:0000269|PubMed:26050091"
FT MUTAGEN 111
FT /note="C->Y: In ot77; Causes ectopic expression of gcy-5 in
FT ASEL gustatory neurons."
FT /evidence="ECO:0000269|PubMed:16291785"
SQ SEQUENCE 365 AA; 42288 MW; FA3E689D3EECBDD9 CRC64;
MLTRRNAKQS QRNSADQSLS EFNSSSMTHG SNQSVYHHNQ HMDDSEMMMD EQDYSQYQMG
FPEEDEMVEG MMTPRAVHQC NVCNKIFVSY KGLQQHAVIH TDQKPFRCDI CSKSFRFKSN
LFEHRSVHTG FTPHACPYCG KTCRLKGNLK KHLRTHVTTK EELEAAWRPF ASNRRPPADI
PDDAIVLRGA GGPYYTPPPR PKKKKLGLGE PEHWVDKLRR GDLLPQVELE DKIRRLEDTI
FNNMSLERWG NLFEIAKSIA FETHDCPVCK SQFMTRMDCV SHHTLEHENH REGLEFFCEK
CYRPFADEAS YNQHMSYHTR VSSLIETGEI VPQPADPEIL VPTNDEFQML FGANFGQQMM
EPQLI
