LT4R1_HUMAN
ID LT4R1_HUMAN Reviewed; 352 AA.
AC Q15722; Q13305; Q53XV5; Q92641; Q9BSU5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 193.
DE RecName: Full=Leukotriene B4 receptor 1;
DE Short=LTB4-R 1;
DE Short=LTB4-R1;
DE AltName: Full=Chemoattractant receptor-like 1;
DE AltName: Full=G-protein coupled receptor 16;
DE AltName: Full=P2Y purinoceptor 7;
DE Short=P2Y7;
GN Name=LTB4R; Synonyms=BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8702478; DOI=10.1074/jbc.271.31.18363;
RA Akbar G.K.M., Dasari V.R., Webb T., Ayyanathan K., Pillarisetti K.,
RA Sandhu A.K., Athwal R.S., Daniel J.L., Ashby B., Barnard E.A.,
RA Kunapuli S.P.;
RT "Molecular cloning of a novel P2 purinoceptor from human erythroleukemia
RT cells.";
RL J. Biol. Chem. 271:18363-18367(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8558062; DOI=10.1002/jlb.59.1.18;
RA Raport C.J., Schweickart V.L., Chantry D., Eddy R.L. Jr., Shows T.B.,
RA Godiska R., Gray P.W.;
RT "New members of the chemokine receptor gene family.";
RL J. Leukoc. Biol. 59:18-23(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9177352; DOI=10.1038/42506;
RA Yokomizo T., Izumi T., Chang K., Takuwa Y., Shimizu T.;
RT "A G-protein-coupled receptor for leukotriene B4 that mediates
RT chemotaxis.";
RL Nature 387:620-624(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8921391; DOI=10.1006/geno.1996.0541;
RA Owman C.S.O., Nilsson C., Lolait S.J.;
RT "Cloning of cDNA encoding a putative chemoattractant receptor.";
RL Genomics 37:187-194(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10934229; DOI=10.1084/jem.192.3.413;
RA Kato K., Yokomizo T., Izumi T., Shimizu T.;
RT "Cell-specific transcriptional regulation of human leukotriene B(4)
RT receptor gene.";
RL J. Exp. Med. 192:413-420(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kopatz S.A., Aronstam R.S., Sharma S.V.;
RT "Isolation of complete coding sequence for leukotriene B4 receptor
RT (LTB4R).";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PHE-346.
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PHE-346.
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP PHOSPHORYLATION, AND MUTAGENESIS OF THR-308 AND SER-310.
RX PubMed=12077128; DOI=10.1074/jbc.m202723200;
RA Gaudreau R., Le Gouill C., Venne M.-H., Stankova J., Rola-Pleszczynski M.;
RT "Threonine 308 within a putative casein kinase 2 site of the cytoplasmic
RT tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-
RT protein-coupled receptor-specific kinase 6-mediated desensitization.";
RL J. Biol. Chem. 277:31567-31576(2002).
CC -!- FUNCTION: Receptor for extracellular ATP > UTP and ADP. The activity of
CC this receptor is mediated by G proteins which activate a
CC phosphatidylinositol-calcium second messenger system. May be the
CC cardiac P2Y receptor involved in the regulation of cardiac muscle
CC contraction through modulation of L-type calcium currents. Is a
CC receptor for leukotriene B4, a potent chemoattractant involved in
CC inflammation and immune response.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed at highest levels in heart, skeletal
CC muscle and at lower levels in brain and liver. High level of expression
CC in lymphoid tissues.
CC -!- PTM: Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes
CC desensitization. {ECO:0000269|PubMed:12077128}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB16747.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U41070; AAC50628.1; -; mRNA.
DR EMBL; U33448; AAB16747.1; ALT_INIT; Genomic_DNA.
DR EMBL; D89079; BAA20424.1; -; mRNA.
DR EMBL; D89078; BAA20423.1; -; mRNA.
DR EMBL; X98356; CAA67001.1; -; mRNA.
DR EMBL; AB008193; BAB00611.1; -; Genomic_DNA.
DR EMBL; AY322535; AAP84348.1; -; Genomic_DNA.
DR EMBL; BT007267; AAP35931.1; -; mRNA.
DR EMBL; BC004545; AAH04545.1; -; mRNA.
DR CCDS; CCDS9626.1; -.
DR RefSeq; NP_001137391.1; NM_001143919.2.
DR RefSeq; NP_858043.1; NM_181657.3.
DR PDB; 7K15; X-ray; 2.88 A; A=5-310.
DR PDB; 7VKT; EM; 2.90 A; A=1-352.
DR PDBsum; 7K15; -.
DR PDBsum; 7VKT; -.
DR AlphaFoldDB; Q15722; -.
DR SMR; Q15722; -.
DR BioGRID; 107645; 10.
DR IntAct; Q15722; 2.
DR STRING; 9606.ENSP00000380008; -.
DR BindingDB; Q15722; -.
DR ChEMBL; CHEMBL3911; -.
DR DrugBank; DB06248; Amelubant.
DR DrugBank; DB12961; Leukotriene B4.
DR DrugBank; DB09285; Morniflumate.
DR DrugCentral; Q15722; -.
DR GuidetoPHARMACOLOGY; 267; -.
DR SwissLipids; SLP:000001555; -.
DR TCDB; 9.A.14.13.5; the g-protein-coupled receptor (gpcr) family.
DR GlyGen; Q15722; 2 sites.
DR iPTMnet; Q15722; -.
DR PhosphoSitePlus; Q15722; -.
DR BioMuta; LTB4R; -.
DR DMDM; 3041713; -.
DR EPD; Q15722; -.
DR jPOST; Q15722; -.
DR MassIVE; Q15722; -.
DR PaxDb; Q15722; -.
DR PeptideAtlas; Q15722; -.
DR PRIDE; Q15722; -.
DR ProteomicsDB; 60717; -.
DR Antibodypedia; 193; 533 antibodies from 33 providers.
DR DNASU; 1241; -.
DR Ensembl; ENST00000345363.8; ENSP00000307445.3; ENSG00000213903.9.
DR Ensembl; ENST00000396782.2; ENSP00000380002.2; ENSG00000213903.9.
DR Ensembl; ENST00000396789.4; ENSP00000380008.4; ENSG00000213903.9.
DR Ensembl; ENST00000646659.1; ENSP00000495785.1; ENSG00000285456.2.
DR Ensembl; ENST00000646739.1; ENSP00000494730.1; ENSG00000285456.2.
DR Ensembl; ENST00000647085.2; ENSP00000495466.1; ENSG00000285456.2.
DR GeneID; 1241; -.
DR KEGG; hsa:1241; -.
DR MANE-Select; ENST00000345363.8; ENSP00000307445.3; NM_001143919.3; NP_001137391.1.
DR UCSC; uc001wos.4; human.
DR CTD; 1241; -.
DR DisGeNET; 1241; -.
DR GeneCards; LTB4R; -.
DR HGNC; HGNC:6713; LTB4R.
DR HPA; ENSG00000213903; Tissue enhanced (esophagus, skin).
DR MIM; 601531; gene.
DR neXtProt; NX_Q15722; -.
DR OpenTargets; ENSG00000213903; -.
DR PharmGKB; PA30476; -.
DR VEuPathDB; HostDB:ENSG00000213903; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00950000182966; -.
DR HOGENOM; CLU_009579_8_0_1; -.
DR InParanoid; Q15722; -.
DR OMA; CRLRSAM; -.
DR OrthoDB; 978296at2759; -.
DR PhylomeDB; Q15722; -.
DR TreeFam; TF330976; -.
DR PathwayCommons; Q15722; -.
DR Reactome; R-HSA-391906; Leukotriene receptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR SignaLink; Q15722; -.
DR SIGNOR; Q15722; -.
DR BioGRID-ORCS; 1241; 27 hits in 1080 CRISPR screens.
DR ChiTaRS; LTB4R; human.
DR GeneWiki; Leukotriene_B4_receptor; -.
DR GenomeRNAi; 1241; -.
DR Pharos; Q15722; Tchem.
DR PRO; PR:Q15722; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q15722; protein.
DR Bgee; ENSG00000213903; Expressed in lower esophagus mucosa and 97 other tissues.
DR ExpressionAtlas; Q15722; baseline and differential.
DR Genevisible; Q15722; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0001632; F:leukotriene B4 receptor activity; IBA:GO_Central.
DR GO; GO:0004974; F:leukotriene receptor activity; TAS:ProtInc.
DR GO; GO:0000166; F:nucleotide binding; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0006955; P:immune response; TAS:ProtInc.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0006936; P:muscle contraction; TAS:ProtInc.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR003981; Leukotriene_B4_rcpt.
DR InterPro; IPR003983; Leukotriene_B4_typ-1_rcpt.
DR PANTHER; PTHR24230:SF51; PTHR24230:SF51; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01477; LTB1RECEPTOR.
DR PRINTS; PR01476; LTBRECEPTOR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..352
FT /note="Leukotriene B4 receptor 1"
FT /id="PRO_0000069708"
FT TOPO_DOM 1..19
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..42
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..91
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..113
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..268
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 310..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..352
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 164
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 346
FT /note="L -> F (in dbSNP:rs17849864)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.7"
FT /id="VAR_060679"
FT MUTAGEN 308
FT /note="T->P,A: No effect on affinity for leukotriene B4,
FT induces resistance to desensitization by GRK6, but minor
FT effect on phosphorylation by GRK6."
FT /evidence="ECO:0000269|PubMed:12077128"
FT MUTAGEN 310
FT /note="S->A: No effect on affinity for leukotriene B4 or on
FT desensitization by GRK6."
FT /evidence="ECO:0000269|PubMed:12077128"
FT CONFLICT 246
FT /note="G -> R (in Ref. 4; CAA67001)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="A -> V (in Ref. 1; AAC50628)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="L -> V (in Ref. 1; AAC50628)"
FT /evidence="ECO:0000305"
FT HELIX 14..44
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 52..68
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 71..80
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 86..120
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 122..128
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 131..148
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 152..155
FT /evidence="ECO:0007829|PDB:7K15"
FT STRAND 156..160
FT /evidence="ECO:0007829|PDB:7K15"
FT STRAND 166..170
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 175..189
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 191..208
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 221..249
FT /evidence="ECO:0007829|PDB:7K15"
FT STRAND 257..259
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 260..277
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 280..292
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 294..296
FT /evidence="ECO:0007829|PDB:7K15"
FT HELIX 298..305
FT /evidence="ECO:0007829|PDB:7K15"
FT STRAND 307..309
FT /evidence="ECO:0007829|PDB:7K15"
SQ SEQUENCE 352 AA; 37557 MW; 5A7BFC0A659AC81C CRC64;
MNTTSSAAPP SLGVEFISLL AIILLSVALA VGLPGNSFVV WSILKRMQKR SVTALMVLNL
ALADLAVLLT APFFLHFLAQ GTWSFGLAGC RLCHYVCGVS MYASVLLITA MSLDRSLAVA
RPFVSQKLRT KAMARRVLAG IWVLSFLLAT PVLAYRTVVP WKTNMSLCFP RYPSEGHRAF
HLIFEAVTGF LLPFLAVVAS YSDIGRRLQA RRFRRSRRTG RLVVLIILTF AAFWLPYHVV
NLAEAGRALA GQAAGLGLVG KRLSLARNVL IALAFLSSSV NPVLYACAGG GLLRSAGVGF
VAKLLEGTGS EASSTRRGGS LGQTARSGPA ALEPGPSESL TASSPLKLNE LN
