ID   LTAE_PSEAE              Reviewed;         346 AA.
AC   Q9HTF1;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Low specificity L-threonine aldolase;
DE            Short=Low specificity L-TA;
DE            EC=4.1.2.48;
GN   Name=ltaE; OrderedLocusNames=PA5413;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the cleavage of L-allo-threonine and L-threonine to
CC       glycine and acetaldehyde. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-threonine = acetaldehyde + glycine; Xref=Rhea:RHEA:19625,
CC         ChEBI:CHEBI:15343, ChEBI:CHEBI:57305, ChEBI:CHEBI:57926; EC=4.1.2.48;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-allo-threonine = acetaldehyde + glycine;
CC         Xref=Rhea:RHEA:26209, ChEBI:CHEBI:15343, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:58585; EC=4.1.2.48;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the threonine aldolase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG08798.1; -; Genomic_DNA.
DR   PIR; A82971; A82971.
DR   RefSeq; NP_254100.1; NC_002516.2.
DR   RefSeq; WP_003105702.1; NZ_QZGE01000031.1.
DR   AlphaFoldDB; Q9HTF1; -.
DR   SMR; Q9HTF1; -.
DR   STRING; 287.DR97_2790; -.
DR   PaxDb; Q9HTF1; -.
DR   PRIDE; Q9HTF1; -.
DR   EnsemblBacteria; AAG08798; AAG08798; PA5413.
DR   GeneID; 880064; -.
DR   KEGG; pae:PA5413; -.
DR   PATRIC; fig|208964.12.peg.5673; -.
DR   PseudoCAP; PA5413; -.
DR   HOGENOM; CLU_049619_0_0_6; -.
DR   InParanoid; Q9HTF1; -.
DR   OMA; ETDCEVF; -.
DR   PhylomeDB; Q9HTF1; -.
DR   BioCyc; PAER208964:G1FZ6-5540-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0008732; F:L-allo-threonine aldolase activity; IEA:RHEA.
DR   GO; GO:0006567; P:threonine catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR   InterPro; IPR026273; Low_specificity_L-TA_bact.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF01212; Beta_elim_lyase; 1.
DR   PIRSF; PIRSF038940; Low_specificity_LTA; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
PE   3: Inferred from homology;
KW   Lyase; Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..346
FT                   /note="Low specificity L-threonine aldolase"
FT                   /id="PRO_0000121577"
FT   MOD_RES         207
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   346 AA;  38213 MW;  52129E3422DAC845 CRC64;
     MTDHTQQFAS DNYSGICPEA WAAMAEANRG HERAYGDDQW TARASDYFRQ LFETDCEVFF
     AFNGTAANSL ALAALCQSYH SVICSETAHV ETDECGAPEF FSNGSKLLLA QTEVGKLTPA
     SIRDIALKRQ DIHYPKPRVV TLTQATEVGT VYRPDELKAI SATCKELGLH LHMDGARFSN
     ACAFLGCSPA ELSWKAGVDV LCFGGTKNGM AVGEAILFFN RDLAEDFDYR CKQAGQLASK
     MRFLAAPWVG VLQDDAWLRY ADHANRCARL LAELVADVPG VSLMFPVEAN GVFLQLSEPA
     IEALRARGWR FYTFIGEGGA RFMCSWDTDI ERVRELARDI RLVMGA