ID   LTE1_KLULA              Reviewed;        1366 AA.
AC   Q6CY10;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Guanine nucleotide exchange factor LTE1;
GN   Name=LTE1; OrderedLocusNames=KLLA0A04059g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: GDP-GTP exchange factor component of the mitotic exit network
CC       (MEN). Fine-tunes the timing of the mitotic exit and couples this event
CC       with cytokinesis. May also be involved in proprotein-processing in the
CC       secretory pathway (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Bud {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LTE1 family. {ECO:0000305}.
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DR   EMBL; CR382121; CAH02767.1; -; Genomic_DNA.
DR   RefSeq; XP_451179.1; XM_451179.1.
DR   AlphaFoldDB; Q6CY10; -.
DR   SMR; Q6CY10; -.
DR   STRING; 28985.XP_451179.1; -.
DR   PRIDE; Q6CY10; -.
DR   EnsemblFungi; CAH02767; CAH02767; KLLA0_A04059g.
DR   GeneID; 2896846; -.
DR   KEGG; kla:KLLA0_A04059g; -.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_004883_0_0_1; -.
DR   InParanoid; Q6CY10; -.
DR   OMA; PFILMYD; -.
DR   Proteomes; UP000000598; Chromosome A.
DR   GO; GO:0005933; C:cellular bud; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS00720; RASGEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Cytoplasm; Guanine-nucleotide releasing factor;
KW   Mitosis; Reference proteome.
FT   CHAIN           1..1366
FT                   /note="Guanine nucleotide exchange factor LTE1"
FT                   /id="PRO_0000285406"
FT   DOMAIN          37..170
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          1121..1361
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          290..314
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          661..690
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          955..979
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        299..314
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        675..690
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1366 AA;  155254 MW;  A631909AB6B6A65A CRC64;
     MSEVVEKADE NVVPLFSRPE LYPVPSESVL TVDLRDGTVR ILKCTLPALL VQLSSPLDKV
     DYSMLTDFFL VYRNFMSSQK LLDLIQERFQ WAIDLRQSDP SSQEKITSEV ILVRMFVLVR
     HWLSNYFAQD FVVDTSLRRQ FLQFINGYSP VDRFLDNIII SLKKLWVQNV QIMWEDLENL
     IVENNVVSRD DWLKWEIEDV PSGSSSETGE GSTKGKRLSF IALQNINNPV LRNESLLSLL
     HTREKIPLPQ QSEDESKKQS TRIKQRTGSM LLFPENASNG LSINEKLTAS GVSPEATPKQ
     EREITDPKNS KDNKHILPQL SRVTNVSYMM KDLEYPSTPS VDIFVPPTPA KNIEFILQTS
     YMETDLPEQS NGSDSTNTVN GNNTNHKGIL GLLSKWKLNH QRKPTVNPVQ NPPRVENLIK
     YVFSISSLDN HANPLPDSLS SMFDILSART IDEVEYLVSV ESDLLAKLEA KKLTTEISKL
     ETNEDESQDY SVIDNLNLYK TVSSIANSVI SLSKTLNVRT NKSTTHLLSP STSALERKNI
     RNSAPMLYSY NNSRYSITNA LMGLPNTNDN SSPKRLVFHD PTRNSPTKKA ILANNLNNIG
     EYNGERDSIT SIVTYDSAFS DISSSGNILS QHKGSSNIFM ESAPTLKRKL NVNDLRRFNF
     EKSDSTDDRS CSPQNREATE TSATSMESDA NDNAIQDEYE NENEDIASLI TAYEESTSEI
     DTQCQNNAQI RRPTSGRISI TRNYSVASPN SLRSILPKSP LILGNEVFIE RDKALAANQD
     IISELEETTS LLLNDNDRKF TVSRGSVCND SDSQSISTNL LFSSAQASPQ KLVIKEVDVI
     QEKSEHPDVP TPVLCKQPIS KLSETPSIKS IGSRDSEESF DLTSIASKPN RAQSTTLREK
     YHLEKQATND IFEEDVENLN PENNKYLFSP DTDSIDFASP EKNLDDLKQQ FIDQSTDEQT
     SLDEEEIATQ DNENKDHGID KKKLEDIMNG IDDTADTSMD PVNLALMKLE GTYDKGEKEI
     DDKSPSINSE LAREVENFQI VQTAALPESA RKRQSMFIQR RRNTMIDFSV RDSLIDKDSS
     CTRLENTDEQ IRNLLNQYTL TDSRLKIDNL EQHIPFILMY DSKSVANQLT LIEKEILSEV
     DWKDLLDLTM SEQLPQFTSW LQLLVQNENL SGIDLAIARF NLTVDWIISE IVMTQDIRLR
     RNTIQRFIHI AEHCKELQNY NTLMEIILAL NSIVVQKFTE TWRLVEPGDL LTWETLKAIP
     SLEKNYSNIR QLIDEVEPLS GCIPFIVVYL SDLSLNIEKR TWIVHDEVLN YNKFQTNVQI
     VKNFVQKMQW SKFYNIDIDH ELLSKCVYIT SLSHDEINSI SHKSPI