LTG16_ARATH
ID LTG16_ARATH Reviewed; 200 AA.
AC Q94AX3;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Non-specific lipid transfer protein GPI-anchored 16 {ECO:0000303|PubMed:23893219};
DE Short=AtLTPG-16 {ECO:0000303|PubMed:23893219};
DE Short=Protein LTP-GPI-ANCHORED 16 {ECO:0000303|PubMed:23893219};
DE AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 4 {ECO:0000303|PubMed:15634699};
DE AltName: Full=Xylogen like protein 6 {ECO:0000303|Ref.1};
DE Short=AtXYP6 {ECO:0000303|Ref.1};
DE Flags: Precursor;
GN Name=LTPG16 {ECO:0000303|PubMed:23893219};
GN Synonyms=EDA4 {ECO:0000303|PubMed:15634699}, XYP6 {ECO:0000303|Ref.1};
GN OrderedLocusNames=At2g48140 {ECO:0000312|Araport:AT2G48140};
GN ORFNames=F11L15.4 {ECO:0000312|EMBL:AEC10944.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kobayashi Y., Sawa S., Iwamoto K., Motose H., Fukuda H.;
RT "Xylogen family genes, involved in cell-cell communication in
RT Arabidopsis.";
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [7]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=23893219; DOI=10.1007/s11103-013-0113-5;
RA Edstam M.M., Blomqvist K., Ekloef A., Wennergren U., Edqvist J.;
RT "Coexpression patterns indicate that GPI-anchored non-specific lipid
RT transfer proteins are involved in accumulation of cuticular wax, suberin
RT and sporopollenin.";
RL Plant Mol. Biol. 83:625-649(2013).
CC -!- FUNCTION: Essential protein involved in female gametophyte development
CC (PubMed:15634699). Probable lipid transfer protein (By similarity).
CC {ECO:0000250|UniProtKB:Q9C7F7, ECO:0000269|PubMed:15634699}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Lipid-anchor, GPI-
CC anchor {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, preferentially in
CC hypocotyls and roots (PubMed:23893219). Also observed in siliques
CC (PubMed:23893219). {ECO:0000269|PubMed:23893219}.
CC -!- DISRUPTION PHENOTYPE: Female gametophyte disrupted development arrested
CC at the four-nuclear stage. {ECO:0000269|PubMed:15634699}.
CC -!- SIMILARITY: Belongs to the plant LTP family. {ECO:0000305}.
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DR EMBL; AB246325; BAE73262.1; -; mRNA.
DR EMBL; LR782543; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002685; AEC10944.1; -; Genomic_DNA.
DR EMBL; AY045643; AAK74001.1; -; mRNA.
DR EMBL; AY059652; AAL31145.1; -; mRNA.
DR EMBL; AY088054; AAM65600.1; -; mRNA.
DR RefSeq; NP_566127.1; NM_130381.3.
DR AlphaFoldDB; Q94AX3; -.
DR STRING; 3702.AT2G48140.1; -.
DR PaxDb; Q94AX3; -.
DR ProteomicsDB; 187459; -.
DR EnsemblPlants; AT2G48140.1; AT2G48140.1; AT2G48140.
DR GeneID; 819426; -.
DR Gramene; AT2G48140.1; AT2G48140.1; AT2G48140.
DR KEGG; ath:AT2G48140; -.
DR Araport; AT2G48140; -.
DR TAIR; locus:2039341; AT2G48140.
DR eggNOG; ENOG502RZXE; Eukaryota.
DR HOGENOM; CLU_085549_2_0_1; -.
DR InParanoid; Q94AX3; -.
DR OMA; KICHISV; -.
DR OrthoDB; 1573578at2759; -.
DR PhylomeDB; Q94AX3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q94AX3; baseline and differential.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009561; P:megagametogenesis; IMP:TAIR.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR043325; LTSS.
DR PANTHER; PTHR33044; PTHR33044; 1.
DR Pfam; PF14368; LTP_2; 1.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW Membrane; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..177
FT /note="Non-specific lipid transfer protein GPI-anchored 16"
FT /id="PRO_5014312520"
FT PROPEP 178..200
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000451647"
FT REGION 134..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 177
FT /note="GPI-anchor amidated threonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 27..72
FT /evidence="ECO:0000250|UniProtKB:A0A0B4JDK1"
FT DISULFID 38..56
FT /evidence="ECO:0000250|UniProtKB:A0A0B4JDK1"
FT DISULFID 57..98
FT /evidence="ECO:0000250|UniProtKB:A0A0B4JDK1"
FT DISULFID 70..107
FT /evidence="ECO:0000250|UniProtKB:A0A0B4JDK1"
SQ SEQUENCE 200 AA; 20145 MW; E467B204F59EA937 CRC64;
MEGLTLIVVM MSSFMLGGQG QQISTPCTSS MISTFTPCLN FITGSSGGSV TPTAGCCDSL
KTLTNTGMGC ACLILTANVP LPTGFINRTL ALALPRACKM GGVPIQCQAA GTPLPAPGQV
PFLIAPPPQV SAFSPGASKA AGTTPTQAPA PDTPADGPTG PTTKSGIRPV DQPMQPTGLA
QSSTSPFLPL LFISLILLNL
