ID   LTOR2_BOVIN             Reviewed;         125 AA.
AC   Q3T132;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Ragulator complex protein LAMTOR2;
DE   AltName: Full=Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2;
GN   Name=LAMTOR2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: As part of the Ragulator complex it is involved in amino acid
CC       sensing and activation of mTORC1, a signaling complex promoting cell
CC       growth in response to growth factors, energy levels, and amino acids.
CC       Activated by amino acids through a mechanism involving the lysosomal V-
CC       ATPase, the Ragulator functions as a guanine nucleotide exchange factor
CC       activating the small GTPases Rag. Activated Ragulator and Rag GTPases
CC       function as a scaffold recruiting mTORC1 to lysosomes where it is in
CC       turn activated. Adapter protein that enhances the efficiency of the MAP
CC       kinase cascade facilitating the activation of MAPK2 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Part of the Ragulator complex composed of LAMTOR1, LAMTOR2,
CC       LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer
CC       that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer.
CC       Interacts with LAMTOR1 and LAMTOR3; the interaction is direct. The
CC       Ragulator complex interacts with both the mTORC1 complex and
CC       heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and
CC       RRAGD; regulated by amino acid availability. The Ragulator complex
CC       interacts with SLC38A9; the probable amino acid sensor (By similarity).
CC       Interacts with MAPK1 and MAP2K1 (By similarity). Component of the
CC       lysosomal folliculin complex (LFC), composed of FLCN, FNIP1 (or FNIP2),
CC       RRAGA/RagA or RRAGB/RagB GDP-bound, RRAGC/RagC or RRAGD/RagD GTP-bound,
CC       and Ragulator (By similarity). {ECO:0000250|UniProtKB:Q9JHS3,
CC       ECO:0000250|UniProtKB:Q9Y2Q5}.
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Lysosome membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GAMAD family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC102146; AAI02147.1; -; mRNA.
DR   RefSeq; NP_001029963.1; NM_001034791.1.
DR   AlphaFoldDB; Q3T132; -.
DR   BMRB; Q3T132; -.
DR   SMR; Q3T132; -.
DR   STRING; 9913.ENSBTAP00000045185; -.
DR   PaxDb; Q3T132; -.
DR   PRIDE; Q3T132; -.
DR   Ensembl; ENSBTAT00000048084; ENSBTAP00000045185; ENSBTAG00000011754.
DR   GeneID; 616375; -.
DR   KEGG; bta:616375; -.
DR   CTD; 28956; -.
DR   VEuPathDB; HostDB:ENSBTAG00000011754; -.
DR   VGNC; VGNC:30780; LAMTOR2.
DR   eggNOG; KOG4107; Eukaryota.
DR   GeneTree; ENSGT00390000006100; -.
DR   HOGENOM; CLU_141118_0_0_1; -.
DR   InParanoid; Q3T132; -.
DR   OMA; WAAYEKN; -.
DR   OrthoDB; 1515483at2759; -.
DR   TreeFam; TF313929; -.
DR   Reactome; R-BTA-1632852; Macroautophagy.
DR   Reactome; R-BTA-165159; MTOR signalling.
DR   Reactome; R-BTA-166208; mTORC1-mediated signalling.
DR   Reactome; R-BTA-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR   Reactome; R-BTA-5628897; TP53 Regulates Metabolic Genes.
DR   Reactome; R-BTA-5674135; MAP2K and MAPK activation.
DR   Reactome; R-BTA-6798695; Neutrophil degranulation.
DR   Reactome; R-BTA-9639288; Amino acids regulate mTORC1.
DR   Proteomes; UP000009136; Chromosome 3.
DR   Bgee; ENSBTAG00000011754; Expressed in oocyte and 104 other tissues.
DR   GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0071986; C:Ragulator complex; ISS:UniProtKB.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:Ensembl.
DR   GO; GO:0060090; F:molecular adaptor activity; IEA:Ensembl.
DR   GO; GO:0071230; P:cellular response to amino acid stimulus; ISS:UniProtKB.
DR   GO; GO:0010761; P:fibroblast migration; IEA:Ensembl.
DR   GO; GO:0043410; P:positive regulation of MAPK cascade; IEA:Ensembl.
DR   GO; GO:0032008; P:positive regulation of TOR signaling; ISS:UniProtKB.
DR   GO; GO:0008104; P:protein localization; ISS:UniProtKB.
DR   GO; GO:1902414; P:protein localization to cell junction; IEA:Ensembl.
DR   GO; GO:0001558; P:regulation of cell growth; ISS:UniProtKB.
DR   GO; GO:0150116; P:regulation of cell-substrate junction organization; IEA:Ensembl.
DR   InterPro; IPR037587; LAMTOR2-like.
DR   InterPro; IPR004942; Roadblock/LAMTOR2_dom.
DR   PANTHER; PTHR13323; PTHR13323; 1.
DR   Pfam; PF03259; Robl_LC7; 1.
DR   SMART; SM00960; Robl_LC7; 1.
PE   2: Evidence at transcript level;
KW   Endosome; Lysosome; Membrane; Reference proteome.
FT   CHAIN           1..125
FT                   /note="Ragulator complex protein LAMTOR2"
FT                   /id="PRO_0000240660"
FT   REGION          57..70
FT                   /note="Required for location at endosomes"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   125 AA;  13508 MW;  69599211C2CBC03D CRC64;
     MLRPKALTQV LSQANTGGVQ STLLLNNEGS LLAYSGYGDT DARVTAAIAS NIWAAYDRNG
     NQAFNEDNLK FILMDCMEGR VAITRVANLL LCMYAKETVG FGMLKAKAQA LVQYLEEPLT
     QVAAS