LTOR2_DROME
ID LTOR2_DROME Reviewed; 125 AA.
AC Q9V8I2; A8E6I2; B7YZK0; Q6NP73;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Ragulator complex protein LAMTOR2 homolog;
DE AltName: Full=Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2;
GN ORFNames=CG5189;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Booth B.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R.A., Gonzalez M., Guarin H., Kapadia B., Kronmiller B., Li P.W.,
RA Liao G., Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V.,
RA Park S., Patel S., Phouanenavong S., Sandler J., Wan K.H., Yu C.,
RA Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=20381137; DOI=10.1016/j.cell.2010.02.024;
RA Sancak Y., Bar-Peled L., Zoncu R., Markhard A.L., Nada S., Sabatini D.M.;
RT "Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is
RT necessary for its activation by amino acids.";
RL Cell 141:290-303(2010).
CC -!- FUNCTION: Regulator of the TOR pathway, a signaling cascade that
CC promotes cell growth in response to growth factors, energy levels, and
CC amino acids. As part of the Ragulator complex, may activate the TOR
CC signaling cascade in response to amino acids.
CC {ECO:0000269|PubMed:20381137}.
CC -!- SUBUNIT: Part of the Ragulator complex composed of CG5110, CG5189,
CC CG14184, CG14812, and CG14977. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9V8I2; Q9VJD2: CG5110; NbExp=6; IntAct=EBI-98229, EBI-111967;
CC -!- SIMILARITY: Belongs to the GAMAD family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57685.1; -; Genomic_DNA.
DR EMBL; AE013599; ACL83153.1; -; Genomic_DNA.
DR EMBL; BT011055; AAR31126.1; -; mRNA.
DR EMBL; BT030774; ABV82156.1; -; mRNA.
DR EMBL; BT058093; ACM86249.1; -; mRNA.
DR RefSeq; NP_001137699.1; NM_001144227.2.
DR RefSeq; NP_611334.1; NM_137490.3.
DR AlphaFoldDB; Q9V8I2; -.
DR SMR; Q9V8I2; -.
DR BioGRID; 62798; 2.
DR DIP; DIP-21855N; -.
DR IntAct; Q9V8I2; 2.
DR STRING; 7227.FBpp0289083; -.
DR PaxDb; Q9V8I2; -.
DR PRIDE; Q9V8I2; -.
DR DNASU; 37122; -.
DR EnsemblMetazoa; FBtr0086694; FBpp0085873; FBgn0034350.
DR EnsemblMetazoa; FBtr0299805; FBpp0289083; FBgn0034350.
DR GeneID; 37122; -.
DR KEGG; dme:Dmel_CG5189; -.
DR UCSC; CG5189-RA; d. melanogaster.
DR FlyBase; FBgn0034350; CG5189.
DR VEuPathDB; VectorBase:FBgn0034350; -.
DR eggNOG; KOG4107; Eukaryota.
DR GeneTree; ENSGT00390000006100; -.
DR HOGENOM; CLU_141118_0_0_1; -.
DR InParanoid; Q9V8I2; -.
DR OMA; WAAYEKN; -.
DR PhylomeDB; Q9V8I2; -.
DR Reactome; R-DME-1632852; Macroautophagy.
DR Reactome; R-DME-165159; MTOR signalling.
DR Reactome; R-DME-166208; mTORC1-mediated signalling.
DR Reactome; R-DME-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR Reactome; R-DME-5628897; TP53 Regulates Metabolic Genes.
DR Reactome; R-DME-5674135; MAP2K and MAPK activation.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR Reactome; R-DME-8943724; Regulation of PTEN gene transcription.
DR Reactome; R-DME-9639288; Amino acids regulate mTORC1.
DR BioGRID-ORCS; 37122; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 37122; -.
DR PRO; PR:Q9V8I2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034350; Expressed in Malpighian tubule and 11 other tissues.
DR Genevisible; Q9V8I2; DM.
DR GO; GO:0071986; C:Ragulator complex; IMP:FlyBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0060090; F:molecular adaptor activity; IEA:InterPro.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; IMP:FlyBase.
DR GO; GO:0032008; P:positive regulation of TOR signaling; IMP:UniProtKB.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IMP:FlyBase.
DR GO; GO:0008104; P:protein localization; ISS:UniProtKB.
DR GO; GO:0001558; P:regulation of cell growth; ISS:UniProtKB.
DR InterPro; IPR037587; LAMTOR2-like.
DR InterPro; IPR004942; Roadblock/LAMTOR2_dom.
DR PANTHER; PTHR13323; PTHR13323; 1.
DR Pfam; PF03259; Robl_LC7; 1.
DR SMART; SM00960; Robl_LC7; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..125
FT /note="Ragulator complex protein LAMTOR2 homolog"
FT /id="PRO_0000220963"
FT CONFLICT 80
FT /note="H -> L (in Ref. 3; AAR31126)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 125 AA; 13439 MW; 2C1E2A6199225AD1 CRC64;
MLKPKALTQV LSQANTGGVE NTLLLSQEGA LLAYSGYGDK DARITAAIAS NIWAAYEKHG
RNAFREGRLT FVLIDCENGH VAITQVASVL LCLYAKQTVG LGLLKQKAMS LASYLERPLK
QISAS
