LTOR4_DROME
ID LTOR4_DROME Reviewed; 120 AA.
AC Q9VZL6; B3DML9; Q8SXW8;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Ragulator complex protein LAMTOR4 homolog;
DE AltName: Full=Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4;
GN ORFNames=CG14977;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION IN TOR PATHWAY.
RX PubMed=22980980; DOI=10.1016/j.cell.2012.07.032;
RA Bar-Peled L., Schweitzer L.D., Zoncu R., Sabatini D.M.;
RT "Ragulator is a GEF for the Rag GTPases that signal amino acid levels to
RT mTORC1.";
RL Cell 150:1196-1208(2012).
CC -!- FUNCTION: Regulator of the TOR pathway, a signaling cascade that
CC promotes cell growth in response to growth factors, energy levels, and
CC amino acids. As part of the Ragulator complex, may activate the TOR
CC signaling cascade in response to amino acids.
CC {ECO:0000269|PubMed:22980980}.
CC -!- SUBUNIT: Part of the Ragulator complex composed of CG5110, CG5189,
CC CG14184, CG14812, and CG14977. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LAMTOR4 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF47804.2; -; Genomic_DNA.
DR EMBL; AY075542; AAL68349.1; -; mRNA.
DR EMBL; BT032657; ACD81671.1; -; mRNA.
DR RefSeq; NP_647834.3; NM_139577.3.
DR AlphaFoldDB; Q9VZL6; -.
DR SMR; Q9VZL6; -.
DR BioGRID; 63942; 3.
DR IntAct; Q9VZL6; 2.
DR STRING; 7227.FBpp0073016; -.
DR PaxDb; Q9VZL6; -.
DR PRIDE; Q9VZL6; -.
DR DNASU; 38454; -.
DR EnsemblMetazoa; FBtr0073158; FBpp0073016; FBgn0035469.
DR GeneID; 38454; -.
DR KEGG; dme:Dmel_CG14977; -.
DR UCSC; CG14977-RA; d. melanogaster.
DR FlyBase; FBgn0035469; CG14977.
DR VEuPathDB; VectorBase:FBgn0035469; -.
DR eggNOG; ENOG502S3B2; Eukaryota.
DR GeneTree; ENSGT00390000016053; -.
DR HOGENOM; CLU_137556_0_0_1; -.
DR InParanoid; Q9VZL6; -.
DR OMA; DESFMPN; -.
DR OrthoDB; 1589714at2759; -.
DR PhylomeDB; Q9VZL6; -.
DR Reactome; R-DME-1632852; Macroautophagy.
DR Reactome; R-DME-165159; MTOR signalling.
DR Reactome; R-DME-166208; mTORC1-mediated signalling.
DR Reactome; R-DME-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR Reactome; R-DME-5628897; TP53 Regulates Metabolic Genes.
DR Reactome; R-DME-8943724; Regulation of PTEN gene transcription.
DR Reactome; R-DME-9639288; Amino acids regulate mTORC1.
DR BioGRID-ORCS; 38454; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38454; -.
DR PRO; PR:Q9VZL6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035469; Expressed in saliva-secreting gland and 28 other tissues.
DR ExpressionAtlas; Q9VZL6; baseline and differential.
DR Genevisible; Q9VZL6; DM.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0071986; C:Ragulator complex; ISS:UniProtKB.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; IMP:UniProtKB.
DR GO; GO:0032008; P:positive regulation of TOR signaling; IMP:UniProtKB.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IMP:FlyBase.
DR GO; GO:0061462; P:protein localization to lysosome; ISS:UniProtKB.
DR GO; GO:0050790; P:regulation of catalytic activity; IEA:GOC.
DR GO; GO:0008361; P:regulation of cell size; ISS:UniProtKB.
DR InterPro; IPR034601; LAMTOR4.
DR PANTHER; PTHR33967; PTHR33967; 1.
PE 1: Evidence at protein level;
KW Lysosome; Reference proteome.
FT CHAIN 1..120
FT /note="Ragulator complex protein LAMTOR4 homolog"
FT /id="PRO_0000325846"
FT REGION 93..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 19
FT /note="I -> V (in Ref. 3; AAL68349)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 120 AA; 13262 MW; CC13F3B5DE16F73E CRC64;
MLKMDREKLI VPNQIGYLIL KEDGAVLESG GDLKNDERSA NVIMGLLNLT ETIDESFMPS
SSCERITIDY EHHYYSICMS NRRIYIIKIS KSQNGVTTTT SSSSSNSVYN DASDSGAVLA
