LTOR4_MOUSE
ID LTOR4_MOUSE Reviewed; 99 AA.
AC Q8CF66; B7ZN18; Q8CEW4; Q8CF77;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Ragulator complex protein LAMTOR4;
DE AltName: Full=Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4;
DE Contains:
DE RecName: Full=Ragulator complex protein LAMTOR4, N-terminally processed;
GN Name=Lamtor4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: As part of the Ragulator complex it is involved in amino acid
CC sensing and activation of mTORC1, a signaling complex promoting cell
CC growth in response to growth factors, energy levels, and amino acids.
CC Activated by amino acids through a mechanism involving the lysosomal V-
CC ATPase, the Ragulator functions as a guanine nucleotide exchange factor
CC activating the small GTPases Rag. Activated Ragulator and Rag GTPases
CC function as a scaffold recruiting mTORC1 to lysosomes where it is in
CC turn activated (By similarity). {ECO:0000250|UniProtKB:Q0VGL1}.
CC -!- SUBUNIT: Part of the Ragulator complex composed of LAMTOR1, LAMTOR2,
CC LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer
CC that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer.
CC The Ragulator complex interacts with both the mTORC1 complex and
CC heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and
CC RRAGD; regulated by amino acid availability. The Ragulator complex
CC interacts with SLC38A9; the probable amino acid sensor. Component of
CC the lysosomal folliculin complex (LFC), composed of FLCN, FNIP1 (or
CC FNIP2), RRAGA/RagA or RRAGB/RagB GDP-bound, RRAGC/RagC or RRAGD/RagD
CC GTP-bound, and Ragulator. {ECO:0000250|UniProtKB:Q0VGL1}.
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000250|UniProtKB:Q0VGL1}.
CC -!- SIMILARITY: Belongs to the LAMTOR4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC24993.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC25020.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC25306.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK002488; BAC24993.1; ALT_INIT; mRNA.
DR EMBL; AK003086; BAC25020.1; ALT_INIT; mRNA.
DR EMBL; AK010598; BAC25306.1; ALT_INIT; mRNA.
DR EMBL; BC144958; AAI44959.1; -; mRNA.
DR CCDS; CCDS39342.1; -.
DR RefSeq; NP_001074577.1; NM_001081108.2.
DR AlphaFoldDB; Q8CF66; -.
DR SMR; Q8CF66; -.
DR BioGRID; 211211; 7.
DR ComplexPortal; CPX-4761; Ragulator complex.
DR CORUM; Q8CF66; -.
DR IntAct; Q8CF66; 1.
DR STRING; 10090.ENSMUSP00000056693; -.
DR PhosphoSitePlus; Q8CF66; -.
DR EPD; Q8CF66; -.
DR jPOST; Q8CF66; -.
DR MaxQB; Q8CF66; -.
DR PaxDb; Q8CF66; -.
DR PRIDE; Q8CF66; -.
DR ProteomicsDB; 292051; -.
DR TopDownProteomics; Q8CF66; -.
DR Antibodypedia; 16403; 66 antibodies from 16 providers.
DR Ensembl; ENSMUST00000062067; ENSMUSP00000056693; ENSMUSG00000050552.
DR GeneID; 66096; -.
DR KEGG; mmu:66096; -.
DR UCSC; uc009afd.2; mouse.
DR CTD; 389541; -.
DR MGI; MGI:1913346; Lamtor4.
DR VEuPathDB; HostDB:ENSMUSG00000050552; -.
DR eggNOG; ENOG502S3B2; Eukaryota.
DR GeneTree; ENSGT00390000016053; -.
DR HOGENOM; CLU_137556_1_0_1; -.
DR InParanoid; Q8CF66; -.
DR OMA; CRFRLHG; -.
DR OrthoDB; 1589714at2759; -.
DR PhylomeDB; Q8CF66; -.
DR TreeFam; TF324352; -.
DR Reactome; R-MMU-1632852; Macroautophagy.
DR Reactome; R-MMU-165159; MTOR signalling.
DR Reactome; R-MMU-166208; mTORC1-mediated signalling.
DR Reactome; R-MMU-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR Reactome; R-MMU-5628897; TP53 Regulates Metabolic Genes.
DR Reactome; R-MMU-8943724; Regulation of PTEN gene transcription.
DR Reactome; R-MMU-9639288; Amino acids regulate mTORC1.
DR BioGRID-ORCS; 66096; 19 hits in 73 CRISPR screens.
DR ChiTaRS; Lamtor4; mouse.
DR PRO; PR:Q8CF66; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8CF66; protein.
DR Bgee; ENSMUSG00000050552; Expressed in granulocyte and 255 other tissues.
DR Genevisible; Q8CF66; MM.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0031902; C:late endosome membrane; IC:ComplexPortal.
DR GO; GO:0005765; C:lysosomal membrane; IC:ComplexPortal.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0071986; C:Ragulator complex; ISS:UniProtKB.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISO:MGI.
DR GO; GO:0060090; F:molecular adaptor activity; ISO:MGI.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; ISS:UniProtKB.
DR GO; GO:0032008; P:positive regulation of TOR signaling; ISS:UniProtKB.
DR GO; GO:0061462; P:protein localization to lysosome; ISS:UniProtKB.
DR GO; GO:0008361; P:regulation of cell size; ISS:UniProtKB.
DR GO; GO:0038202; P:TORC1 signaling; IC:ComplexPortal.
DR InterPro; IPR034601; LAMTOR4.
DR PANTHER; PTHR33967; PTHR33967; 1.
PE 1: Evidence at protein level;
KW Acetylation; Lysosome; Reference proteome.
FT CHAIN 1..99
FT /note="Ragulator complex protein LAMTOR4"
FT /id="PRO_0000424499"
FT INIT_MET 1
FT /note="Removed; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q0VGL1"
FT CHAIN 2..99
FT /note="Ragulator complex protein LAMTOR4, N-terminally
FT processed"
FT /id="PRO_0000325842"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q0VGL1"
FT MOD_RES 2
FT /note="N-acetylthreonine; in Ragulator complex protein
FT LAMTOR4, N-terminally processed"
FT /evidence="ECO:0000250|UniProtKB:Q0VGL1"
FT CONFLICT 81
FT /note="G -> R (in Ref. 1; BAC24993)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 99 AA; 10678 MW; 177FF7B0CCF8BD9C CRC64;
MTSALTQGLE RIPDQLGYLV LSEGAVLASS GDLENDEQAA SAISELVSTA CGFQLHHGTN
IPFKRLSVVF GEHTLLVTVS GQRVFVVKRQ NRGREPIDV
