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LTP_VZVO
ID   LTP_VZVO                Reviewed;        2763 AA.
AC   Q4JQX9; Q4JQV3;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Large tegument protein deneddylase {ECO:0000255|HAMAP-Rule:MF_04044};
DE            EC=3.4.19.12 {ECO:0000255|HAMAP-Rule:MF_04044};
DE            EC=3.4.22.- {ECO:0000255|HAMAP-Rule:MF_04044};
GN   ORFNames=ORF22;
OS   Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=341980;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Human/Japan/P-Oka/1970, and
RC   Oka varicella vaccine Biken (V-Oka-Biken);
RX   PubMed=12388706; DOI=10.1128/jvi.76.22.11447-11459.2002;
RA   Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.;
RT   "Comparison of the complete DNA sequences of the Oka varicella vaccine and
RT   its parental virus.";
RL   J. Virol. 76:11447-11459(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oka varicella vaccine VarilRix (V-Oka-GSK), and
RC   Oka varicella vaccine Varivax (V-Oka-Merk);
RX   PubMed=18787000; DOI=10.1128/jvi.00777-08;
RA   Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M.,
RA   Vassilev V.;
RT   "Complete DNA sequences of two oka strain varicella-zoster virus genomes.";
RL   J. Virol. 82:11023-11044(2008).
CC   -!- FUNCTION: Large tegument protein that plays multiple roles in the viral
CC       cycle. During viral entry, remains associated with the capsid while
CC       most of the tegument is detached and participates in the capsid
CC       transport toward the host nucleus. Plays a role in the routing of the
CC       capsid at the nuclear pore complex and subsequent uncoating. Within the
CC       host nucleus, acts as a deneddylase and promotes the degradation of
CC       nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes
CC       nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These
CC       modifications prevent host cell cycle S-phase progression and create a
CC       favorable environment allowing efficient viral genome replication.
CC       Participates later in the secondary envelopment of capsids. Indeed,
CC       plays a linker role for the association of the outer viral tegument to
CC       the capsids together with the inner tegument protein.
CC       {ECO:0000255|HAMAP-Rule:MF_04044}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12; Evidence={ECO:0000255|HAMAP-Rule:MF_04044};
CC   -!- SUBUNIT: Interacts with host CUL1 and CUL4A; these interactions inhibit
CC       the E3 ligase activity of cullins. Interacts with inner tegument
CC       protein. Interacts with capsid vertex specific component CVC2.
CC       Interacts with the major capsid protein/MCP. {ECO:0000255|HAMAP-
CC       Rule:MF_04044}.
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000255|HAMAP-
CC       Rule:MF_04044}. Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04044}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04044}. Note=Tightly associated with
CC       the capsid. {ECO:0000255|HAMAP-Rule:MF_04044}.
CC   -!- SIMILARITY: Belongs to the herpesviridae large tegument protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04044}.
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DR   EMBL; AB097932; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AB097933; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ008355; AAY57708.1; -; Genomic_DNA.
DR   EMBL; DQ008354; AAY57637.1; -; Genomic_DNA.
DR   SMR; Q4JQX9; -.
DR   IntAct; Q4JQX9; 20.
DR   MINT; Q4JQX9; -.
DR   PRIDE; Q4JQX9; -.
DR   Proteomes; UP000002603; Genome.
DR   Proteomes; UP000008504; Genome.
DR   Proteomes; UP000008505; Genome.
DR   Proteomes; UP000008506; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019784; F:deNEDDylase activity; IEA:InterPro.
DR   GO; GO:0039648; P:modulation by virus of host protein ubiquitination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:InterPro.
DR   HAMAP; MF_04044; HSV_LTP; 1.
DR   InterPro; IPR005210; Herpes_LT_deneddylase.
DR   InterPro; IPR006928; Herpes_teg_USP.
DR   InterPro; IPR034702; HSV_LTP.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   Pfam; PF04843; Herpes_teg_N; 1.
DR   Pfam; PF03586; Herpes_UL36; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
DR   PROSITE; PS51521; HTUSP; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host nucleus; Host-virus interaction; Hydrolase;
KW   Modulation of host ubiquitin pathway by viral deubiquitinase;
KW   Modulation of host ubiquitin pathway by virus; Protease; Repeat;
KW   Thiol protease; Ubl conjugation pathway; Virion; Virion tegument.
FT   CHAIN           1..2763
FT                   /note="Large tegument protein deneddylase"
FT                   /id="PRO_0000385479"
FT   DOMAIN          12..237
FT                   /note="Peptidase C76"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   REPEAT          2455..2457
FT                   /note="1"
FT   REPEAT          2458..2460
FT                   /note="2"
FT   REPEAT          2461..2463
FT                   /note="3"
FT   REPEAT          2464..2466
FT                   /note="4"
FT   REPEAT          2467..2469
FT                   /note="5"
FT   REPEAT          2470..2472
FT                   /note="6"
FT   REPEAT          2473..2475
FT                   /note="7"
FT   REPEAT          2476..2478
FT                   /note="8"
FT   REGION          1..247
FT                   /note="Deubiquitination activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   REGION          495..523
FT                   /note="Interaction with inner tegument protein"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   REGION          2455..2478
FT                   /note="8 X 3 AA repeats of P-A/V-Q"
FT   REGION          2630..2651
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        32
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   SITE            19
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04044"
FT   VARIANT         2468
FT                   /note="A -> V (in strain: Oka varicella vaccine VarilRix
FT                   (V-Oka-GSK))"
SQ   SEQUENCE   2763 AA;  306336 MW;  C0E4F5AC0A599529 CRC64;
     MDIIPPIAVT VAGVGSRNQF DGALGPASGL SCLRTSLSFL HMTYAHGINA TLSSDMIDGC
     LQEGAAWTTD LSNMGRGVPD MCALVDLPNR ISYIKLGDTT STCCVLSRIY GDSHFFTVPD
     EGFMCTQIPA RAFFDDVWMG REESYTIITV DSTGMAIYRQ GNISFIFDPH GHGTIGQAVV
     VRVNTTDVYS YIASEYTHRP DNVESQWAAA LVFFVTANDG PVSEEALSSA VTLIYGSCDT
     YFTDEQYCEK LVTAQHPLLL SPPNSTTIVL NKSSIVPLHQ NVGESVSLEA TLHSTLTNTV
     ALDPRCSYSE VDPWHAVLET TSTGSGVLDC RRRRRPSWTP PSSEENLACI DDGLVNNTHS
     TDNLHKPAKK VLKFKPTVDV PDKTQVAHVL PRLREVANTP DVVLNVSNVD TPESSPTFSR
     NMNVGSSLKD RKPFLFEQSG DVNMVVEKLL QHGHEISNGY VQNAVGTLDT VITGHTNVPI
     WVTRPLVMPD EKDPLELFIN LTILRLTGFV VENGTRTHHG ATSVVSDFIG PLGEILTGFP
     SAAELIRVTS LILTNMPGAE YAIKTVLRKK CTIGMLIIAK FGLVAMRVQD TTGALHAELD
     VLEADLGGSS PIDLYSRLST GLISILNSPI ISHPGLFAEL IPTRTGSLSE RIRLLCELVS
     ARETRYMREH TALVSSVKAL ENALRSTRNK IDAIQIPEVP QEPPEETDIP PEELIRRVYE
     IRSEVTMLLT SAVTEYFTRG VLYSTRALIA EQSPRRFRVA TASTAPIQRL LDSLPEFDAK
     LTAIISSLSI HPPPETIQNL PVVSLLKELI KEGEDLNTDT ALVSWLSVVG EAQTAGYLSR
     REFDELSRTI KTINTRATQR ASAEAELSCF NTLSAAVDQA VKDYETYNNG EVKYPEITRD
     DLLATIVRAT DDLVRQIKIL SDPMIQSGLQ PSIKRRLETR LKEVQTYANE ARTTQDTIKS
     RKQAAYNKLG GLLRPVTGFV GLRAAVDLLP ELASELDVQG ALVNLRTKVL EAPVEIRSQL
     TGDFWALFNQ YRDILEHPGN ARTSVLGGLG ACFTAIIEIV PIPTEYRPSL LAFFGDVADV
     LASDIATVST NPESESAINA VVATLSKATL VSSTVPALSF VLSLYKKYQA LQQEITNTHK
     LTELQKQLGD DFSTLAVSSG HLKFISSSNV DDYEINDAIL SIQTNVHALM DTVKLVEVEL
     QKLPPHCIAG TSTLSRVVKD LHKLVTMAHE KKEQAKVLIT DCERAHKQQT TRVLYERWTR
     DIIACLEAME TRHVFNGTEL ARLRDMAAAG GFDIHAVYPQ ARQVVAACET TAVTALDTVF
     RHNPHTPENT NIPPPLALLR GLTWFDDFSI TAPVFTVMFP GVSIEGLLLL MRIRAVVLLS
     ADTSINGIPN YRDMILRTSG DLLQIPALAG YVDFYTRSYD QFITESVTLS ELRADIRQAA
     GAKLTEANKA LEEVTHVRAH ETAKLALKEG VFITLPSEGL LIRAIEYFTT FDHKRFIGTA
     YERVLQTMVD RDLKEANAEL AQFRMVCQAT KNRAIQILQN IVDTANATEQ QEDVDFTNLK
     TLLKLTPPPK TIALAIDRST SVQDIVTQFA LLLGRLEEET GTLDIQAVDW MYQARNIIDS
     HPLSVRIDGT GPLHTYKDRV DKLYALRTKL DLLRRRIETG EVTWDDAWTT FKRETGDMLA
     SGDTYATSVD SIKALQASAS VVDMLCSEPE FFLLPVETKN RLQKKQQERK TALDVVLQKQ
     RQFEETASRL RALIERIPTE SDHDVLRMLL HDFDQFTHLP IWIKTQYMTF RNLLMVRLGL
     YASYAEIFPP ASPNGVFAPI PAMSGVCLED QSRCIRARVA AFMGEASVVQ TFREARSSID
     ALFGKNLTFY LDTDGVPLRY RVCYKSVGVK LGTMLCSQGG LSLRPALPDE GIVEETTLSA
     LRVANEVNEL RIEYESAIKS GFSAFSTFVR HRHAEWGKTN ARRAIAEIYA GLITTTLTRQ
     YGVHWDKLIY SFEKHHLTSV MGNGLTKPIQ RRGDVRVLEL TLSDIVTILV ATTPVHLLNF
     ARLDLIKQHE YMARTLRPVI EAAFRGRLLV RSLDGDPKGN ARAFFNAAPS KHKLPLALGS
     NQDPTGGRIF AFRMADWKLV KMPQKITDPF APWQLSPPPG VKANVDAVTR IMATDRLATI
     TVLGRMCLPP ISLVSMWNTL QPEEFAYRTQ DDVDIIVDAR LDLSSTLNAR FDTAPSNTTL
     EWNTDRKVIT DAYIQTGATT VFTVTGAAPT HVSNVTAFDI ATTAILFGAP LVIAMELTSV
     FSQNSGLTLG LKLFDSRHMA TDSGISSAVS PDIVSWGLRL LHMDPHPIEN ACLIVQLEKL
     SALIANKPLT NNPPCLLLLD EHMNPSYVLW ERKDSIPAPD YVVFWGPESL IDLPYIDSDE
     DSFPSCPDDP FYSQIIAGYA PQGPPNLDTT DFYPTEPLFK SPVQVVRSSK CKKMPVQPVQ
     PAQPVQPAQP AQPVQPAQPI EPGTQIVVQN FKKPQSVKTT LSQKDIPLYV ETESETAVLI
     PKQLTTSIKT TVCKSITPPN NQLSDWKNNP QQNQTLNQAF NKPILEITSI PTDDSISYRT
     WIEKSNQTQK RHQNDPRMYN SKTVFHPVNN QLPSWVDTAA DAPQTDLLTN YKTRQPSPNF
     PRDVHTWGVS SNPFNSPNRD LYESDFSEPS DGYSSESENS IVLSLDEHRS CRVPRHVRVV
     NADVVTGRRY VRGTALGALA LLSQACRRMI DNVRYTRKLL MDHTEDIFQG LGYVKLLLDG
     TYI
 
 
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