LTX2C_LACTA
ID LTX2C_LACTA Reviewed; 108 AA.
AC B3EWF6; K7WMQ9;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 1.
DT 25-MAY-2022, entry version 16.
DE RecName: Full=Latartoxin-2c {ECO:0000303|PubMed:23088912};
DE Short=LtTx-2c {ECO:0000303|PubMed:23088912};
DE Flags: Precursor;
OS Lachesana tarabaevi (Spider).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC Lachesana.
OX NCBI_TaxID=379576;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-107, FUNCTION,
RP SUBCELLULAR LOCATION, DISULFIDE BONDS, MASS SPECTROMETRY, TOXIC DOSE, AND
RP AMIDATION AT ILE-107.
RC TISSUE=Venom, and Venom gland;
RX PubMed=23088912; DOI=10.1016/j.bbamem.2012.10.014;
RA Kuzmenkov A.I., Fedorova I.M., Vassilevski A.A., Grishin E.V.;
RT "Cysteine-rich toxins from Lachesana tarabaevi spider venom with
RT amphiphilic C-terminal segments.";
RL Biochim. Biophys. Acta 1828:724-731(2013).
RN [2]
RP SUBCELLULAR LOCATION, PQM MOTIF, AND MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=27287558; DOI=10.1042/bcj20160436;
RA Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA Vassilevski A.A.;
RT "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL Biochem. J. 473:2495-2506(2016).
CC -!- FUNCTION: Insect toxin. {ECO:0000269|PubMed:23088912}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23088912,
CC ECO:0000269|PubMed:27287558}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:23088912}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- PTM: Contains 4 disulfide bonds. {ECO:0000269|PubMed:23088912}.
CC -!- PTM: Cleavage of the propeptide depends on the processing quadruplet
CC motif (XXXR, with at least one of X being E).
CC {ECO:0000303|PubMed:27287558}.
CC -!- MASS SPECTROMETRY: Mass=7669.1; Mass_error=0.5; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:23088912};
CC -!- MASS SPECTROMETRY: Mass=7669.7; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:27287558};
CC -!- TOXIC DOSE: LD(50) is 50 ug/g in flesh fly larvae (S.carnaria).
CC {ECO:0000269|PubMed:23088912}.
CC -!- TOXIC DOSE: LD(50) is 50 ug/g in house crickets (Acheta domesticus).
CC {ECO:0000269|PubMed:23088912}.
CC -!- SIMILARITY: Belongs to the neurotoxin 19 (CSTX) family. 11 (latartoxin)
CC subfamily. {ECO:0000255}.
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DR EMBL; JQ513647; AFX65332.1; -; mRNA.
DR AlphaFoldDB; B3EWF6; -.
DR SMR; B3EWF6; -.
DR ArachnoServer; AS001867; U2-zodatoxin-Lt2c.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Disulfide bond; Knottin; Secreted;
KW Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..42
FT /note="Removed in mature form"
FT /evidence="ECO:0000269|PubMed:23088912"
FT /id="PRO_0000421849"
FT PEPTIDE 43..107
FT /note="Latartoxin-2c"
FT /id="PRO_0000421850"
FT MOTIF 39..42
FT /note="Processing quadruplet motif"
FT /evidence="ECO:0000303|PubMed:27287558"
FT MOD_RES 107
FT /note="Isoleucine amide"
FT /evidence="ECO:0000269|PubMed:23088912"
FT DISULFID 44..61
FT /evidence="ECO:0000250|UniProtKB:P58604"
FT DISULFID 51..72
FT /evidence="ECO:0000250|UniProtKB:P58604"
FT DISULFID 60..84
FT /evidence="ECO:0000250|UniProtKB:P58604"
FT DISULFID 74..82
FT /evidence="ECO:0000250|UniProtKB:P58604"
SQ SEQUENCE 108 AA; 12617 MW; 5011538231BF300F CRC64;
MKVLVITALC FILLQNVLGE DTYEDLQNYI ENLINENQDE ARECVPLEND CTKLKYSNPC
CKDEKKKYQY KCSCIVDKTE QCTCQRKETV EKMMKGMKYI KNLGKKIG