LUB1_SCHPO
ID LUB1_SCHPO Reviewed; 718 AA.
AC O94289;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Ubiquitin homeostasis protein lub1;
GN Name=lub1; ORFNames=SPBC887.04c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP FUNCTION, INTERACTION WITH CDC48, AND SUBCELLULAR LOCATION.
RX PubMed=14993272; DOI=10.1128/mcb.24.6.2324-2331.2004;
RA Ogiso Y., Sugiura R., Kamo T., Yanagiya S., Lu Y., Okazaki K., Shuntoh H.,
RA Kuno T.;
RT "Lub1 participates in ubiquitin homeostasis and stress response via
RT maintenance of cellular ubiquitin contents in fission yeast.";
RL Mol. Cell. Biol. 24:2324-2331(2004).
CC -!- FUNCTION: Acts as a negative regulator of vacuole-dependent ubiquitin
CC degradation. {ECO:0000269|PubMed:14993272}.
CC -!- SUBUNIT: Interacts with cdc48. {ECO:0000269|PubMed:14993272}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14993272}. Cytoplasm
CC {ECO:0000269|PubMed:14993272}.
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DR EMBL; CU329671; CAA21889.2; -; Genomic_DNA.
DR PIR; T40729; T40729.
DR RefSeq; NP_596478.2; NM_001022398.2.
DR AlphaFoldDB; O94289; -.
DR SMR; O94289; -.
DR BioGRID; 277722; 175.
DR STRING; 4896.SPBC887.04c.1; -.
DR iPTMnet; O94289; -.
DR MaxQB; O94289; -.
DR PaxDb; O94289; -.
DR EnsemblFungi; SPBC887.04c.1; SPBC887.04c.1:pep; SPBC887.04c.
DR GeneID; 2541208; -.
DR KEGG; spo:SPBC887.04c; -.
DR PomBase; SPBC887.04c; lub1.
DR VEuPathDB; FungiDB:SPBC887.04c; -.
DR eggNOG; KOG0301; Eukaryota.
DR HOGENOM; CLU_011791_2_0_1; -.
DR InParanoid; O94289; -.
DR OMA; HNVCALD; -.
DR Reactome; R-SPO-8951664; Neddylation.
DR Reactome; R-SPO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:O94289; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0043130; F:ubiquitin binding; ISO:PomBase.
DR GO; GO:0006281; P:DNA repair; ISS:PomBase.
DR GO; GO:0016574; P:histone ubiquitination; NAS:PomBase.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IMP:PomBase.
DR GO; GO:0010992; P:ubiquitin recycling; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.10.20.870; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR015155; PFU.
DR InterPro; IPR038122; PFU_sf.
DR InterPro; IPR013535; PUL_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF09070; PFU; 1.
DR Pfam; PF08324; PUL; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51394; PFU; 1.
DR PROSITE; PS51396; PUL; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway;
KW WD repeat.
FT CHAIN 1..718
FT /note="Ubiquitin homeostasis protein lub1"
FT /id="PRO_0000051070"
FT REPEAT 12..50
FT /note="WD 1"
FT REPEAT 54..98
FT /note="WD 2"
FT REPEAT 101..139
FT /note="WD 3"
FT REPEAT 140..178
FT /note="WD 4"
FT REPEAT 180..217
FT /note="WD 5"
FT REPEAT 218..257
FT /note="WD 6"
FT REPEAT 259..296
FT /note="WD 7"
FT DOMAIN 353..448
FT /note="PFU"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00727"
FT DOMAIN 462..717
FT /note="PUL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00729"
SQ SEQUENCE 718 AA; 79222 MW; DC74201E79CDB6C2 CRC64;
MTSYELSREL GGHKQDVRGV CSISNELIGS ASRDGTYSVW EQINGEWTPH FYENHEGFVN
CVCYVPAIDK NSRGYIFSGG QDKCGILQEV GTNSPSYYLF GHESNICSAS ALNSETIITG
SWDSTARVWA LGQCKYVLKG HQSSVWAVLA LGEDIFITGS ADKLIKIWNG EKLVKSILAH
NDCVRSLCQI PGGFASCSND GVIKLWTSDG EFLYELHGHT SFVYSLTYIH NQQLIASCGE
DRTIRIWKGK ECLQCITLPT TSVWSVSSLP NGDLVCGSSD GFVRIFTVDK VRVAPTEVLK
NFEERVSQFA ISSQEVGDIK KGSLPGLEIL SKPGKADGDV VMVRVNNDVE AYQWSQKENE
WKKIGQVVDA VGNNRKQLFE GKEYDYVFDV DVADGQAPLK LPYNATENPY QAANRFLELN
QLPLSYTDEV VKFIEKNTQG HSLESKKEPN LESQSSNKIK TTIFPVSQLL FSNANVPAMC
QRLRSLNNTK SNPLPAKSID SLERALSSKK ITDTEKNELL ETCLSILDSW SLAERFPALD
ALRLLAINSS SDLAPIFLEV FSRVVKSVPS SGNFESINVM LALRGLSNVV PNITDAEGVS
KLMDCLTSTV PQASSAKDFK IAFATLAMNL SILLIQLNLE NTGIELLSIL FSFLDDPSPD
NEAFYRALMA LGTLCTVPDI ALAASQIYHA QSIVHGIAER FSQEMRFVDA EKQILSLF
