ID   LUCA_MYCTU              Reviewed;         254 AA.
AC   O69690; F2GFU5; I6XI20; Q7D500;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Lipid uptake coordinator A {ECO:0000303|PubMed:28708968};
GN   Name=lucA {ECO:0000303|PubMed:28708968};
GN   OrderedLocusNames=Rv3723 {ECO:0000312|EMBL:CCP46549.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   FUNCTION, INTERACTION WITH MCE1 AND MCE4, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=28708968; DOI=10.7554/elife.26969;
RA   Nazarova E.V., Montague C.R., La T., Wilburn K.M., Sukumar N., Lee W.,
RA   Caldwell S., Russell D.G., VanderVen B.C.;
RT   "Rv3723/LucA coordinates fatty acid and cholesterol uptake in Mycobacterium
RT   tuberculosis.";
RL   Elife 6:0-0(2017).
CC   -!- FUNCTION: Required for the import of both fatty acids and cholesterol
CC       during growth in macrophages and in axenic culture. Facilitates the
CC       uptake of these lipids by stabilizing protein subunits of the Mce1 and
CC       Mce4 multi-subunit transporters, which transport fatty acids and
CC       cholesterol, respectively. Required for full virulence in vivo.
CC       {ECO:0000269|PubMed:28708968}.
CC   -!- SUBUNIT: Interacts with the Mce1 and Mce4 accessory subunits
CC       Rv0199/OmamA, Rv0177/Mam1C and Rv3492c/Mam4B.
CC       {ECO:0000269|PubMed:28708968}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28708968};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutant is unable to utilize both fatty acids and
CC       cholesterol during infection in macrophages.
CC       {ECO:0000269|PubMed:28708968}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP46549.1; -; Genomic_DNA.
DR   RefSeq; NP_218240.1; NC_000962.3.
DR   RefSeq; WP_003420421.1; NZ_NVQJ01000009.1.
DR   AlphaFoldDB; O69690; -.
DR   STRING; 83332.Rv3723; -.
DR   PaxDb; O69690; -.
DR   PRIDE; O69690; -.
DR   GeneID; 885791; -.
DR   KEGG; mtu:Rv3723; -.
DR   PATRIC; fig|83332.111.peg.4139; -.
DR   TubercuList; Rv3723; -.
DR   eggNOG; ENOG5031BRQ; Bacteria.
DR   OMA; IWLSLDA; -.
DR   PHI-base; PHI:7268; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..254
FT                   /note="Lipid uptake coordinator A"
FT                   /id="PRO_0000441801"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          143..254
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        171..194
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        231..245
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   254 AA;  27367 MW;  263D25A2797C5BA8 CRC64;
     MGRKVAVLWH ASFSIGAGVL YFYFVLPRWP ELMGDTGHSL GTGLRIATGA LVGLAALPVV
     FTLLRTRKPE LGTPQLALSM RIWSIMAHVL AGALIVGTAI SEVWLSLDAA GQWLFGIYGA
     AAAIAVLGFF GFYLSFVAEL PPPPPKPLKP KKPKQRRLRR KKTAKGDEAE PEAAEEAENT
     ELAAQEDEEA VEAPPESIES PGGEPESATR EAPAAETATA EEPRGGLRNR RPTGKTSHRR
     RRTRSGVQVA KVDE