ID   LUCFE_LUCCU             Reviewed;          40 AA.
AC   B3EWY5;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2013, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Lucifensin {ECO:0000250|UniProtKB:P86471};
DE   AltName: Full=Lucifensin II {ECO:0000303|PubMed:23802451};
OS   Lucilia cuprina (Green bottle fly) (Australian sheep blowfly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Calliphoridae; Luciliinae; Lucilia.
OX   NCBI_TaxID=7375;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND DISULFIDE BONDS.
RC   TISSUE=Larval hemolymph {ECO:0000303|PubMed:23802451};
RX   PubMed=23802451; DOI=10.1603/me12208;
RA   El Shazely B., Veverka V., Fucik V., Voburka Z., Zdarek J., Cerovsky V.;
RT   "Lucifensin II, a defensin of medicinal maggots of the blowfly Lucilia
RT   cuprina (Diptera: Calliphoridae).";
RL   J. Med. Entomol. 50:571-578(2013).
CC   -!- FUNCTION: Shows strong antibacterial activity against the Gram-positive
CC       bacterium M.luteus (PubMed:23802451). Also shows antibacterial activity
CC       against the Gram-positive bacteria E.fecalis, S.aureus, S.carnosus,
CC       S.pneumoniae and S.pyogenes and against a number of methicillin-
CC       resistant S.aureus and glycopeptide-intermediate S.aureus isolates (By
CC       similarity). Does not show antibacterial activity against Gram-negative
CC       bacteria or antifungal activity against C.utilis (By similarity). Shows
CC       slight antifungal activity against C.albicans (By similarity).
CC       {ECO:0000250|UniProtKB:P86471, ECO:0000269|PubMed:23802451}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC       ECO:0000269|PubMed:23802451}.
CC   -!- TISSUE SPECIFICITY: Larval fat body, hemolymph and salivary glands (at
CC       protein level). {ECO:0000269|PubMed:23802451}.
CC   -!- PTM: The disulfide bonds are essential for antimicrobial activity.
CC       {ECO:0000250|UniProtKB:P86471}.
CC   -!- MASS SPECTROMETRY: Mass=4127.93; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:23802451};
CC   -!- MISCELLANEOUS: During maggot debridement therapy which involves the
CC       controlled application of cultured sterile larvae to an infected
CC       chronic non-healing wound, one of the key factors which protects larvae
CC       against the infectious wound environment and contributes to wound
CC       healing through its antimicrobial activity.
CC       {ECO:0000269|PubMed:23802451}.
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   AlphaFoldDB; B3EWY5; -.
DR   BMRB; B3EWY5; -.
DR   SMR; B3EWY5; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR017982; Defensin_insect.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   PRINTS; PR00271; DEFENSIN.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..40
FT                   /note="Lucifensin"
FT                   /evidence="ECO:0000269|PubMed:23802451"
FT                   /id="PRO_0000421470"
FT   DISULFID        3..30
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT                   ECO:0000269|PubMed:23802451"
FT   DISULFID        16..36
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT                   ECO:0000269|PubMed:23802451"
FT   DISULFID        20..38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT                   ECO:0000269|PubMed:23802451"
SQ   SEQUENCE   40 AA;  4137 MW;  077BFED58F9586FD CRC64;
     ATCDLLSGTG IKHSACAAHC LLRGNRGGYC NGRAICVCRN