ID   LUKDV_STAAS             Reviewed;         327 AA.
AC   Q6G8A9;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Leucotoxin LukDv;
DE   AltName: Full=Variant of LukD;
DE   Flags: Precursor;
GN   Name=lukDv; Synonyms=hlgB; OrderedLocusNames=SAS1748;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
RN   [2]
RP   SIMILARITY TO LUKDV.
RX   PubMed=15170101; DOI=10.1271/bbb.68.981;
RA   Kaneko J., Kamio Y.;
RT   "Bacterial two-component and hetero-heptameric pore-forming cytolytic
RT   toxins: structures, pore-forming mechanism, and organization of the
RT   genes.";
RL   Biosci. Biotechnol. Biochem. 68:981-1003(2004).
CC   -!- FUNCTION: Part of a bi-component leucotoxin that acts by forming pores
CC       in the membrane of the target cells. The activity of LukEv-LukDv to
CC       rabbit leukocytes is similar to that of the Panton-Valentine leucocidin
CC       (PVL). LukEv-LukDv is hemolytic to rabbit red blood cells although the
CC       activity is only 8% of gamma-hemolysin (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Toxicity requires sequential binding and synergistic
CC       association of a class S and a class F component which form
CC       heterooligomeric complexes. LukEv (class S) associates with LukDv
CC       (class F) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the aerolysin family. {ECO:0000305}.
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DR   EMBL; BX571857; CAG43552.1; -; Genomic_DNA.
DR   RefSeq; WP_000782464.1; NC_002953.3.
DR   AlphaFoldDB; Q6G8A9; -.
DR   SMR; Q6G8A9; -.
DR   ABCD; Q6G8A9; 1 sequenced antibody.
DR   KEGG; sas:SAS1748; -.
DR   HOGENOM; CLU_055394_0_1_9; -.
DR   OMA; DYSQFYW; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR003963; Bi-component_toxin_staph.
DR   InterPro; IPR016183; Leukocidin/Hemolysin_toxin.
DR   InterPro; IPR036435; Leukocidin/porin_MspA_sf.
DR   Pfam; PF07968; Leukocidin; 1.
DR   PRINTS; PR01468; BICOMPNTOXIN.
DR   SUPFAM; SSF56959; SSF56959; 1.
DR   TIGRFAMs; TIGR01002; hlyII; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Hemolysis; Secreted; Signal; Toxin; Virulence.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..327
FT                   /note="Leucotoxin LukDv"
FT                   /id="PRO_0000045219"
SQ   SEQUENCE   327 AA;  36889 MW;  3807DB6421ACDAAA CRC64;
     MKMKKLVKSS VASSIALLLL SNTVDAAQHI TPVSEKKVDD KITLYKTTAT SDNDKLNISQ
     ILTFNFIKDK SYDKDTLVLK AAGNINSGYK KPNPKDYNYS QFYWGGKYNV SVSSESNDAV
     NVVDYAPKNQ NEEFQVQQTL GYSYGGDINI SNGLSGGLNG SKSFSETINY KQESYRTTID
     RKTNHKSIGW GVEAHKIMNN GWGPYGRDSY DPTYGNELFL GGRQSSSNAG QNFLPTHQMP
     LLARGNFNPE FISVLSHKQN DTKKSKIKVT YQREMDRYTN QWNRLHWVGN NYKNQNTVTF
     TSTYEVDWQN HTVKLIGTDS KETNPGV