ID   LUKD_STAAU              Reviewed;         327 AA.
AC   O54082;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Leucotoxin LukD;
DE   Flags: Precursor;
GN   Name=lukD;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-44, FUNCTION,
RP   SUBUNIT, INDUCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=Newman;
RX   PubMed=9781679; DOI=10.1016/s0014-5793(98)01130-2;
RA   Gravet A., Colin D.A., Keller D., Giradot R., Monteil H., Prevost G.;
RT   "Characterization of a novel structural member, LukE-LukD, of the bi-
RT   component staphylococcal leucotoxins family.";
RL   FEBS Lett. 436:202-208(1998).
CC   -!- FUNCTION: Part of a bi-component leucotoxin that acts by forming pores
CC       in the membrane of the target cells. LukE-LukD is as effective as the
CC       Panton-Valentine leucocidin (PVL) for inducing dermonecrosis when
CC       injected in the rabbit skin, but not hemolytic and poorly leucotoxic on
CC       human blood cells compared to other leucotoxins expressed by S.aureus.
CC       HlgA-LukD is a Ca(2+) channel inducer. {ECO:0000269|PubMed:9781679}.
CC   -!- SUBUNIT: Toxicity requires sequential binding and synergistic
CC       association of a class S and a class F component which form
CC       heterooligomeric complexes (By similarity). LukE (class S) associates
CC       with LukD (class F). LukD can also associate with HlgA. {ECO:0000250,
CC       ECO:0000269|PubMed:9781679}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9781679}.
CC   -!- INDUCTION: Expressed at the late exponential growth. Is cotranscribed
CC       with lukE. {ECO:0000269|PubMed:9781679}.
CC   -!- SIMILARITY: Belongs to the aerolysin family. {ECO:0000305}.
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DR   EMBL; Y13225; CAA73668.1; -; Genomic_DNA.
DR   AlphaFoldDB; O54082; -.
DR   SMR; O54082; -.
DR   PRIDE; O54082; -.
DR   ABCD; O54082; 6 sequenced antibodies.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0051715; P:cytolysis in another organism; IEA:InterPro.
DR   InterPro; IPR003963; Bi-component_toxin_staph.
DR   InterPro; IPR016183; Leukocidin/Hemolysin_toxin.
DR   InterPro; IPR036435; Leukocidin/porin_MspA_sf.
DR   Pfam; PF07968; Leukocidin; 1.
DR   PRINTS; PR01468; BICOMPNTOXIN.
DR   SUPFAM; SSF56959; SSF56959; 1.
DR   TIGRFAMs; TIGR01002; hlyII; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Direct protein sequencing; Secreted; Signal; Toxin; Virulence.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000269|PubMed:9781679"
FT   CHAIN           27..327
FT                   /note="Leucotoxin LukD"
FT                   /id="PRO_5000147159"
SQ   SEQUENCE   327 AA;  36876 MW;  6CC5677521C3CD24 CRC64;
     MKIEKLGKSS VASSIALLLL SNTVDAAQNI TPKREKKVDD KITLYKTTAT SDNDKLNIFQ
     ILTFNFIKDK SYDKDTLVLK AAGNINSGYK NSNPKDYNYS QFYWGGKYNV SVSSESNDAV
     NVVDYAPKNQ NEEFQVQQTL GYSYGGDINI SNGLSGGLNG SKSFSETINY KQESYRTTID
     RKTNHKSIGW GVEAHKIMNN GWGPYGRDSY DPTYGNELFL GGDKSSSNAG QNFLPTHQIP
     LLARGNFNPE FISVLSHKLF DTKKSKIKVT YQREMDRYTN QWNRSHWVGN NYKNQNTVTF
     TSTYEVDWQN ILLKLIGTDS KETNPGV