ID   LUKF_STAAU              Reviewed;         323 AA.
AC   P31715;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Leukocidin-F subunit;
DE   AltName: Full=Gamma-hemolysin, H-gamma-I subunit;
DE   Flags: Precursor;
GN   Name=lukF;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 26-66.
RC   STRAIN=MRSA NO. 4;
RX   PubMed=1575738; DOI=10.1016/0006-291x(92)90637-z;
RA   Rahman A., Nariya H., Izaki K., Kato I., Kamio Y.;
RT   "Molecular cloning and nucleotide sequence of leukocidin F-component gene
RT   (lukF) from methicillin resistant Staphylococcus aureus.";
RL   Biochem. Biophys. Res. Commun. 184:640-646(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 27-85 AND 322-323.
RC   STRAIN=RIMD 310925;
RX   PubMed=8467905; DOI=10.1016/0014-5793(93)80611-w;
RA   Kamio Y., Rahman A., Nariya H., Ozawa T., Izaki K.;
RT   "The two Staphylococcal bi-component toxins, leukocidin and gamma-
RT   hemolysin, share one component in common.";
RL   FEBS Lett. 321:15-18(1993).
CC   -!- FUNCTION: Leukocidin causes cytotoxic changes in polymorphonuclear
CC       leukocytes. Gamma-hemolysin causes hemolysis in red blood cells.
CC   -!- SUBUNIT: Leukocidin consists of two protein components: F and S; gamma-
CC       hemolysin consists of two protein components (H-gamma-I which is
CC       identical to F and H-gamma-II).
CC   -!- SIMILARITY: Belongs to the aerolysin family. {ECO:0000305}.
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DR   EMBL; S65052; AAC60446.1; -; Genomic_DNA.
DR   PIR; JN0627; JN0627.
DR   PIR; JQ1530; JQ1530.
DR   AlphaFoldDB; P31715; -.
DR   SMR; P31715; -.
DR   ABCD; P31715; 7 sequenced antibodies.
DR   PRO; PR:P31715; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR003963; Bi-component_toxin_staph.
DR   InterPro; IPR016183; Leukocidin/Hemolysin_toxin.
DR   InterPro; IPR036435; Leukocidin/porin_MspA_sf.
DR   Pfam; PF07968; Leukocidin; 1.
DR   PRINTS; PR01468; BICOMPNTOXIN.
DR   SUPFAM; SSF56959; SSF56959; 1.
DR   TIGRFAMs; TIGR01002; hlyII; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Direct protein sequencing; Hemolysis; Signal; Toxin; Virulence.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:1575738"
FT   CHAIN           26..323
FT                   /note="Leukocidin-F subunit"
FT                   /id="PRO_0000018427"
SQ   SEQUENCE   323 AA;  36567 MW;  A5951CF2AAB6C7E8 CRC64;
     MKMNKLVKSS VATSMALLLL SGTANAEGKI TPVSVKKVDD KVTLYKTTAT ADSDKFKISQ
     ILTFNFIKDK SYDKDTLVLK ATGNINSGFV KPNPNDYDFS KLYWGAKYNV SISSQSNDSV
     NAVDYAPKNQ NEEFQVQNTL GYTFGGDISI SNGLSGGLNG NTAFSETINY KQESYRTLSR
     NTNYKNVGWG VEAHKIMNGW GPYGRDSFHP TYGNELFLAG RQSSAYAGQN FIAQHQMPLL
     SRSNFNPEFL SVLSHRQDRA KKSKITVTYQ REMDLYQIRW NGFYWAGANY KNFKTRTFKS
     TYEIDWENHK VKLLDTKETE NNK