LUP3_ARATH
ID LUP3_ARATH Reviewed; 769 AA.
AC P0C8Y0; O64553;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Camelliol C synthase;
DE EC=5.4.99.38;
DE AltName: Full=Lupeol synthase 3;
DE Short=AtLUP3;
GN Name=CAMS1; Synonyms=LUP3; OrderedLocusNames=At1g78955;
GN ORFNames=YUP8H12R.44;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=11247608; DOI=10.1023/a:1006476123930;
RA Husselstein-Muller T., Schaller H., Benveniste P.;
RT "Molecular cloning and expression in yeast of 2,3-oxidosqualene-
RT triterpenoid cyclases from Arabidopsis thaliana.";
RL Plant Mol. Biol. 45:75-92(2001).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=17985917; DOI=10.1021/ol702399g;
RA Kolesnikova M.D., Wilson W.K., Lynch D.A., Obermeyer A.C., Matsuda S.P.T.;
RT "Arabidopsis camelliol C synthase evolved from enzymes that make
RT pentacycles.";
RL Org. Lett. 9:5223-5226(2007).
CC -!- FUNCTION: Converts oxidosqualene to camelliol C. Minor production of
CC achilleol and beta-amyrin. {ECO:0000269|PubMed:17985917}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = camelliol C; Xref=Rhea:RHEA:31011,
CC ChEBI:CHEBI:15441, ChEBI:CHEBI:62452; EC=5.4.99.38;
CC Evidence={ECO:0000269|PubMed:17985917};
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; AC002986; AAC17080.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36184.1; -; Genomic_DNA.
DR PIR; T01060; T01060.
DR RefSeq; NP_683508.1; NM_148667.2.
DR AlphaFoldDB; P0C8Y0; -.
DR SMR; P0C8Y0; -.
DR STRING; 3702.AT1G78955.1; -.
DR PaxDb; P0C8Y0; -.
DR PRIDE; P0C8Y0; -.
DR ProteomicsDB; 238532; -.
DR EnsemblPlants; AT1G78955.1; AT1G78955.1; AT1G78955.
DR GeneID; 844235; -.
DR Gramene; AT1G78955.1; AT1G78955.1; AT1G78955.
DR KEGG; ath:AT1G78955; -.
DR Araport; AT1G78955; -.
DR TAIR; locus:504956092; AT1G78955.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_009074_2_0_1; -.
DR InParanoid; P0C8Y0; -.
DR OrthoDB; 365003at2759; -.
DR PhylomeDB; P0C8Y0; -.
DR BioCyc; ARA:AT1G78955-MON; -.
DR BioCyc; MetaCyc:AT1G78955-MON; -.
DR BRENDA; 5.4.99.38; 399.
DR PRO; PR:P0C8Y0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P0C8Y0; baseline and differential.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0042300; F:beta-amyrin synthase activity; IDA:TAIR.
DR GO; GO:0090438; F:camelliol C synthase activity; IDA:TAIR.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IBA:GO_Central.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome; Repeat.
FT CHAIN 1..769
FT /note="Camelliol C synthase"
FT /id="PRO_0000366133"
FT REPEAT 149..190
FT /note="PFTB 1"
FT REPEAT 641..682
FT /note="PFTB 2"
FT ACT_SITE 486
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 769 AA; 88156 MW; 30B7372DD5703C60 CRC64;
MWKLKIANGN KEEPYLFSTN NFLGRQTWEF DPDAGTVEEL AAVEEARRKF YDDRFRVKAS
SDLIWRMQFL KEKKFEQVIP PAKVEDANNI TSEIATNALR KGVNFLSALQ ASDGHWPAEN
AGPLFFLPPL VFCLYVTGHL HEIFTQDHRR EVLRYIYCHQ NEDGGWGLHI EGNSTMFCTT
LNYICMRILG EGPNGGPGNA CKRARDWILD HGGATYIPSW GKTWLSILGV FDWSGSNPMP
PEFWILPSFL PIHPAKMWCY CRLVYMPMSY LYGKRFVGPI SPLILQLREE IYLQPYAKIN
WNRARHLCAK EDAYCPHPQI QDVIWNCLYI FTEPFLACWP FNKLLREKAL GVAMKHIHYE
DENSRYITIG CVEKALCMLA CWVEDPNGIH FKKHLLRISD YLWIAEDGMK MQSFGSQLWD
SGFALQALVA SNLVNEIPDV LRRGYDFLKN SQVRENPSGD FTNMYRHISK GSWTFSDRDH
GWQASDCTAE SFKCCLLLSM IPPDIVGPKM DPEQLYEAVT ILLSLQSKNG GVTAWEPARG
QEWLELLNPT EVFADIVVEH EYNECTSSAI QALILFKQLY PNHRTEEINT SIKKAVQYIE
SIQMLDGSWY GSWGVCFTYS TWFGLGGLAA AGKTYNNCLA MRKGVHFLLT TQKDNGGWGE
SYLSCPKKRY IPSEGERSNL VQTSWAMMGL LHAGQAERDP SPLHRAAKLL INSQLENGDF
PQQEITGAFM KNCLLHYAAY RNIFPVWALA EYRRRVPLPY EKPSTERRS
