LUP5_ARATH
ID LUP5_ARATH Reviewed; 763 AA.
AC Q9FZI2;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Lupeol synthase 5;
DE Short=AtLUP5;
DE EC=5.4.99.-;
GN Name=LUP5; OrderedLocusNames=At1g66960; ORFNames=F1O19.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Benveniste P., Nave P., Schaller H.;
RT "Triterpene synthases from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=11247608; DOI=10.1023/a:1006476123930;
RA Husselstein-Muller T., Schaller H., Benveniste P.;
RT "Molecular cloning and expression in yeast of 2,3-oxidosqualene-
RT triterpenoid cyclases from Arabidopsis thaliana.";
RL Plant Mol. Biol. 45:75-92(2001).
RN [6]
RP FUNCTION.
RX DOI=10.1351/pac200375020369;
RA Ebizuka Y., Katsube Y., Tsutsumi T., Kushiro T., Shibuya M.;
RT "Functional genomics approach to the study of triterpene biosynthesis.";
RL Pure Appl. Chem. 75:369-374(2003).
CC -!- FUNCTION: Multifunctional enzyme that converts oxidosqualene to
CC tirucalla-7,21-diene-3beta-ol and two other triterpene monoalcohols.
CC {ECO:0000269|Ref.6}.
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; AF489920; AAN77001.1; -; mRNA.
DR EMBL; AC007152; AAF98208.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34578.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60852.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60854.1; -; Genomic_DNA.
DR EMBL; AY062741; AAL32819.1; -; mRNA.
DR EMBL; BT000138; AAN15457.1; -; mRNA.
DR PIR; E96693; E96693.
DR RefSeq; NP_001323105.1; NM_001334260.1.
DR RefSeq; NP_001323107.1; NM_001334261.1.
DR RefSeq; NP_176868.1; NM_105367.3.
DR AlphaFoldDB; Q9FZI2; -.
DR SMR; Q9FZI2; -.
DR STRING; 3702.AT1G66960.1; -.
DR PaxDb; Q9FZI2; -.
DR PRIDE; Q9FZI2; -.
DR ProteomicsDB; 238506; -.
DR EnsemblPlants; AT1G66960.1; AT1G66960.1; AT1G66960.
DR EnsemblPlants; AT1G66960.3; AT1G66960.3; AT1G66960.
DR EnsemblPlants; AT1G66960.4; AT1G66960.4; AT1G66960.
DR GeneID; 843014; -.
DR Gramene; AT1G66960.1; AT1G66960.1; AT1G66960.
DR Gramene; AT1G66960.3; AT1G66960.3; AT1G66960.
DR Gramene; AT1G66960.4; AT1G66960.4; AT1G66960.
DR KEGG; ath:AT1G66960; -.
DR Araport; AT1G66960; -.
DR TAIR; locus:2019703; AT1G66960.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_009074_2_1_1; -.
DR InParanoid; Q9FZI2; -.
DR OMA; ALVIFKQ; -.
DR PhylomeDB; Q9FZI2; -.
DR PRO; PR:Q9FZI2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FZI2; baseline and differential.
DR Genevisible; Q9FZI2; AT.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0042300; F:beta-amyrin synthase activity; IBA:GO_Central.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IBA:GO_Central.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 2: Evidence at transcript level;
KW Isomerase; Reference proteome; Repeat.
FT CHAIN 1..763
FT /note="Lupeol synthase 5"
FT /id="PRO_0000366135"
FT REPEAT 149..190
FT /note="PFTB 1"
FT REPEAT 515..556
FT /note="PFTB 2"
FT REPEAT 592..632
FT /note="PFTB 3"
FT REPEAT 641..682
FT /note="PFTB 4"
FT REPEAT 703..744
FT /note="PFTB 5"
FT ACT_SITE 486
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 763 AA; 87623 MW; 4095EB93A64E6755 CRC64;
MWRLKVGEGK GKDPYLFSSN NFVGRQTWEF DPKAGTREER TAVEEARRSF FDNRSRVKPS
SDLLWKMQFL KEAKFEQVIP PVKIDGGEAI TYEKATNALR RGVAFLSALQ ASDGHWPGEF
TGPLCMLPPL VFCLYITGHL EEVFDAEHRK EMLRYIYCHQ NEDGGWGFHI ESKSIMFTTT
LNYICLRILG VGPDGGLENA CKRARQWILS HGGVIYIPCW GKVWLSVLGI YDWSGVNPMP
PEIWLLPYFL PIHLGKAFSY TRITYMPISY LYGKKFVGQI TPLIMQLREE LHLQPYEEIN
WNKARHLCAK EDKYYPHPLV QDLIWDALHT FVEPLLASWP INKLVRKKAL QVAMKHIHYE
DENSHYITIG CIEKNLCMLA CWIDNPDGNH FKKHLSRIPD MMWVAEDGMK MQCFGSQLWM
TGFAVQALLA SDPRDETYDV LRRAHDYIKK SQVRDNPSGD FKSMYRHISK GGWTLSDRDH
GWQVSDCTAE AAKCCMLLST MPTDITGEKI NLEQLYDSVN LMLSLQSENG GFTAWEPVRA
YKWMELMNPT DLFANAMTER EYTECTSAVL QALVIFNQLY PDHRTKEITK SIEKAVQFIE
SKQLRDGSWY GSWGICFTYG TWFALCGLAA IGKTYNNCLS MRDGVHFLLN IQNEDGGWGE
SYMSCPEQRY IPLEGNRSNV VQTAWAMMAL IHAGQAKRDL IPLHSAAKFI ITSQLENGDF
PQQELLGASM STCMLHYSTY KDIFPPWALA EYRKAAFIHH ADL
