ID   LUPS_BETPL              Reviewed;         755 AA.
AC   Q8W3Z2;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Lupeol synthase;
DE            EC=5.4.99.41;
GN   Name=OSCBPW;
OS   Betula platyphylla (Asian white birch).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fagales; Betulaceae; Betula.
OX   NCBI_TaxID=78630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=12736505; DOI=10.1248/bpb.26.642;
RA   Zhang H., Shibuya M., Yokota S., Ebizuka Y.;
RT   "Oxidosqualene cyclases from cell suspension cultures of Betula platyphylla
RT   var. japonica: molecular evolution of oxidosqualene cyclases in higher
RT   plants.";
RL   Biol. Pharm. Bull. 26:642-650(2003).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of lupeol.
CC       {ECO:0000269|PubMed:12736505}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = lupeol; Xref=Rhea:RHEA:31383,
CC         ChEBI:CHEBI:6570, ChEBI:CHEBI:15441; EC=5.4.99.41;
CC         Evidence={ECO:0000269|PubMed:12736505};
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB055511; BAB83087.1; -; mRNA.
DR   AlphaFoldDB; Q8W3Z2; -.
DR   SMR; Q8W3Z2; -.
DR   KEGG; ag:BAB83087; -.
DR   BRENDA; 5.4.99.41; 9789.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0042299; F:lupeol synthase activity; IDA:UniProtKB.
DR   GO; GO:0019745; P:pentacyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..755
FT                   /note="Lupeol synthase"
FT                   /id="PRO_0000413994"
FT   REPEAT          146..187
FT                   /note="PFTB 1"
FT   REPEAT          511..552
FT                   /note="PFTB 2"
FT   REPEAT          588..628
FT                   /note="PFTB 3"
FT   REPEAT          637..678
FT                   /note="PFTB 4"
FT   ACT_SITE        482
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   755 AA;  86473 MW;  6AA702214DECBDCA CRC64;
     MWKLKIAEGG PGLVSGNDFI GRQHWEFDPD AGTPQERAEV EKVREEFTKN RFQMKQSADL
     LMRMQLRKEN PCQPIPPPVK VKETEVITEE AVITTLRRSL SFYSSIQAHD GHWPGESAGP
     LFFLQPFVMA LYITGDLNTI FSPAHQKEII RYLYNHQNED GGWGFHIEGH STMFGSALSY
     IALRILGEGL EDGEDGAMAK SRKWILDHGG LVAIPSWGKF WVTVLGLYEW SGCNPLPPEF
     WFLPDIFPIH PGKMLCYCRL VYMPMSYLYG KRFVGPITGL IQSLRQELYN EPYHQINWNK
     ARSTVAKEDL YYPHPLIQDL LWGFLHHVAE PVLTRWPFSM LREKALKAAI GHVHYEDENS
     KYLCIGSVEK VLCLIACWAE DPNGEAYKLH LGRIPDNYWV AEDGLKIQSF GCQMWDAGFA
     IQAILSCNLN EEYWPTLRKA HEFVKASQVP ENPSGDFKAM YRHINKGAWT FSMQDHGWQV
     SDCTAEGLKV AILFSQMPPD LVGEKIEKER LYDAVNVILS LQSSNGGFPA WEPQRAYGWL
     EKFNPTEFFE DTLIEREYVE CTSPAVHGLA LFRKFYPRHR GTEIDSSIYR GIQYIEDVQE
     PDGSWYGHWG ICYTYGTWFA VGALAACGRN YKNCPALRKS CEFLLSKQLP NGGWGESYLS
     SQNKVWTNIE GNRANLVQTA WALLSLIDAR QAEIDPTPIH RGVRVLINSQ MEDGDFPQQE
     ITGVFMRNCT LNYSSYRNIF PIWALGEYRR RVLFA