ID   LUPS_GLYGL              Reviewed;         758 AA.
AC   Q764T8;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Lupeol synthase;
DE            Short=GgLUS1;
DE            EC=5.4.99.41;
GN   Name=LUS1;
OS   Glycyrrhiza glabra (Licorice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Galegeae; Glycyrrhiza.
OX   NCBI_TaxID=49827;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL
RP   STAGE, INDUCTION, AND TISSUE SPECIFICITY.
RX   PubMed=15256745; DOI=10.1248/bpb.27.1086;
RA   Hayashi H., Huang P., Takada S., Obinata M., Inoue K., Shibuya M.,
RA   Ebizuka Y.;
RT   "Differential expression of three oxidosqualene cyclase mRNAs in
RT   Glycyrrhiza glabra.";
RL   Biol. Pharm. Bull. 27:1086-1092(2004).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of lupeol.
CC       Required for the production of betulinic acid.
CC       {ECO:0000269|PubMed:15256745}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = lupeol; Xref=Rhea:RHEA:31383,
CC         ChEBI:CHEBI:6570, ChEBI:CHEBI:15441; EC=5.4.99.41;
CC         Evidence={ECO:0000269|PubMed:15256745};
CC   -!- TISSUE SPECIFICITY: Expressed in root nodules.
CC       {ECO:0000269|PubMed:15256745}.
CC   -!- DEVELOPMENTAL STAGE: Low expression in developing seedlings.
CC       {ECO:0000269|PubMed:15256745}.
CC   -!- INDUCTION: Down-regulated by methyl jasmonate (MeJA). No effect of
CC       gibberellin A3 (GA3). {ECO:0000269|PubMed:15256745}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB116228; BAD08587.1; -; mRNA.
DR   AlphaFoldDB; Q764T8; -.
DR   SMR; Q764T8; -.
DR   KEGG; ag:BAD08587; -.
DR   BRENDA; 5.4.99.41; 2487.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0042299; F:lupeol synthase activity; IDA:UniProtKB.
DR   GO; GO:0019745; P:pentacyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..758
FT                   /note="Lupeol synthase"
FT                   /id="PRO_0000413995"
FT   REPEAT          149..190
FT                   /note="PFTB 1"
FT   REPEAT          511..552
FT                   /note="PFTB 2"
FT   REPEAT          588..628
FT                   /note="PFTB 3"
FT   REPEAT          637..678
FT                   /note="PFTB 4"
FT   ACT_SITE        482
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   758 AA;  86688 MW;  112C6627EA625DD1 CRC64;
     MWKLKIGEGG AGLISVNNFI GRQHWEFDPN AGTPQEHAEI ERLRREFTKN RFSIKQSADL
     LMRMQLRKEN HYGTNNNIPA AVKLSDAENI TVEALVTTIT RAISFYSSIQ AHDGHWPAES
     AGPLFFLQPL VMALYITGSL DDVLGPEHKK EIVRYLYNHQ NEDGGWGFHI EGHSTMFGSA
     LSYVALRILG EGPQDKAMAK GRKWILDHGG LVAIPSWGKF WVTVLGAYEW SGCNPLPPEL
     WLLPKFAPFH PGKMLCYCRL VYMPMSYLYG KKFVGPITAL IRSLREELYN EPYNQINWNT
     ARNTVAKEDL YYPHPLIQDM LWGFLYHVGE RFLNCWPFSM LRRKALEIAI NHVHYEDENS
     RYLCIGSVEK VLCLIARWVE DPNSEAYKLH LARIPDYFWL AEDGLKIQSF GCQMWDAAFA
     IQAILACNVS EEYGPTLRKA HHFVKASQVR ENPSGDFNAM YRHISKGAWT FSMHDHGWQV
     SDCTAEGLKA ALLLSEMPSE LVGGKMETER FYDAVNVILS LQSSNGGFPA WEPQKAYRWL
     EKFNPTEFFE DTMIEREYVE CTGSAMQGLA LFRKQFPQHR SKEIDRCIAK AIRYIENMQN
     PDGSWYGCWG ICYTYGTWFA VEGLTACGKN CHNSLSLRKA CQFLLSKQLP NAGWGESYLS
     SQNKVYTNLE GNRANLVQSS WALLSLTHAG QAEIDPTPIH RGMKLLINSQ MEDGDFPQQE
     ITGVFMRNCT LNYSSYRNIF PIWAMGEYRR QVLCAHSY