ID   LUPS_RICCO              Reviewed;         769 AA.
AC   Q2XPU7;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Lupeol synthase;
DE            EC=5.4.99.41;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC   Ricinus.
OX   NCBI_TaxID=3988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL
RP   STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=16445885; DOI=10.1016/j.abb.2005.12.013;
RA   Guhling O., Hobl B., Yeats T., Jetter R.;
RT   "Cloning and characterization of a lupeol synthase involved in the
RT   synthesis of epicuticular wax crystals on stem and hypocotyl surfaces of
RT   Ricinus communis.";
RL   Arch. Biochem. Biophys. 448:60-72(2006).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of the
CC       cuticular lupeol of glaucous individuals, but not of the glossy
CC       phenotype. {ECO:0000269|PubMed:16445885}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = lupeol; Xref=Rhea:RHEA:31383,
CC         ChEBI:CHEBI:6570, ChEBI:CHEBI:15441; EC=5.4.99.41;
CC         Evidence={ECO:0000269|PubMed:16445885};
CC   -!- TISSUE SPECIFICITY: Expressed in stems and laves of glaucous
CC       individuals. Not expressed neither in stems or leaves of the glossy
CC       phenotype, nor in roots of both phenotypes.
CC       {ECO:0000269|PubMed:16445885}.
CC   -!- DEVELOPMENTAL STAGE: Expressed between 6 and 50 days after emergence,
CC       with a peak at day 12. {ECO:0000269|PubMed:16445885}.
CC   -!- MISCELLANEOUS: Lupeol forms probably the thread-shaped epicuticular wax
CC       crystals that hamper insect adhesion and can prevent herbivores from
CC       climbing vertical plant structures, a phenomenon known as the 'greasy
CC       pole syndrome'. {ECO:0000305|PubMed:16445885}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Get a grip - Issue 135 of
CC       January 2012;
CC       URL="https://web.expasy.org/spotlight/back_issues/135";
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DR   EMBL; DQ268869; ABB76766.1; -; mRNA.
DR   RefSeq; NP_001310684.1; NM_001323755.1.
DR   AlphaFoldDB; Q2XPU7; -.
DR   SMR; Q2XPU7; -.
DR   STRING; 3988.XP_002527337.1; -.
DR   GeneID; 8280320; -.
DR   KEGG; ag:ABB76766; -.
DR   KEGG; rcu:8280320; -.
DR   eggNOG; KOG0497; Eukaryota.
DR   OrthoDB; 365003at2759; -.
DR   BRENDA; 5.4.99.41; 1204.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0042299; F:lupeol synthase activity; IDA:UniProtKB.
DR   GO; GO:0042335; P:cuticle development; IDA:UniProtKB.
DR   GO; GO:0019745; P:pentacyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..769
FT                   /note="Lupeol synthase"
FT                   /id="PRO_0000413996"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          513..558
FT                   /note="PFTB 2"
FT   REPEAT          590..630
FT                   /note="PFTB 3"
FT   REPEAT          639..680
FT                   /note="PFTB 4"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   769 AA;  88321 MW;  02BC53319F087E92 CRC64;
     MWRIKIAEGG NNPYIYSTNN FQGRQIWVFD PNAGTPEEQA EVEEARQNFW KNRFQVKPNS
     DLLWQLQFLR EKNFKQKIPK VKVEDGEEIT SEIAAAALRR SVHLFSALQA SDGHWCAENG
     GLLFFLPPLV FAVYITGHLN TVFSPEHRKE ILRYIYCHQN EDGGWGIHIE GHSTMFCTVL
     NYICMRILGE ARDGGIENAC ERGRKWILDH GGATGISSWG KTWLSILGVY EWDGTNPMPP
     EFWAFPSSFP LHPAKMFCYC RITYMPMSYL YGKRFVGPIT PLILQIREEI YNEPYNKIKW
     NSVRHLCAKE DNYFPHPTIQ KLLWDALYTF SEPLFSRWPF NKLREKALKI TMDHIHYEDH
     NSRYITIGCV EKPLCMLACW IEDPHGEAFK KHLARIADYI WVGEDGIKMQ SFGSQTWDTS
     LALQALIASD LSHEIGPTLK QGHVFTKNSQ ATENPSGDFR KMFRHISKGA WTFSDKDQGW
     QVSDCTAESL KCCLLFSMMP PEIVGEKMEP EKVYDSVNVI LSLQSQNGGF TAWEPARAGS
     WMEWLNPVEF MEDLVVEHEY VECTSSAIQA LVLFKKLYPR HRNKEIENCI INAAQFIENI
     QEPDGSWYGN WGICFSYGTW FALKGLAAAG RTYENCSAIR KGVDFLLKSQ RDDGGWAESY
     LSCPKKVYVP FEGNRSNLVQ TAWAMMGLIY GGQAKRDPMP LHRAAKLLIN SQTDLGDFPQ
     QELTGAFMRN CMLHYALFRN TFPIWALAEY RRHVLFPSAG FGFGFTNNL