LURA1_HUMAN
ID LURA1_HUMAN Reviewed; 239 AA.
AC Q96LR2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Leucine rich adaptor protein 1;
DE AltName: Full=Leucine repeat adapter protein 35A;
GN Name=LURAP1; Synonyms=C1orf190, LRAP35A, LRP35A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19369195; DOI=10.1074/mcp.m800588-mcp200;
RA Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,
RA Mann M., Daub H.;
RT "Large-scale proteomics analysis of the human kinome.";
RL Mol. Cell. Proteomics 8:1751-1764(2009).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21048106; DOI=10.4049/jimmunol.0903869;
RA Jing Z., Yuan X., Zhang J., Huang X., Zhang Z., Liu J., Zhang M., Oyang J.,
RA Zhang Y., Zhang Z., Yang R.;
RT "Chromosome 1 open reading frame 190 promotes activation of NF-kappaB
RT canonical pathway and resistance of dendritic cells to tumor-associated
RT inhibition in vitro.";
RL J. Immunol. 185:6719-6727(2010).
CC -!- FUNCTION: Acts as an activator of the canonical NF-kappa-B pathway and
CC drive the production of pro-inflammatory cytokines. Promotes the
CC antigen (Ag)-presenting and priming function of dendritic cells via the
CC canonical NF-kappa-B pathway (PubMed:21048106). In concert with MYO18A
CC and CDC42BPA/CDC42BPB, is involved in modulating lamellar actomyosin
CC retrograde flow that is crucial to cell protrusion and migration.
CC Activates CDC42BPA/CDC42BPB and targets it to actomyosin through its
CC interaction with MYO18A, leading to MYL9/MLC2 phosphorylation and
CC MYH9/MYH10-dependent actomyosin assembly in the lamella (By
CC similarity). {ECO:0000250|UniProtKB:D4A8G3,
CC ECO:0000269|PubMed:21048106}.
CC -!- SUBUNIT: Forms a tripartite complex with CDC42BPA/CDC42BPB and MYO18A
CC acting as an adapter connecting both. Its binding to CDC42BPA/CDC42BPB
CC results in their activation by abolition of their negative
CC autoregulation. Interacts with CDC42BPA and CDC42BPB.
CC {ECO:0000250|UniProtKB:D4A8G3}.
CC -!- INTERACTION:
CC Q96LR2; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-741355, EBI-11096309;
CC Q96LR2; A2BDD9: AMOT; NbExp=5; IntAct=EBI-741355, EBI-17286414;
CC Q96LR2; Q4VCS5-2: AMOT; NbExp=3; IntAct=EBI-741355, EBI-3891843;
CC Q96LR2; P48730: CSNK1D; NbExp=4; IntAct=EBI-741355, EBI-751621;
CC Q96LR2; O75190-2: DNAJB6; NbExp=3; IntAct=EBI-741355, EBI-12593112;
CC Q96LR2; O60941-5: DTNB; NbExp=5; IntAct=EBI-741355, EBI-11984733;
CC Q96LR2; Q9H0I2: ENKD1; NbExp=3; IntAct=EBI-741355, EBI-744099;
CC Q96LR2; P22607: FGFR3; NbExp=3; IntAct=EBI-741355, EBI-348399;
CC Q96LR2; Q14957: GRIN2C; NbExp=3; IntAct=EBI-741355, EBI-8285963;
CC Q96LR2; P06396: GSN; NbExp=3; IntAct=EBI-741355, EBI-351506;
CC Q96LR2; Q96CS2: HAUS1; NbExp=4; IntAct=EBI-741355, EBI-2514791;
CC Q96LR2; O14964: HGS; NbExp=4; IntAct=EBI-741355, EBI-740220;
CC Q96LR2; Q9NP66: HMG20A; NbExp=5; IntAct=EBI-741355, EBI-740641;
CC Q96LR2; P01112: HRAS; NbExp=3; IntAct=EBI-741355, EBI-350145;
CC Q96LR2; O14901: KLF11; NbExp=3; IntAct=EBI-741355, EBI-948266;
CC Q96LR2; P12035: KRT3; NbExp=3; IntAct=EBI-741355, EBI-2430095;
CC Q96LR2; Q8WWY3: PRPF31; NbExp=6; IntAct=EBI-741355, EBI-1567797;
CC Q96LR2; Q9NUL5-4: SHFL; NbExp=3; IntAct=EBI-741355, EBI-11955083;
CC Q96LR2; P06753: TPM3; NbExp=4; IntAct=EBI-741355, EBI-355607;
CC Q96LR2; Q8N3L3: TXLNB; NbExp=7; IntAct=EBI-741355, EBI-6116822;
CC Q96LR2; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-741355, EBI-740727;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21048106}.
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DR EMBL; AK057892; BAB71608.1; -; mRNA.
DR EMBL; AL672043; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC034422; AAH34422.1; -; mRNA.
DR CCDS; CCDS30703.1; -.
DR RefSeq; NP_001013633.1; NM_001013615.2.
DR AlphaFoldDB; Q96LR2; -.
DR SMR; Q96LR2; -.
DR BioGRID; 139071; 240.
DR IntAct; Q96LR2; 32.
DR STRING; 9606.ENSP00000361048; -.
DR iPTMnet; Q96LR2; -.
DR PhosphoSitePlus; Q96LR2; -.
DR BioMuta; LURAP1; -.
DR DMDM; 74752014; -.
DR EPD; Q96LR2; -.
DR MassIVE; Q96LR2; -.
DR PaxDb; Q96LR2; -.
DR PeptideAtlas; Q96LR2; -.
DR PRIDE; Q96LR2; -.
DR ProteomicsDB; 77237; -.
DR Antibodypedia; 32773; 65 antibodies from 14 providers.
DR DNASU; 541468; -.
DR Ensembl; ENST00000371980.4; ENSP00000361048.3; ENSG00000171357.6.
DR GeneID; 541468; -.
DR KEGG; hsa:541468; -.
DR MANE-Select; ENST00000371980.4; ENSP00000361048.3; NM_001013615.3; NP_001013633.1.
DR UCSC; uc010oma.3; human.
DR CTD; 541468; -.
DR DisGeNET; 541468; -.
DR GeneCards; LURAP1; -.
DR HGNC; HGNC:32327; LURAP1.
DR HPA; ENSG00000171357; Low tissue specificity.
DR MIM; 616129; gene.
DR neXtProt; NX_Q96LR2; -.
DR OpenTargets; ENSG00000171357; -.
DR PharmGKB; PA142672401; -.
DR VEuPathDB; HostDB:ENSG00000171357; -.
DR eggNOG; ENOG502QQFH; Eukaryota.
DR GeneTree; ENSGT00530000063790; -.
DR HOGENOM; CLU_066656_1_0_1; -.
DR InParanoid; Q96LR2; -.
DR OMA; PCPEMDW; -.
DR OrthoDB; 1385244at2759; -.
DR PhylomeDB; Q96LR2; -.
DR TreeFam; TF332089; -.
DR PathwayCommons; Q96LR2; -.
DR SignaLink; Q96LR2; -.
DR BioGRID-ORCS; 541468; 31 hits in 1061 CRISPR screens.
DR GenomeRNAi; 541468; -.
DR Pharos; Q96LR2; Tdark.
DR PRO; PR:Q96LR2; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q96LR2; protein.
DR Bgee; ENSG00000171357; Expressed in C1 segment of cervical spinal cord and 107 other tissues.
DR Genevisible; Q96LR2; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0001819; P:positive regulation of cytokine production; IDA:UniProtKB.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IDA:UniProtKB.
DR GO; GO:0009966; P:regulation of signal transduction; IBA:GO_Central.
DR InterPro; IPR039499; LURA1/LRA25.
DR InterPro; IPR037443; LURAP1.
DR PANTHER; PTHR33767; PTHR33767; 1.
DR Pfam; PF14854; LURAP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Leucine-rich repeat; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..239
FT /note="Leucine rich adaptor protein 1"
FT /id="PRO_0000271078"
FT REPEAT 55..83
FT /note="LRR 1"
FT REPEAT 93..114
FT /note="LRR 2"
FT REGION 107..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 118
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9D6I9"
FT MOD_RES 126
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9D6I9"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:D4A8G3"
FT MOD_RES 213
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19369195"
SQ SEQUENCE 239 AA; 25806 MW; E6D06F384B5448C1 CRC64;
MEGTVESQTP DLRDVEGKVG RKTPEGLLRG LRGECELGTS GALLLPGASS TGHDLGDKIM
ALKMELAYLR AIDVKILQQL VTLNEGIEAV RWLLEERGTL TSHCSSLTSS QYSLTGGSPG
RSRRGSWDSL PDTSTTDRLD SVSIGSFLDT VAPSELDEQG PPGAPRSEMD WAKVIAGGER
ARTEVDVAAT RLGSLRAVWK PPGERLQGGP PESPEDESAK LGFEAHWFWE QCQDDVTFL
