LUTA_BACSU
ID LUTA_BACSU Reviewed; 238 AA.
AC O07020; Q795K4;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Lactate utilization protein A;
GN Name=lutA; Synonyms=yvfV; OrderedLocusNames=BSU34050;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Denizot F.;
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION IN LACTATE UTILIZATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=3610;
RX PubMed=19201793; DOI=10.1128/jb.01464-08;
RA Chai Y., Kolter R., Losick R.;
RT "A widely conserved gene cluster required for lactate utilization in
RT Bacillus subtilis and its involvement in biofilm formation.";
RL J. Bacteriol. 191:2423-2430(2009).
CC -!- FUNCTION: Is essential for L-lactate degradation and allows cells to
CC grow with lactate as the sole carbon source. May also allow cells to
CC utilize an alternative carbon source during biofilm formation, since it
CC contributes to the formation of architecturally complex communities
CC when lactate is present. {ECO:0000269|PubMed:19201793}.
CC -!- INDUCTION: Is under the dual control of the transcriptional repressors
CC LutR and SinR, which allows the lutABC operon to be induced during both
CC growth in liquid culture and biofilm formation. Is induced by L-
CC lactate, which relieves LutR repression. {ECO:0000269|PubMed:19201793}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are unable to grow on
CC minimal medium with L-lactate as the sole carbon source. Cells lacking
CC the lutABC operon exhibit little or no defect in biofilm formation on
CC MSgg medium, but form small colonies that almost completely lacked
CC surface architecture when glycerol is replaced with L-lactate in the
CC MSgg medium. {ECO:0000269|PubMed:19201793}.
CC -!- SIMILARITY: Belongs to the LutA/YkgE family. {ECO:0000305}.
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DR EMBL; Z94043; CAB07992.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15410.1; -; Genomic_DNA.
DR PIR; E70039; E70039.
DR RefSeq; NP_391285.1; NC_000964.3.
DR RefSeq; WP_003244353.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O07020; -.
DR SMR; O07020; -.
DR STRING; 224308.BSU34050; -.
DR jPOST; O07020; -.
DR PaxDb; O07020; -.
DR PRIDE; O07020; -.
DR EnsemblBacteria; CAB15410; CAB15410; BSU_34050.
DR GeneID; 936297; -.
DR KEGG; bsu:BSU34050; -.
DR PATRIC; fig|224308.179.peg.3691; -.
DR eggNOG; COG0247; Bacteria.
DR InParanoid; O07020; -.
DR OMA; NSCLMHI; -.
DR PhylomeDB; O07020; -.
DR BioCyc; BSUB:BSU34050-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004459; F:L-lactate dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0006089; P:lactate metabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02105; LutA; 1.
DR InterPro; IPR004017; Cys_rich_dom.
DR InterPro; IPR022822; LutA.
DR Pfam; PF02754; CCG; 2.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..238
FT /note="Lactate utilization protein A"
FT /id="PRO_0000384045"
SQ SEQUENCE 238 AA; 26393 MW; 2FCB82A5545C6169 CRC64;
MKVSLFVTCL VDMFQTNVGK ATVELLERLG CEVDFPEGQI CCGQPAYNSG YVHDAKKAMK
RMIETFQDSE YVVSPSGSCT TMFREYPHLF QDDPKWADKA KKLADKTYEL TDFIVNVLGV
EDVGATLHTK ATLHTSCHMT RLLGVRKEPM KLLSHVKGLQ FTELPGKHNC CGFGGTFSVK
MAQISEQMVD EKVECVEETG AEVLIGADCG CLMNIGGRLG RKDKNVKVMH IAEVLNSR
