ID   LUTC_BACSU              Reviewed;         240 AA.
AC   O32259;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Lactate utilization protein C;
GN   Name=lutC; Synonyms=yvbY; OrderedLocusNames=BSU34030;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION IN LACTATE UTILIZATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=3610;
RX   PubMed=19201793; DOI=10.1128/jb.01464-08;
RA   Chai Y., Kolter R., Losick R.;
RT   "A widely conserved gene cluster required for lactate utilization in
RT   Bacillus subtilis and its involvement in biofilm formation.";
RL   J. Bacteriol. 191:2423-2430(2009).
CC   -!- FUNCTION: Is essential for L-lactate degradation and allows cells to
CC       grow with lactate as the sole carbon source. May also allow cells to
CC       utilize an alternative carbon source during biofilm formation, since it
CC       contributes to the formation of architecturally complex communities
CC       when lactate is present. {ECO:0000269|PubMed:19201793}.
CC   -!- INDUCTION: Is under the dual control of the transcriptional repressors
CC       LutR and SinR, which allows the lutABC operon to be induced during both
CC       growth in liquid culture and biofilm formation. Is induced by L-
CC       lactate, which relieves LutR repression. {ECO:0000269|PubMed:19201793}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are unable to grow on
CC       minimal medium with L-lactate as the sole carbon source. Cells lacking
CC       the lutABC operon exhibit little or no defect in biofilm formation on
CC       MSgg medium, but form small colonies that almost completely lacked
CC       surface architecture when glycerol is replaced with L-lactate in the
CC       MSgg medium. {ECO:0000269|PubMed:19201793}.
CC   -!- SIMILARITY: Belongs to the LutC/YkgG family. {ECO:0000305}.
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DR   EMBL; AL009126; CAB15408.1; -; Genomic_DNA.
DR   PIR; A70031; A70031.
DR   RefSeq; NP_391283.1; NC_000964.3.
DR   RefSeq; WP_003228309.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O32259; -.
DR   SMR; O32259; -.
DR   IntAct; O32259; 1.
DR   MINT; O32259; -.
DR   STRING; 224308.BSU34030; -.
DR   jPOST; O32259; -.
DR   PaxDb; O32259; -.
DR   PRIDE; O32259; -.
DR   EnsemblBacteria; CAB15408; CAB15408; BSU_34030.
DR   GeneID; 937742; -.
DR   KEGG; bsu:BSU34030; -.
DR   PATRIC; fig|224308.179.peg.3689; -.
DR   eggNOG; COG1556; Bacteria.
DR   InParanoid; O32259; -.
DR   OMA; PKTYIAI; -.
DR   PhylomeDB; O32259; -.
DR   BioCyc; BSUB:BSU34030-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0006089; P:lactate metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10420; -; 1.
DR   HAMAP; MF_02104; LutC; 1.
DR   InterPro; IPR024185; FTHF_cligase-like_sf.
DR   InterPro; IPR003741; LUD_dom.
DR   InterPro; IPR022823; LutC.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   Pfam; PF02589; LUD_dom; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..240
FT                   /note="Lactate utilization protein C"
FT                   /id="PRO_0000360219"
SQ   SEQUENCE   240 AA;  26277 MW;  DA528E28BF8A3F18 CRC64;
     MTKGTIQNQE SFLNRIASSL GRERRTGGVA VPEWAHQPQY KTLEGYSADD LVTVLKNHCV
     KIHTELIETD STGLYDALRE QVSRFSGGPV IIPKDPRFEE YGLKSLLTKD WPSEGTPVWE
     WDADKGEENI KKAEQANVGI TFSEITLAES GTVVLFSSKD IGRSVSLLPT TYIAIVPKSS
     IVPRMTQASD IIRQNIANGV TVPSCINYIT GPSNSADIEM DLVVGVHGPV KAAYILVSDR