LUTP_BACSU
ID LUTP_BACSU Reviewed; 563 AA.
AC P71067;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=L-lactate permease;
GN Name=lutP; Synonyms=yvfH; OrderedLocusNames=BSU34190;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Denizot F.;
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 51-563.
RC STRAIN=168;
RX PubMed=8969506; DOI=10.1099/13500872-142-11-3089;
RA Fabret C., Quentin Y., Chapal N., Guiseppi A., Haiech J., Denizot F.;
RT "Integrated mapping and sequencing of a 115 kb DNA fragment from Bacillus
RT subtilis: sequence analysis of a 21 kb segment containing the sigL locus.";
RL Microbiology 142:3089-3096(1996).
RN [4]
RP FUNCTION AS A LACTATE PERMEASE, AND DISRUPTION PHENOTYPE.
RC STRAIN=3610;
RX PubMed=19201793; DOI=10.1128/jb.01464-08;
RA Chai Y., Kolter R., Losick R.;
RT "A widely conserved gene cluster required for lactate utilization in
RT Bacillus subtilis and its involvement in biofilm formation.";
RL J. Bacteriol. 191:2423-2430(2009).
CC -!- FUNCTION: Is the principal permease for the uptake of L-lactate in
CC B.subtilis. {ECO:0000269|PubMed:19201793}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are markedly impaired
CC (but not totally blocked) in growth on L-lactate minimal medium but not
CC impaired in growth on glucose minimal medium.
CC {ECO:0000269|PubMed:19201793}.
CC -!- SIMILARITY: Belongs to the lactate permease family. {ECO:0000305}.
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DR EMBL; Z94043; CAB08002.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15424.1; -; Genomic_DNA.
DR EMBL; Z71928; CAA96486.1; -; Genomic_DNA.
DR PIR; A70038; A70038.
DR RefSeq; NP_391299.1; NC_000964.3.
DR RefSeq; WP_003228275.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; P71067; -.
DR STRING; 224308.BSU34190; -.
DR TCDB; 2.A.14.1.3; the lactate permease (lctp) family.
DR PaxDb; P71067; -.
DR PRIDE; P71067; -.
DR EnsemblBacteria; CAB15424; CAB15424; BSU_34190.
DR GeneID; 936322; -.
DR KEGG; bsu:BSU34190; -.
DR PATRIC; fig|224308.179.peg.3706; -.
DR eggNOG; COG1620; Bacteria.
DR InParanoid; P71067; -.
DR OMA; LFVYKMP; -.
DR PhylomeDB; P71067; -.
DR BioCyc; BSUB:BSU34190-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015129; F:lactate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015295; F:solute:proton symporter activity; IBA:GO_Central.
DR InterPro; IPR003804; Lactate_perm.
DR PANTHER; PTHR30003; PTHR30003; 1.
DR Pfam; PF02652; Lactate_perm; 1.
DR TIGRFAMs; TIGR00795; lctP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..563
FT /note="L-lactate permease"
FT /id="PRO_0000210381"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 542..562
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 563 AA; 59762 MW; E3B0983059B19B08 CRC64;
MQWTQAYTPI GGNLLLSALA ALVPIIFFFW ALAIKRMKGY TAGLATLGIA LIIAVLVYRM
PAEKALMSAT QGAVYGLLPI GWIIVTSVFL YKITVKTGQF DIIRSSVLSI TDDRRLQALL
IAFSFGAFLE GAAGFGAPVA ISAALLVGLG FNPLYAAGIC LIANTAPVAF GAIGIPITAV
EGPTGIPAME ISQMVGRQLP FLSVFIPLYL IIIMSGFRKA LEIWPAILVS GVSFAVVQYL
SSNFLGPELP DVLSALVSMA ALAVFLKWWK PKTTFRFAGE QESAASIETA RTNPAAPAYR
GGQIFKAWSP FLLLTAMISV WGIPSVKSAL TGHYEGSAVF LKWLNAVGEK LTFSPGVPFL
NNQIVNADGT PIEAVYKLEV LGSAGTAILI AAVLSKFITA ISWKDWGTVF KETVQELKLP
ILTIASVVGF AYVTNSSGMS TTLGMTLALT GSMFTFFSPV LGWLGVFITG SDTSANLLFG
NLQKVTALSV GMDPVLSVAA NSSGGVTGKM ISPQSIAVAC AAVGLAGKES DLFRFTIKHS
LFLLLLVCII TFLQHHVFSW MIP
