LUXA1_PHOLE
ID LUXA1_PHOLE Reviewed; 354 AA.
AC P09140;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Alkanal monooxygenase alpha chain;
DE EC=1.14.14.3 {ECO:0000250|UniProtKB:P24113};
DE AltName: Full=Bacterial luciferase alpha chain;
GN Name=luxA;
OS Photobacterium leiognathi.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=553611;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=554;
RX PubMed=3186447; DOI=10.1093/nar/16.20.9855;
RA Illiarinov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P.,
RA Gitelson J.I.;
RT "Nucleotide sequence of part of Photobacterium leiognathi lux region.";
RL Nucleic Acids Res. 16:9855-9855(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=554;
RX PubMed=3382442;
RA Illarionov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P.,
RA Gitelson J.I.;
RT "Nucleotide sequence of genes for alpha- and beta-subunits of luciferase
RT from Photobacterium leiognathi.";
RL Bioorg. Khim. 14:412-415(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=554;
RX PubMed=2311938; DOI=10.1016/0378-1119(90)90117-a;
RA Illarionov B.A., Blinov V.M., Donchenko A.P., Protopopova M.V.,
RA Karginov V.A., Mertvetsov N.P., Gitelson J.I.;
RT "Isolation of bioluminescent functions from Photobacterium leiognathi:
RT analysis of luxA, luxB, luxG and neighboring genes.";
RL Gene 86:89-94(1990).
CC -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC {ECO:0000250|UniProtKB:P24113}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC Evidence={ECO:0000250|UniProtKB:P24113};
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC {ECO:0000250|UniProtKB:P07740}.
CC -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; X08036; CAA30831.1; -; Genomic_DNA.
DR PIR; JQ0413; JQ0413.
DR RefSeq; WP_060988028.1; NZ_LNRC01000002.1.
DR AlphaFoldDB; P09140; -.
DR SMR; P09140; -.
DR STRING; 553611.GCA_001557755_01581; -.
DR GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR CDD; cd01096; Alkanal_monooxygenase; 1.
DR Gene3D; 3.20.20.30; -; 1.
DR InterPro; IPR033924; Alkanal_monooxygenase.
DR InterPro; IPR018235; Bacterial_luciferase_CS.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR002103; Luciferase_bac/NFP.
DR Pfam; PF00296; Bac_luciferase; 1.
DR PRINTS; PR00089; LUCIFERASE.
DR SUPFAM; SSF51679; SSF51679; 1.
DR PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE 3: Inferred from homology;
KW Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW Photoprotein.
FT CHAIN 1..354
FT /note="Alkanal monooxygenase alpha chain"
FT /id="PRO_0000220164"
SQ SEQUENCE 354 AA; 40371 MW; 569F6EEB903C7B90 CRC64;
MKFGNICFSY QPPGESHKEV MDRFVRLGVA SEELNFDTFW TLEHHFTEFG LTGNLYVACA
NILGRTKKLN VGTMGIVLPT AHPARQMEDL LLLDQMSKGR FNFGVVRGLY HKDFRVFGVT
MEDSRSITED FHKMIMDGSK SGVLHTDGKN IEFPDVNVYP EAYLDKIPTC MTAESAATTT
WLAERGLPMV LSWIITTSEK KAQMELYNEI AAEHGHDIHN IDHSMTFICS VNEDPEKAES
VCRDFLSNWY ESYTNATNIF KDSNQTRGYD YHKGQWRDFV LQGHTDTRRR LDYSNNLNPV
GTPEKCIEII QRDIDATGIN NITLGFEANG SEQEIIASME RFMTQVAPYL KDPK
